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Composition as well as kit, biochip and application thereof

A biochip and composition technology, applied in the field of tumor molecular biology, can solve the problems of different effects, failure to consider the response diversity of individual treatments, and inability to accurately predict the lymph node metastasis of oral squamous cell carcinoma.

Active Publication Date: 2019-05-10
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

[0004] So far, although some molecular markers for predicting the metastasis of oral squamous cell carcinoma have been discovered, due to individual differences, the molecular markers discovered so far cannot accurately predict the lymph node metastasis of oral squamous cell carcinoma, and the gold standard for diagnosis is still Histopathology-based tissue biopsy techniques
However, histopathology can and can only roughly predict the prognosis of patients with oral squamous cell carcinoma, and it does not take into account the individual response to treatment and the diversity of prognosis, so the predictive value varies from low to high

Method used

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  • Composition as well as kit, biochip and application thereof
  • Composition as well as kit, biochip and application thereof
  • Composition as well as kit, biochip and application thereof

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Experimental program
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Effect test

Embodiment 1

[0049] The screening of embodiment 1 composition

[0050] Through the analysis of the protein profiles related to lymph node metastasis of oral squamous cell carcinoma in the literature, and the establishment of protein screening criteria for lymph node metastasis of oral squamous cell carcinoma, related proteins were included in candidate molecular markers. Among them, the criteria for protein screening are: ① Proteins with different expression abundance in OSCC lymph node metastasis and non-metastatic OSCC (P<0.05); ② Proteins with clinically significant differences in prognosis of patients (P<0.05); ③In the protein database, the proteins positively expressed in tumor tissues, and ④The proteins that have been verified in previous studies and are related to the metastasis of oral squamous cell carcinoma. The expression levels of related proteins in 58 metastatic and non-metastatic tumor tissues of oral squamous cell carcinoma were analyzed by immunohistochemical technique, an...

Embodiment 2

[0086] Example 2 Verification of the prediction model

[0087] In order to ensure the accuracy of the prediction of OSCC metastasis, the prediction model was further verified with 101 OSCC tissue microarrays. The tissue chips of 101 cases of oral squamous cell carcinoma were obtained from tumor tissues excised from patients with oral squamous cell carcinoma in the Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, without radiation therapy and chemotherapy before surgery. We statistically analyzed the histopathological grades of 101 metastatic and non-metastatic tumor tissues in the tissue chip, and used immunohistochemical methods (the same method as in Example 1) to analyze the protein expression levels of CAD and SOD2 in 101 metastatic and non-metastatic tumor tissues To detect and analyze. The results of immunohistochemical analysis of CAD and SOD2 were as follows: Figure 9 As shown, the statistical chart of the results of immunohisto...

Embodiment 3

[0089] Example 3 Prediction of Survival Rate and Disease-Free Survival Rate of Oral Squamous Cell Carcinoma Patients by Prediction Model

[0090] In addition, statistical analysis was carried out on the survival information of 58 patients with metastatic and non-metastatic oral squamous cell carcinoma, and the survival rate statistics were carried out. The experimental results are as follows: Figure 5 shown. Depend on Figure 5 It can be seen that the three difference variables included in the prediction model, CAD, SOD2, histopathological grade and lymph node metastasis, are significantly correlated with the overall survival rate and disease-free survival rate.

[0091] The effect of CAD, SOD2, histopathological grade and lymph node metastasis on the overall survival rate and disease-free survival rate in the metastatic and non-metastatic tumor tissues of 101 patients with the above-mentioned tissue chip was verified. The results are as follows: Figure 11 shown. From F...

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Abstract

The invention discloses a composition for oral squamous cell carcinoma lymphatic metastasis prediction as well as a kit, a biochip and an application thereof. The composition as well as the kit and the biochip thereof respectively comprise a reagent capable of detecting CAD protein expression level and a reagent capable of detecting SOD2 protein expression level. The composition can detect contents of CAD protein and SOD2 protein in a patient suffering from oral squamous cell carcinoma, and then oral squamous cell carcinoma lymphatic metastasis probability is predicted according to the contents of the CAD protein and the SOD2 protein as well as histopathological grading. Prediction performed by utilizing the composition disclosed by the invention as well as the histopathological grading ismore accurate, an alarm can be sounded for the patient in advance, and delayed treatment is avoided, so that the composition disclosed by the invention has a broad application prospect.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to a composition and its kit, biochip and application. Background technique [0002] Oral cancer, mainly oral squamous cell carcinoma, is the sixth most common tumor in the world. According to the International Agency for Research on Cancer, 350,000 people were diagnosed with oral squamous cell carcinoma and 170,000 people died of oral cancer in 2018. Oral squamous cell carcinoma mainly metastasizes through lymph nodes, and distant metastasis rarely occurs. Lymph node metastasis may be the main reason for the poor prognosis of oral squamous cell carcinoma. The 5-year survival rate of patients with cervical lymph node metastasis is only 20-45%, while that of patients without metastasis is 90%. Elective neck dissection can improve patient survival, but there is still controversy about how to define clinically and radiologically undetectable early lymph node metastases. Many ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531
Inventor 陈万涛于玢玢
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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