A kind of cell carrier particle aggregate and preparation method thereof

A technology of carrier particles and aggregates, applied in general culture methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve the problems of polluting the surrounding environment, difficulty in use, time-consuming and laborious, etc.

Active Publication Date: 2021-08-03
BEIJING CYTONICHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it is a micron-level microbead, there is a lot of static electricity in the dry powder state, so it is very easy to cause the splash of the microcarrier powder during use or transportation, the effective mass is reduced, and the surrounding environment is polluted at the same time.
It is also difficult to preserve powdered microcarriers, and its own nature limits that no matter what packaging it uses, it will cause a lot of unnecessary loss
It is difficult to use, and a lot of measuring equipment is needed as auxiliary tools in many processes such as weighing, which is time-consuming and labor-intensive.

Method used

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  • A kind of cell carrier particle aggregate and preparation method thereof
  • A kind of cell carrier particle aggregate and preparation method thereof
  • A kind of cell carrier particle aggregate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, stamping aggregate molding

[0065] The stamping and gathering forming machine is an automatic stamping and gathering forming machine, and the motor rocker rotates one week to produce a finished product. The stamping and aggregation molding machine is fixed and quantitative by the method of measuring volume.

[0066] First weigh 20 mg of cell carrier particles on a balance, then pour the weighed material into the feeding place of the stamping molding machine, and select an inclined flat punching mold. Adjust the upper punch of the stamping aggregation forming machine, the adjustment range is 0-50mm, and adjust the lower punch of the stamping aggregation molding machine, the adjustment range is 0-50mm. Run the handle of the stamping and gathering molding machine to fill the material, adjust the pressure value, the pressure value can be controlled within the range of 0-200KN, turn on the motor, and shake the rocker of the motor for one week to complete a st...

Embodiment 2

[0077] Embodiment 2, mold forming

[0078] Take a certain amount of powdered cell carrier particles, add deionized water (the amount added is 50 times the weight of the cell microcarriers), and use auxiliary tools to make it evenly and evenly cover the mold. The mold is cylindrical (can also be circular, square, rhombus, triangle, ellipse, polygon and polygon, etc.). A mold containing a certain mass of cell carrier particles (such as Figure 8 (shown) placed in an oven at 60°C and dried continuously for 96 hours to obtain solid and stable aggregates of cell carrier particles with the same unit mass and shape, as shown in Figure 9 shown.

[0079] Put the cell carrier particle aggregate prepared in this example into water, and it can be quickly dispersed into the state of individual cell carrier particles, such as Figure 10 As shown, the results show that there is no change in its shape and physical properties.

[0080] The cell carrier particle aggregates prepared in this...

Embodiment 3

[0083] Embodiment 3, lyophilization and drying molding

[0084] Take a certain amount of powdered microcarriers, add deionized water (the amount added is 100 times the weight of the cell microcarriers), and use auxiliary tools to make them aggregate into regular shaped blocks, such as Figure 11 As shown; put the aggregates into a freezing container, and form a block-shaped frozen body during the low-temperature freezing process at 0 to -196 ° C; put the frozen aggregates into a freeze dryer, freeze-dry for 96 hours, and take them out After that, freeze-dried aggregates were formed, such as Figure 12 shown.

[0085] The cell carrier particle aggregates prepared in this example can be quickly dispersed into the state of individual microcarrier particles, such as Figure 13 As shown, the results show that there is no change in its shape and physical properties.

[0086] The cell carrier particle aggregates prepared in this example have the property of quickly absorbing water...

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Abstract

The invention discloses a cell carrier particle aggregate and a preparation method thereof. The cell carrier particle aggregate is formed by the aggregation of cell carrier particles and has a specific shape; the specific shape includes sheet and block; the cell carrier particle aggregate has excellent rehydration and good dispersibility after rehydration. The interior of the cell carrier particle in the cell carrier particle aggregate of the present invention has porous connectivity, and has great elasticity and anti-extrusion ability. After the aggregate is formed by pressing, it can be quickly dispersed into a separate carrier scaffold by adding a very small amount of liquid carrier particles, without any change in physical and chemical properties; without adding any other ingredients, there is no powder on the surface of the aggregate obtained by the molding method , so that the static electricity can be reduced as much as possible to avoid the electrostatic generation of powder products and cause losses; and it can be sterilized and stored in a single piece in the form of a medicine plate, or the aggregate can be sterilized and stored in multiple pieces in the form of a bottle, and sterilized in an independent package , to facilitate aseptic operation of cell culture.

Description

technical field [0001] The invention relates to a cell carrier particle aggregate and a preparation method thereof. Background technique [0002] Cell carrier particles (generally 1-1000um in diameter) refer to microbeads that can be grown or co-cultured with adherent cells. At present, microcarriers on the market are used, stored and packaged in the form of powder. Because it is a micron-level microbead, it is easy to have a lot of static electricity in the dry powder state, so it is very easy to cause the splash of the microcarrier powder during use or transportation, reduce the effective mass, and pollute the surrounding environment while also polluting itself. Preservation of powdered microcarriers is also relatively difficult, and its nature limits that it will cause a lot of unnecessary loss no matter what packaging is used. It is difficult to use, and a lot of measuring equipment is needed as auxiliary tools in many processes such as weighing, which is time-consumin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/00
CPCB29B11/06C12N5/0075C12N2533/52C12N2533/54C12M25/16B29C41/003B29C41/38B29K2089/00B29K2833/12C12N11/02B29C43/003B29C43/36B29C39/003B29C39/02B29C39/26C08J3/12B29C2043/3615
Inventor 刘伟鄢晓君
Owner BEIJING CYTONICHE BIOTECH CO LTD
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