A process for producing purified phycoerythrin

A technology for purifying phycoerythrin and process, which is applied in the field of producing purified phycoerythrin, can solve the problems of large difference in influence, no uniformity, and cannot be copied by other algae, etc., and achieves the effects of high biological activity, promotion of accumulation, and improvement of photosynthesis.

Active Publication Date: 2020-06-09
江西抚州新兴化工有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, some exogenous phytohormones also have a greater impact on the growth of algae, but there are many types of phytohormones, and the effects of different phytohormones on different algae are quite different. There is no unified experience for reference, and it cannot be based on other algae. copy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A process for producing purified phycoerythrin
  • A process for producing purified phycoerythrin
  • A process for producing purified phycoerythrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A process for producing purified phycoerythrin, comprising the steps of:

[0029] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0030] Put the Porphyridium algae seed solution into the reaction tank containing artificially synthesized culture solution according to the inoculation amount of 10%, control the light intensity to 5000Lux, the air flow rate to 0.5vvm, cultivate at 25°C, and the light-to-dark ratio is 14:10 , cultivated for 3 days, then added 0.02 mg / L of brassinolide and 0.3 g / L of bionitrogen, and continued to cultivate for 5 days to obtain algae liquid;

[0031] The components of the culture solution are: sodium chloride 12g / L, sodium nitrate 1g / L, sodium carbonate 0...

Embodiment 2

[0034] A process for producing purified phycoerythrin, comprising the steps of:

[0035] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0036]Put the Porphyridium algae seed liquid into the reaction tank containing artificially synthesized culture liquid according to the inoculum amount of 10%, control the light intensity to 6000Lux, the air flow rate to 0.3vvm, cultivate at 22°C, and the light-to-dark ratio is 14:10 , cultivated for 3 days, then added 0.01 mg / L of brassinolide and 0.4 g / L of biological nitrogen, and continued to cultivate for 4 days to obtain algae liquid;

[0037] The components of the culture solution are: sodium chloride 12g / L, sodium nitrate 1g / L, sodium carbonate 0...

Embodiment 3

[0040] A process for producing purified phycoerythrin, comprising the steps of:

[0041] Inoculate the Porphyridium algae cultivated to the logarithmic growth phase into the seed tank containing the f / 2 medium, and the inoculation initial density is 2×10 5 cells / ml, light intensity 4000lux, culture at 23°C, light-to-dark ratio of 14:10, air flow rate of 0.4vvm, culture for 2 days, and collect Porphyridium algae seed liquid;

[0042] Put the Porphyridium algae seed solution into the reaction tank containing artificially synthesized culture solution according to the inoculum amount of 10%, control the light intensity to 6000Lux, the air flow rate to 0.5vvm, cultivate at 24°C, and the light-to-dark ratio is 14:10 , cultivated for 3d, then added 0.04mg / L brassinolide and 0.34g / L bionitrogen, and continued to cultivate for 5d, to obtain algae liquid;

[0043] The components of the culture solution are: sodium chloride 12g / L, sodium nitrate 1g / L, sodium carbonate 0.5g / L, borax 0.2g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of algae cultivation, and discloses a process for producing and purifying phycoerythrin, which comprises the following steps: step 1) Porphyridium algae seed liquid, step 2) large-scale cultivation, and step 3) separation and purification. The process of the invention not only increases the content of phycoerythrin in Porphyrococcus, but also separates and purifies the phycoerythrin.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to improving a process for producing purified phycoerythrin. Background technique [0002] Phycoerythrin (phycoerythrin) exists in the pigment protein of red algae, cyanobacteria, and cryptodininosaurs, and the ring-opened tetrapyrrole phycoerythrin is covalently bonded to the protein as the pigment part. The prosthetic group is a chain composed of pyrrole rings, the molecule does not contain metal, and is combined with the protein. Phycoerythrin has strong absorption in the 480-570 band in the visible light region, and can produce strong fluorescence. No matter in terms of phycoerythrin, phycocyanin or other natural protein pigments, they are usually practical and safe, and have a considerable degree of stability to heat, pH, etc., and can be used in food and cosmetics, and can also be used as an immunological It is used as a fluorescent dye for antibody lab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54C07K14/405
Inventor 胡叶飞张菊萍董国强张宏杰金荣培
Owner 江西抚州新兴化工有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap