Method and device for collecting volatiles of sunflower disks

Abstract

The invention discloses a method and device for collecting volatiles of sunflower disks. The method comprises the following steps: planting sunflowers at the end of April till the middle and late Julywhen the sunflower flowering period is flourishing, selecting sunflower disks without pest damage for volatile collection; after cutting the sunflower disks, wrapping the injured parts of the plant stems connected to the disks with moist cotton soaked in sterile distilled water immediately to prevent the release of additional volatiles from the injuries; covering the sunflower disks to be testedwith a cylindrical glass cover, and placing the sunflower disks on a flat glass table padded with clean A4 paper to collect the volatiles of the disks; after activating an extraction head after solidphase micro-extraction at 230 DEG C for 30 minutes, inserting the stainless steel round hole into the glass cover through the aperture of the glass cover, continuously enriching the volatiles of the sunflower disks; after the volatiles are enriched for 30 minutes, taking out the extraction head immediately, and performing gas chromatography-gas chromatography-mass spectrometry after thermal analysis at 230 DEG C for 2 minutes. The invention can accurately and scientifically identify volatiles released from sunflower disks.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and in particular relates to a method and a device for collecting volatile matter from sunflower discs. Background technique [0002] sunflower( Helianthus annuus ) is an annual herbaceous plant of the genus Helianthus in the Compositae family. Its plants release relatively less volatiles during the seedling, budding and mature stages, but release more volatiles during the flowering stage and are difficult to collect. In addition, the volatiles released by sunflower during flowering are mainly concentrated in the flower disc. Collecting volatiles from sunflower disks is an important preparatory process for the identification of their chemical components. Therefore, designing a precise and scientific volatile collection device is a key factor in identifying the volatile components of sunflower disks. Solid-phase microextraction (SPME) and sorbent adsorption are two methods for co...

AI Technical Summary

Problems solved by technology

[0003] According to records, the device for collecting volatiles from the sunflower flower disc is mainly to cover the flower disc with a gauze bag. This device has the following disadvantages: 1) the gauze bag has mesh gaps, and the volatiles released by the sunflower flower disc can "escape" from these meshes. Therefore, the volatile components of the sunflower disk detected by this device will be less than the actual release; 2) The gauze bag is generally made of polypropylene (PP), polyethylene (PE), etc., and the taste is relatively Large, if the gauze bag is covered on the faceplate, it will add a lot of impurities. According to the device, the volatile components collected are not actually released by the faceplate itself

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