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Improved Schiff staining fluid and preparation method thereof

A preparation method and dyeing solution technology, applied in the preparation of test samples, etc., can solve the problems of toxic and harmful gases affecting the environment and personnel occupational health and safety, and the preparation process is cumbersome, achieving good dyeing effect, simplifying preparation methods, and reducing risks. Effect

Inactive Publication Date: 2019-04-30
苏州华测生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of the traditional dyeing solution used for glycogen staining is cumbersome, and equivalent hydrochloric acid needs to be prepared and boiled. The preparation of equivalent hydrochloric acid has certain risks, and toxic and harmful gases will also be produced during the boiling process, which will affect the environment and occupational health and safety of personnel.

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  • Improved Schiff staining fluid and preparation method thereof
  • Improved Schiff staining fluid and preparation method thereof
  • Improved Schiff staining fluid and preparation method thereof

Examples

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Effect test

Embodiment 1

[0022] Embodiment 1 improved Schiff staining solution formula composition and preparation method

[0023] Modified Schiff staining solution formula: pararosaniline hydrochloride 1g, activated carbon 2g, commercially available concentrated hydrochloric acid (concentration 36-38%) 1ml, sodium metabisulfite 1.5g. The solvent is distilled water.

[0024] The preparation method of the modified Schiff staining solution:

[0025] Put pararosaniline hydrochloride into 200 ml of distilled water and stir to dissolve, then add hydrochloric acid and sodium metabisulfite in turn and stir for 10 minutes, and place in a dark place overnight; then add 2 g of activated carbon, stir for 2 minutes and filter to obtain a staining solution, put Store in a brown bottle at 4°C in a refrigerator.

Embodiment 2

[0026] Example 2 Comparative study on the effect of traditional Schiff staining solution and modified Schiff staining solution on glycogen staining in liver pathological sections

[0027] Research method: 40 rat liver histopathological sections were randomly divided into two groups, namely the traditional Schiff staining solution group and the modified Schiff staining solution group, 20 slices in each group, dewaxed in xylene for 15 minutes, and hydrated with gradient alcohol. Wash with deionized water for 2 minutes, oxidize with periodic acid for 10 minutes, wash with deionized water twice, each time for 2 minutes, stain with traditional Schiff staining solution and modified Schiff staining solution for 10 minutes, rinse with tap water for 10 minutes, and soak in hematoxylin for 30 seconds , washed with tap water for 5 minutes, dehydrated with gradient alcohol, transparent with xylene, and mounted. After the xylene volatilized, the slides were read under a microscope, and the...

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Abstract

The invention discloses improved Schiff staining fluid and a preparation method thereof. Basic fuchsin in traditional Schiff staining fluid was replaced by pararosaniline hydrochloride, and 20mL equivalent hydrochloric acid was replaced by 1mL hydrochloric acid. In the preparation process, preparation of equivalent hydrochloric acid and boiling are not needed, so that preparation steps of the staining fluid can be obviously simplified; by comparing liver section glycogen staining results of the modified glycogen staining fluid and conventional staining solution, it is found that the new staining fluid is better in staining effect, and the staining results of the two methods show no statistically significant difference (P>0.05); and the preparation method of the staining fluid is simple andeasy to master, and the method can be widely applied to pathological section glycogen staining, thereby effectively preventing the risk of preparing equivalent hydrochloric acid and the harm to the preparation personnel and the preparation environment due to volatilization of toxic gas caused by heating in the conventional preparation process.

Description

technical field [0001] The invention relates to a brand-new and improved Schiff staining solution composition and a preparation method, belonging to the field of new methods of pathological technology. Background technique [0002] With the development of medical experiment technology, the application of glycogen staining is more extensive, such as to prove and identify the nature of intracellular vacuoles, the diagnosis of cardiomyopathy and other cardiovascular diseases, the diagnosis and research of glycogen accumulation diseases, diabetes Diagnosis and research of certain tumors, etc. In addition to the identification of glycogen and the display of mucus, it can also observe the coloring of glomerular basement membrane, neutral mucus substance of colonic goblet cells, amoeba trophozoites and mold, which provides a great support for clinical diagnosis, classification and treatment. important basis. However, the preparation process of the traditional staining solution fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 陈勇陆国才夏玉叶宗英丁玉芳
Owner 苏州华测生物技术有限公司