Unlock instant, AI-driven research and patent intelligence for your innovation.

Preservation solution and preservation method for cells and application thereof

A preservation method and technology of preservation solution, applied in the field of cell preservation solution, can solve the problem of difficult preservation of cells for a long time, and achieve the effect of stabilizing pH value and resisting pollution

Inactive Publication Date: 2019-05-03
BEIJING USCI MEDICAL LAB CO LTD
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to provide a cell preservation solution, preservation method and application thereof, to solve the problem that cells are difficult to preserve for a long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preservation solution and preservation method for cells and application thereof
  • Preservation solution and preservation method for cells and application thereof
  • Preservation solution and preservation method for cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] As mentioned in the background technology, although the formulation of the preservative in the existing method contains components such as osmotic pressure maintenance agent, mucus digestion component and pH buffering agent, the component of cell fixative is also included in the existing method. The method will lead to the loss of cell activity in an earlier time after the cell shape is fixed, which cannot meet the requirements for cell activity in subsequent experiments.

[0048] In order to verify the effect of the method of the present application and the existing method on the cell activity, this example uses the existing method and the method of the present application to preserve the cells and perform activity detection. details as follows:

[0049] Specifically, in the existing preservatives, the activity of cells presents a pattern of rapid decline within a few hours of taking the sample. In this embodiment, the activity of the cells is detected by the CCK8 met...

Embodiment 2

[0063] Nine (numbered 1-9) were randomly selected from pregnant women aged between 25 and 30 years old, and each sample was preserved by using the original method (N) and the new method (Y) of the present invention respectively. Under different time points and different temperature conditions, the CCK8 method was used to detect the cell activity at multiple points, and the results were compared and analyzed.

[0064] The specific process is as follows:

[0065] 1. Cervical sampling

[0066] 1. Put in the vaginal speculum and wipe the surface of the cervix with a cotton swab to remove mucus and secretions.

[0067] 2. Insert a cervical brush into the cervical canal to match the external os of the cervix.

[0068] 3. Keep a certain pressure on the cervix and cervix, slightly rotate 360° to make full contact (do not turn back and forth), and rotate for 3-5 weeks.

[0069] 4. When the cervical bleeding is obvious during the sampling process, it should be stopped immediately.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preservation solution and a preservation method for cells and an application thereof. The cell preservation solution comprises a DMEM high-glucose culture medium, an antibiotic and an HEPES solution. According to the invention, through discarding of cell-fixation liquid components easily changing cell structures and a pH buffer agent incapable of meeting the concentrationdemand of CO2, and by selection of the cell preservation solution containing the above-mentioned components, components like salt ions and amino acid required for growth and surviving of the cells areprovided; a proper growth and surviving environment is provided for the cells to the greatest possible extent; meanwhile, a stable pH value and the ability of resisting pollution are provided for thecells; the activity of the cells to enable the cells to be in a normal state is maintained to the greatest extent; meanwhile, the cells can be guaranteed to still maintain activity to meet the requirements of subsequent experiments after a long period of time.

Description

technical field [0001] The invention relates to the field of cell preservation and transportation, in particular to a cell preservation solution, a preservation method and its application. Background technique [0002] Cells are an important object in life science research. Due to their high requirements on temperature and environment, living cells have been unable to carry out long-term and long-distance transportation, which has a negative impact on the development of research. There are two main difficulties in the way of cell transport: 1) non-frozen transport. Prone to media surge, resulting in mechanical damage to cells. In order to avoid the surge of the culture solution, it is necessary to fill the culture bottle with the culture solution for safe transportation, and the cost will increase like this. 2) Freeze shipping. Cell stability can be maintained, but the recovery process will cause loss of cell activity, time loss, increase in labor costs and reagent costs,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
Inventor 李红蕊王燕王建伟伍启熹刘倩刘珂弟唐宇
Owner BEIJING USCI MEDICAL LAB CO LTD