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Application of heat shock protein hspa4 in tumor metastasis prediction, prognosis evaluation and treatment

A heat shock protein, tumor metastasis technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of unclear mechanism and association

Active Publication Date: 2020-10-27
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism and association of HSPA4 involved in tumorigenesis and development are still unclear, and its correlation with tumorigenesis and development needs to be further elucidated
[0010] At present, there are no research reports on the relationship between heat shock protein HSPA4 and tumor metastasis, prognosis and treatment at home and abroad.

Method used

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  • Application of heat shock protein hspa4 in tumor metastasis prediction, prognosis evaluation and treatment
  • Application of heat shock protein hspa4 in tumor metastasis prediction, prognosis evaluation and treatment
  • Application of heat shock protein hspa4 in tumor metastasis prediction, prognosis evaluation and treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1: the preparation of detection kit

[0110] The detection kit is prepared according to the following composition, and the kit is suitable for detecting the expression of HSPA4 in biological samples by immunohistochemical method:

[0111] Reagent a: blocking solution, 1 bottle of 10% goat serum (purchased from Beijing Zhongshan Jinqiao Company, product number ZLI-9022), ready-to-use;

[0112] Reagent b: 1 rabbit anti-human or mouse HSPA4 monoclonal antibody (purchased from abcam company in the United States, product number ab185962), 200×concentrated solution;

[0113] Reagent c: 1 bottle of HRP-labeled goat anti-rabbit secondary antibody (purchased from Beijing Zhongshan Jinqiao Company, Cat. No. ZB-2301), ready-to-use;

[0114] Reagent d: DAB substrate buffer solution and chromogenic solution (purchased from Beijing Zhongshan Jinqiao Company, product number ZLI-9019) each 1 tube, 20×concentrated solution;

[0115] As well as containers containing the abov...

Embodiment 2

[0116] Example 2: Expression of HSPA4 in human tumor tissue

[0117] Human tumor samples (breast cancer, lung cancer, pancreatic cancer, and colon cancer) were selected, and the controls were paracancerous tissues of the respective tumors. The above tumor samples and controls were obtained from Shanghai Changhai Hospital. TRIzol (Invitrogen, USA) was used to extract tissue total RNA, and qRT-PCR detection was completed on a LightCycler1.5 (Roche Company) real-time quantitative PCR instrument.

[0118] HSPA4 quantitative PCR primers:

[0119] 5'-GCATCGAGACTATCGCTAATGAG-3' (upstream, SEQ ID NO: 5);

[0120] 5'-TGCAAGGTTAGATTTTTCTGCCT-3' (downstream, SEQ ID NO: 6).

[0121] GAPDH quantitative PCR primers:

[0122] 5'-GGAGCGAGATCCCTCCAAAAT-3' (upstream, SEQ ID NO: 7);

[0123] 5'-GGCTGTTGTCATACTTCTCATGG-3' (downstream, SEQ ID NO: 8).

[0124] Relative quantification of miRNA using 2 -ΔΔCt (GAPDH is an internal reference) [Livak, KJ. ​​et al., Analysis of relative gene expres...

Embodiment 3

[0126] Example 3: Correlation between HSPA4 expression in breast cancer tissue and clinical factors of breast cancer patients

[0127] A total of 233 female breast cancer patients were selected from 2004 to 2005 (tissue sections were obtained from Tianjin Cancer Hospital). The tumor types were all invasive ductal carcinoma (IDC). The patients did not receive chemotherapy and radiotherapy before surgery (see Table 1 for patient information). . All patients signed the informed consent.

[0128] Using the immunohistochemical method, the kit in Example 1 was used to stain the expression of HSPA4 protein in the 233 cases of breast cancer tumor tissues ( figure 2 A), Analysis of the correlation between HSPA4 expression and clinical factors in breast cancer patients. P-values ​​use Chi-square tests in SPSS 17.0.

[0129] Scoring criteria for immunohistochemistry: Scoring was performed independently by two experienced clinical pathologists, and the average value was taken. The ex...

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Abstract

The invention relates to application of heat shock protein HSPA4 in tumor transfer prediction, prognosis evaluation and treatment. Concretely, substances of the heat shock protein HSPA4 for preparation of a prediction object are detected. The heat shock protein HSPA4 and / or genetic inhibitors thereof are applied to medicament for tumor treatment or alleviation or tumor transfer and / or tumour prognosis, as well as corresponding products and medicament combinations. The application has certain clinical prospects.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to the correlation and application of heat shock protein HSPA4 with tumor metastasis prediction, prognosis evaluation and treatment. Background technique [0002] With population growth, aging and social and economic development, malignant tumors are increasingly becoming a major global health problem, which has a great impact on economic and social development and people's health. Therefore, the prevention and control of tumors has become the focus of global health prevention and control. According to the latest data released by the authoritative journal of oncology "Journal of Clinical Oncology" (CA Cancer J Clin), in 2018 the world is expected to increase 18.1 million new cancer cases and 9.6 million deaths due to cancer. The prevalence of cancer in my country is at the upper-middle level in the world (Siegel RL et al., Cancer statistics, 2018. CA Cancer JClin. 2018; 6...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 曹雪涛顾炎刘艳芳
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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