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Method for culturing calluses of cremastra appendiculata

A callus, culture method technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effect of solving the shortage of medicinal material resources

Active Publication Date: 2019-05-03
CHENGDU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the successful induction of callus using rhododendron orchid scape as explants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The culture method of the rhododendron orchid callus that the present embodiment provides comprises the steps:

[0017] (1) Selection and pre-cultivation of explants: select the young and tender scapes that have not bloomed on the rhododendron orchid plant, wash them with a concentration of 2% Xiaoxiling, cut off the flower buds on it, and cut off the flower buds. Scapes are used as explants, and the explants are placed on the ultra-clean workbench, sterilized with 0.1% mercury chloride for 4 minutes, rinsed with sterile water for 3 times, and then blotted dry with sterilized filter paper The water was added to the MS0 pre-medium, and pre-cultivated for 3 days at a temperature of 15°C and without light;

[0018] (2) Callus induction culture: insert the pre-cultured explants into the callus induction medium B5+KT0.1mg L -1 +NAA0.5mg·L -1 +2,4-D1.0mg·L -1 + Thiourea 50mg·L -1 + activated carbon 0.1%, the callus induction culture is carried out under the condition that...

Embodiment 2

[0022] The culture method of the rhododendron orchid callus that the present embodiment provides comprises the steps:

[0023] (1) Selection and pre-cultivation of explants: select the young and tender scapes that have not bloomed on Rhododendron orchid plants, wash them with 3.5% Xiaoxiling, cut off the flower buds on it, and cut off the flower buds that have been cut off the flower buds. Scapes are used as explants, and the explants are placed on the ultra-clean workbench, sterilized with 0.15% mercury chloride for 8 minutes, rinsed with sterile water for 5 times, and then blotted dry with sterilized filter paper After adding water into the MS0 pre-medium, pre-cultivate for 5 days at a temperature of 18°C ​​and without light;

[0024] (2) Induction culture of callus: insert the pre-cultured explants into callus induction medium B5+KT0.5 mg·L by inserting -1 +NAA1.0mg·L -1 +2,4-D1.2mg·L -1 + Thiourea 100mg·L -1 + Activated charcoal 0.3%, carry out callus induction culture...

Embodiment 3

[0028] The culture method of the rhododendron orchid callus that the present embodiment provides comprises the steps:

[0029] (1) Selection and pre-cultivation of explants: select unflowered young and tender scapes on Rhododendron orchid plants, wash them with 5% Xiaoxiling, cut off the flower buds on it, and cut off the flower buds. Scapes are used as explants, and the explants are placed on the ultra-clean workbench, sterilized with 0.2% mercury chloride for 12 minutes, rinsed with sterile water for 6 times, and then blotted dry with sterilized filter paper The water was added to the MS0 pre-medium, and pre-cultivated for 7 days at a temperature of 22°C and without light;

[0030] (2) Induction culture of callus: insert the pre-cultured explant into callus induction medium B5+KT1.0mg·L -1 +NAA1.5mg·L -1 +2,4-D1.5mg·L -1 + Thiourea 150mg·L -1 + activated charcoal 0.5%, the temperature is 28 ℃, 10 hours of light per day, and the light intensity is 2000lx to carry out call...

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Abstract

The invention discloses a method for culturing calluses of cremastra appendiculata. The method includes the following steps of explant selection, pre-culture, induction culture of the calluses, and enrichment culture of the calluses. According to the method, a cremastra appendiculata tender scape serves as an explant, a large quantity of good-quality calluses of cremastra appendiculata can be quickly obtained through induction culture and enrichment culture of the calluses, and therefore the problem that the prior art is short of cremastra appendiculata plant resources and medicinal material resources can be effectively solved.

Description

technical field [0001] The invention relates to a method for cultivating rhododendron orchid callus, in particular to a method for cultivating rhododendron orchid callus using rhododendron orchid scape as explants. Background technique [0002] Rhododendron orchid is an orchid herbaceous plant, mainly distributed in Sichuan, Yunnan, Guizhou and other provinces south of the Yangtze River, and its pseudobulb is the medicinal material Shanzi mushroom. At present, more than 60 compounds, such as phenanthrenes, bibenzyls, glycosides, lignans and flavans, have been isolated from the medicinal materials of Shanzi mushroom. Arrowhead mushroom is sweet, slightly pungent, cold, slightly poisonous, enters the Liver and Spleen Meridian, and has the effects of reducing swelling, resolving phlegm, clearing away heat, and detoxifying. It contains colchicine and its compound after adding dilute ammonia solution Colchicine has anti-tumor effects similar to vinblastine, and is widely used in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王跃华陈芳黄鹏马涛唐婕杜媛占洋李航宇石鑫王兰彭轶妮
Owner CHENGDU UNIV
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