A fluorescence microscopic counting method for detecting the number of bacteria in water

Abstract

The invention belongs to the technical field of water body bacterium counting. The invention discloses a fluorescence microscopic counting method for detecting the quantity of bacteria in a water body. The fluorescence microscopic counting system for detecting the quantity of the bacteria in the water body comprises a light source module, an image acquisition module, a central control module, a focusing module, a color filtering module, an amplification module, an image processing module, a marking module and a display module. According to the invention, an image matching correlation functionis adopted by the focusing module for focusing, so that the problem that a traditional definition function cannot be directly used for fluorescent images is solved, and the focusing speed and the stability are effectively improved; meanwhile, recording a low-resolution image Y of the real image X by using a low-resolution recording camera of the fluorescence microscope through an image processingmodule, performing interpolation processing on the low-resolution image Y, and then reconstructing the real image through h; the image resolution is greatly improved, the image is clearer, and the number of bacteria is conveniently calculated.

Description

technical field [0001] The invention belongs to the technical field of water body bacteria counting, in particular to a fluorescence microscopic counting method for detecting the number of water body bacteria. Background technique [0002] Aquatic bacteria are a class of ultramicro aquatic organisms that grow in water bodies. It is an important member of the aquatic biome. The shape ranges from a few tenths of a micron to tens of microns. There are many types, including cocci, bacilli, vibrios, and spirochetes. According to the habitat environment, it can be divided into two categories: benthic bacteria and planktonic bacteria. The structure is simple, with cell wall, plasma membrane, cytoplasm, nucleus, etc. It is at a low level in biological evolution. Breeding fast, split once every 20-30min. widely distributed. The quantity is closely related to the fertility of the water body and the quality of the water. Aquatic bacteria are the main link to complete the water f...

AI Technical Summary

Problems solved by technology

However, the current fluorescence microscopic counting process for detecting bacteria in water bodies has slow focusing speed and poor stability; at the same time, the collected images have low resolution and poor clarity

[0003] To sum up, the problems existing in the prior art are: the focusing speed and poor stability of the existing fluorescent microscopic counting process for detecting the number of bacteria in the water body; at the same time, the collected images have low resolution and poor clarity.

The traditional SOFI algorithm requires hundreds of images, which reduces the speed of image processing. The signal-to-noise ratio of the original image determines the number of original images required for algorithm processing; the image field area of ​​the objective lens of the traditional microscope is small, and the image field is seriously curved ; At the same time, the objective lens adopts a limited image distance, resulting in large chromatic aberration, which requires multiple corrections

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