Method for cooperatively converting bean dregs by virtue of Hermetia illucens and microorganisms

A technology of bright spot flat-horned soldier flies and microorganisms, which is applied in the field of biotechnology to achieve the effects of improving efficiency, improving material reduction rate, and improving biotransformation rate

Inactive Publication Date: 2019-05-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function in lower animals such as insects has not yet been reported. The applicant discovered for the first time that the soldier fly and Lactobacillus brucei provided by the present invention can be used in combination to transform bean dregs, and at the same time promote the growth of soldier flies and improve the The role of soldier fly nutritional value

Method used

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  • Method for cooperatively converting bean dregs by virtue of Hermetia illucens and microorganisms
  • Method for cooperatively converting bean dregs by virtue of Hermetia illucens and microorganisms
  • Method for cooperatively converting bean dregs by virtue of Hermetia illucens and microorganisms

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Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0023] The 6-day-old larvae are the Wuhan strain of the bright-spotted flat-horned soldier fly, (Fen Zhou, Jeffery K.Tomberlin, Longyu Zheng, Ziniu Yu, and Jibin Zhang, Developmental and Waste Reduction Plasticity of Three Black Soldier Fly Strains (Diptera: Stratiomyidae) Raised on Different Livestock Manures.Journal of Medical Entomology, 2013,50(6):1224-1230.), about 3.2mg / head;

[0024] The okara is purchased from the market and is a by-product of tofu and soymilk production, with a water content of 81.3% and a pH of 6.1.

[0025] The experiment was carried out in a soldier fly transformation container (28cm×28cm×12cm), and the temperature of the greenhouse was controlled at 27°C and the relative humidity of the environment was 70%;

[0026] In the present invention, whether it is to inoculate bacterial strains or inoculate larvae, it is calculated based on the wet weight of the material.

Embodiment 1

[0028] A method for jointly transforming bean dregs with light-spotted soldier flies and microorganisms, comprising the steps of:

[0029] Inoculate 1% (v / w) Lactobacillus Brucella bacterium liquid in bean dregs, then inoculate soldier fly larvae (2000 heads / kg bean dregs) raised to 6 days old with artificial feed into the mixture, and let them freely Feed, and treat that 1% of the larvae begin to pre-pupa, and the worm material is separated, and the effective bacterial concentration of the described Lactobacillus Brucella is 10 8 cfu / g.

[0030] The said Lactobacillus buchneri was screened from the fermented wormwood, Gram-positive rod-shaped non-spores, and the contact enzyme negative and paper chromatography test showed that it produces lactic acid. This strain was sent to China Typical Preserved by the Culture Collection Center, classification name: Lactobacillus buchneri L3-9, deposit number: CCTCC NO: M2018511, address: Wuhan University, Wuhan, China. The said Lactobac...

Embodiment 4

[0041] Each parameter analysis and comparison result in embodiment 1-3.

[0042] Table 1 Comparison of larval survival rate, transformation time and biomass

[0043]

[0044] Note: Mean ± standard deviation, n=3, different letters in the same row of data indicate significant differences (P0.05), the same as the following table.

[0045] The larval survival rate (98.0±0.3%) of embodiment 1 is significantly higher than that of embodiment 2 (95.4±0.5%) and implementation 3 (94.02±0.3%), but there is no significant difference between embodiment 2 and embodiment 3. The conversion time of Example 1 (16.1±0.1d) was significantly shorter than that of Example 2 (17.7±0.2d) and Example 3 (18.33±0.1d). The larval biomass of embodiment 1 (146.36 ± 1.79g fresh worm and 34.70 ± 0.92g dry worm) is significantly higher than embodiment 2 (126.3 ± 1.1g fresh worm and 25.2 ± 0.5g dry worm) and embodiment 3 (118.51 ± 0.92g dry worm) 1.23g fresh worms and 19.74 ± 0.81g dry worms, embodiment 2...

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Abstract

The invention belongs to the biological technology field and particularly discloses a method for cooperatively converting bean dregs by virtue of Hermetia illucens and microorganisms. A lactobacillusbuchneri strain L3-9 is obtained by an applicant through screening, the strain and Hermetia illucens can be used for converting the bean dregs. According to the conversion method, the conversion rateof larvae can be increased, the reduction rate of materials can be increased, the feed conversion coefficient can be decreased, the calendar period of the larvae can be shortened, the accumulation amounts of crude proteins and crude fat can be increased, the constitutions of fatty acid and amino acid are accordant with the human use standard specified by WHO/FAO/UNU, and heavy metal ions and anti-nutritional factors are accordant with the safety standard. Therefore, the bean dregs are cooperatively converted by Hermetia illucens and lactobacillus buchneri, so that the cooperative conversion efficiency is improved, and the nutritional value of Hermetia illucens is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for jointly transforming bean dregs by light-spotted soldier flies and microorganisms. Combining transformation of Soldier Fly glabrata and Lactobacillus brunii for bean dregs not only improved the combined transformation efficiency, but also improved the nutritional value of Soldier Fly glabrata. technical background [0002] China is the birthplace of tofu production, has a long history of tofu production, and the production and sales of tofu are relatively large. In addition to the consumption of soybeans in the traditional form of tofu, a new consumption method - soybean milk is welcomed by consumers. For example, in Argentina, Brazil, and the United States, the production volume of soybean milk is very large. Okara is a by-product in the process of producing soy milk or tofu. For example, 1.2kg of okara will be produced for every 1kg of soymilk or tofu pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/37A23K10/18
CPCY02P60/87
Inventor 张吉斌肖小朋苏马柔蔡珉敏郑龙玉喻子牛
Owner HUAZHONG AGRI UNIV
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