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Method and device for culturing edible fungi after inoculation

A cultivation method and technology of edible fungi, which are applied in chemical instruments and methods, botany equipment and methods, cultivation, etc., can solve the problems that culture bottles cannot cooperate with operations, achieve good stirring effect, improve cultivation efficiency, and improve convenience Effect

Pending Publication Date: 2019-05-03
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a cultivation method and device after inoculation of edible fungi. Due to the inappropriate size of the stirring paddle, it cannot cooperate with the culture bottle (usually a triangle flask)

Method used

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  • Method and device for culturing edible fungi after inoculation
  • Method and device for culturing edible fungi after inoculation
  • Method and device for culturing edible fungi after inoculation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Taking the cultivation of straw mushroom as an example, the cultivation method after inoculation includes the following steps:

[0059] (1) Sterilize the inoculation hook, use the inoculation hook to inoculate the strain block of straw mushroom from the test tube into the culture bottle;

[0060] (2) Under aseptic conditions, put the stirring device into the culture bottle, and start the stirring device (using the device in Example 4), stir and crush the bacteria block in the culture bottle, stir for 2 minutes, and the speed is 200r / min , then stop stirring, take out the stirring device, and seal the bottle mouth;

[0061] (3) Place the culture bottle in a constant temperature shaker and continue to shake at a constant temperature for further cultivation. See attached for specific results Figure 13 shown.

Embodiment 2

[0067] Taking the cultivation of Pleurotus eryngii as an example, the cultivation method after inoculation comprises the following steps:

[0068] (1) Sterilize the inoculation hook, use the inoculation hook to inoculate the strain block of Pleurotus eryngii in the test tube into the culture bottle;

[0069] (2) After the strains in the culture bottle grow for 3 days, under aseptic conditions, put the stirring device into the culture bottle, and start the stirring device (using the device in Example 5) for stirring treatment, stirring for 3 minutes, and the rotation speed is 200r / min, then stop stirring, take out the stirring device, and seal the bottle;

[0070] (3) Place the culture bottle in a constant temperature shaker, and then continue the constant temperature shaking culture. See attached for specific results Figure 14 shown.

Embodiment 3

[0072] Taking the cultivation of Flammulina velutipes as an example, the cultivation method after inoculation comprises the following steps:

[0073] (1) Sterilize the inoculation hook, use the inoculation hook to inoculate the solid strain block of Flammulina velutipes into the culture bottle;

[0074] (2) After the bacteria in the culture bottle grow for 6 days, under aseptic conditions, put the stirring device into the culture bottle, start the stirring device to stir again, stir for 4 minutes, the speed is 200r / min, and then stop stirring;

[0075] (3) Take out the stirring device, and transfer the bacterial solution in the culture bottle to the next-level seed tank. See attached for specific results Figure 15 shown.

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Abstract

The invention relates to the technical field of edible fungi culture, particularly relates to a method for culturing edible fungi after inoculation and also relates to a device used in the culture method. The method comprises the following steps of: inoculating solid strain blocks to a culture bottle, stirring and crushing solid strain blocks by using a stirring device, continuing culturing, or after inoculating and growing for a period of time, stirring and crushing solid strain blocks, continuing culturing, or after the culture bottle is well cultured, stirring and crushing by adopting the stirring device before solid strain blocks are inoculated to a next-stage seed tank, and inoculating the seed tank. According to the method and device, the telescopic stirring device is adopted to achieve the purpose of stirring and crushing strain blocks or materials in the culture bottle and preventing strain blocks or materials from being infected by miscellaneous bacteria. The stirring device is convenient to take and place, the operation is convenient, and the problem in the prior art that a stirring paddle cannot be matched with a triangular bottle because the size of the stirring paddleis not proper is solved.

Description

technical field [0001] The invention relates to the technical field of edible fungi cultivation, in particular to a method for cultivating edible fungi after inoculation, and also to a device used in the above cultivation method. Background technique [0002] When the existing edible fungus strains start liquid culture, the strain block is generally cut into small pieces, inoculated into a triangular flask filled with culture medium, and then subjected to constant temperature oscillation cultivation. In this culture method, the bacterium balls formed are usually uneven in size and the number of bacterium balls is small, which is not conducive to the inoculation and cultivation of the seed tank in the next step; therefore, a suitable stirring device is required for stirring the bacterial liquid and crushing the bacterium balls. When stirring, it is necessary to extend the stirring paddle into the triangular flask. If the stirring paddle is too small, it will not be easy to mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/60A01G18/00B01F13/10B02C13/16
CPCY02W30/40
Inventor 任海霞万鲁长任鹏飞曲玲郭惠东杨鹏
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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