Production method of paraffin section for observing flower bud differentiation process

A technology of paraffin sectioning and flower bud differentiation, applied in the field of plant sample observation, can solve the problems of incomplete sectioning, high toxicity, and difficult production, and achieve the effects of reducing brittleness, ensuring accuracy, and high accuracy

Inactive Publication Date: 2019-05-10
SHANXI ACAD OF AGRI SCI GARDENING RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the above problems, and the purpose is to provide a method for making paraffin slices for observing the flower bud differentiation process, which can solve the technical problems of the prior art such as high toxicity or incomplete slices, limited sampling time / difficult production, etc.

Method used

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  • Production method of paraffin section for observing flower bud differentiation process
  • Production method of paraffin section for observing flower bud differentiation process
  • Production method of paraffin section for observing flower bud differentiation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Microstructure of Hemerocallis shoot tip in vegetative stage

[0039] Take material and fix the air: After dissecting the leaves basally grown on the rhizome of Hemerocallis basalis, take the central point of the stem tip of Hemerocallis basalis and the basal line of the leaves as the axis, and immediately cut carefully 5mm (parallel to the basal line of the leaves) × 3mm ( The central tissue of the Hemerocallis shoot tip (perpendicular to the basal line of the leaf) × 5mm (height), is immersed in a 10ml test tube (with a cover) filled with FPA fixative solution for fixed preservation, and the volume ratio of the material fixative solution is 1:40. Subsequently, put the test tube into a vacuum desiccator, open the test tube cover, and vacuumize for 1-2 hours until all the samples sink to the bottom.

[0040] Dehydration and transparency: After the fixation is completed, pour out the fixative in the test tube and place it on the test tube rack. Sequentially r...

Embodiment 2

[0046] Example 2: Microstructure of Hemerocallis flower buds in flower bud differentiation stage

[0047] In the fixed air extraction stage of obtaining materials in embodiment 1: after dissecting the blades that were basal on the rhizome of Hemerocallis, take the basal line of the central point of the Hemerocallis stem tip and the blade as the axis, and immediately carefully cut 5mm (parallel to the base of the blade) Raw line) × 3mm (perpendicular to the basal line of the leaf) × 5mm (height), the central tissue of the Hemerocallis shoot tip, immersed in a 10ml test tube (with a cover) filled with FPA fixative solution for fixed preservation, the volume ratio of the material fixative solution is 1 :40. Subsequently, put the test tube into a vacuum desiccator, open the test tube cover, and vacuumize for 1-2 hours until all the samples sink to the bottom.

[0048] In the dehydration transparent stage of Example 1: after the fixation is completed, the fixative in the test tube...

Embodiment 3

[0051] Example 3: Microstructure of Hemerocallis flower buds at the stage of small flower bud formation

[0052] In the fixed air extraction stage of obtaining materials in embodiment 1: after dissecting the blades that were basal on the rhizome of Hemerocallis, take the basal line of the central point of the Hemerocallis stem tip and the blade as the axis, and immediately carefully cut 5mm (parallel to the base of the blade) Raw line) × 3mm (perpendicular to the basal line of the leaf) × 5mm (height), the central tissue of the Hemerocallis shoot tip, immersed in a 10ml test tube (with a cover) filled with FPA fixative solution for fixed preservation, the volume ratio of the material fixative solution is 1 :40. Subsequently, put the test tube into a vacuum desiccator, open the test tube cover, and vacuumize for 1-2 hours until all the samples sink to the bottom.

[0053] In the dehydration transparent stage of Example 1: after the fixation is completed, the fixative in the te...

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Abstract

The invention relates to a production method of a paraffin section for observing a flower bud differentiation process. The method comprises the following steps: plant sample material taking and fixing, dehydrating and transparent, waxing and embedding, paraffin block correcting and slicing, adhesive tape unfolding, and dyeing and sealing; the step of plant sample material taking and fixing comprises cutting a plant stem tip central tissue, wherein the plant stem tip central tissue is cut with a stem tip central point and a base line of a blade as an axis, wherein the cutting width of the plantstem tip central tissue in the direction parallel to the base line of the blade is 4 to 6mm, the cutting width in the direction vertical to the base line of the blade is 2 to 4mm, and the cutting width in the height direction is 5 to 7mm. According to the production method of the paraffin section for observing the flower bud differentiation process provided by the invention, a special material taking method is adopted, the growth state of the plant stem tip can be reflected truly and accurately, and a cytological perspective is provided for observing the plant flower bud differentiation process; compared with a traditional paraffin section fixing method, the fixing method disclosed by the invention reduces the situation that young and tender materials become brittle after being fixed. The method of the invention ensures the accuracy of observing and measuring the angle of a growth point of the stem tip, is more efficient than the traditional paraffin section, and the obtained sectionhas higher accuracy.

Description

technical field [0001] The invention relates to the technical field of plant sample observation, and more particularly, relates to a method for making paraffin sections for observing plant flower bud differentiation processes. Background technique [0002] Flowering is an important stage in the life history of higher plants. In recent years, with the analysis of the molecular mechanism of flower formation in the model plant Arabidopsis thaliana, people have gained a deeper understanding of the flower formation and development mechanism of annual herbaceous plants; however, there is a huge gap between the reproductive habits of perennial herbaceous plants and annual herbaceous plants. Although some progress has been made in the study of floral transition in Arabidopsis, so far, our understanding of the molecular mechanism of flowering in perennial herbaceous plants has yet to be further explored. [0003] In the related research on flower formation and development of perenni...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
Inventor 李志娟付宝春王云山李素清
Owner SHANXI ACAD OF AGRI SCI GARDENING RES INST
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