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Detection and analysis method of chlorothalonil residues in aquatic animal tissues

An aquatic animal, chlorothalonil technology, applied in the field of detection, can solve the problem of high detection limit, improve stability and recovery, improve detection sensitivity and accuracy, and optimize the effect of chromatography

Active Publication Date: 2020-09-08
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection limit is still high

Method used

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  • Detection and analysis method of chlorothalonil residues in aquatic animal tissues
  • Detection and analysis method of chlorothalonil residues in aquatic animal tissues
  • Detection and analysis method of chlorothalonil residues in aquatic animal tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1: Fish tissue

[0082] From the fish caught in the sampling waters (carp is selected in this embodiment), take its fish tissue, weigh 5.0g of fish sample and 10mL of protease into polypropylene centrifuge tube 1, adjust the pH to 7.5, and use 300W power ultrasonic After 30 minutes, vortex for 1 minute and let stand for 30 minutes.

[0083] Add 10mL acetic acid solution with a volume fraction of 1% acetonitrile to a 50mL centrifuge tube, preheat for 5min, keep the extraction temperature at 70°C, and extract steadily for 5min; vortex for 1min, add 0.5g NaAc, 2g anhydrous MgSO 4 , vortex for 1 min, shake on a shaker for 15 min, centrifuge at 5000 rpm for 5 min, take out most of the extract with a syringe, and after taking out the extract, add 1% acetic acid acetonitrile solution to centrifuge tube 1 again, preheat for 5 min, and keep extracting Temperature 70°C, steady extraction for 5 minutes; vortex for 1 minute, shake for 15 minutes, centrifuge at 5000rpm f...

Embodiment 2-6

[0093] Compared with Example 1, the only difference is that 10mL acetic acid volume fraction of 1% acetonitrile solution is replaced by acetic acid volume fraction of 0% acetonitrile solution, acetic acid volume fraction of 2% acetonitrile solution, acetic acid volume fraction of 3% acetonitrile solution, the volume fraction of acetic acid is 4% acetonitrile solution, and the volume fraction of acetic acid is 5% acetonitrile solution.

[0094] The detection limit of Examples 2-6 is 0.012 μg / L.

Embodiment 7

[0096] Compared with Example 1, the only difference is that "0.5g NaAc, 2g anhydrous MgSO 4 " was replaced with "1g NaAc, 1.5g anhydrous MgSO 4 ".

[0097] In this example, the detection limit is 0.012 μg / L.

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Abstract

The invention discloses a method for detecting and analyzing chlorothalonil residues in aquatic animal tissue, and belongs to the technical field of detection. The method includes the following steps:1) performing extraction on chlorothalonil in aquatic animal tissue; and 2) performing detection on the chlorothalonil in the aquatic animal tissue. Optimization can be performed on sample pretreatment, chromatographic conditions and a detection method on the basis of accomplishing the chlorothalonil extraction in the aquatic animal tissue, so that the method is high in processing efficiency, fast in detection and high in sensitivity, and the chlorothalonil in the aquatic animal tissue can be accurately quantified, and therefore, reliable analysis means can be provided for comprehensive census of the content level and distribution characteristics of the chlorothalonil in the aquatic animal tissue.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection and analysis method for chlorothalonil residues in aquatic animal tissues. Background technique [0002] The chemical name of chlorothalonil (chlorothalonil) is tetrachloroisophthalonitrile, which is a non-systemic, broad-spectrum, high-efficiency protective fungicide, which can prevent and control fungal diseases of various crops. Its bactericidal mechanism is to interact with the glyceraldehyde phosphate dehydrogenase in the fungal cells, combine with the cysteine-containing protein in the dehydrogenase body, inactivate the dehydrogenase, destroy the metabolism of the fungal cells, and lead to the death of the fungus . [0003] Chlorothalonil has been used in world agricultural production for more than 50 years since its production. Due to extensive and long-term use, chlorothalonil residues have been detected in crops and natural ecological environments, which p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 姚晶晶李海普林惠菊杨兆光李跃屠焓钰
Owner CENT SOUTH UNIV
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