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A method for Citrobacter freundii to remove cadmium in water and generate nanomaterials

A Freund's citric acid and water removal technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of incomplete removal of heavy metals and difficult removal of heavy metals

Active Publication Date: 2021-05-14
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

This method has the problem of incomplete removal of heavy metals, mainly because the bacteria micelles will disappear due to the reduction of nutrients around the bacteria, and the heavy metals adsorbed by the bacteria micelles will be released into the water again after the disappearance; the complexation process of heavy metals and proteins The chelate will be decomposed by microorganisms, the complex will not exist after decomposition, and the heavy metal will be released into the water again
The present invention aims to provide a new degradation pathway for heavy metals, which converts heavy metals into stable precipitation substances or minerals, so that with the death of microorganisms, the removed heavy metals will not re-enter the water, but in the form of stable precipitation This kind of precipitation can be collected and reused as nanomaterials, and this removal method can also be extended to soil to solve the problem of difficult removal of heavy metals in soil

Method used

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  • A method for Citrobacter freundii to remove cadmium in water and generate nanomaterials
  • A method for Citrobacter freundii to remove cadmium in water and generate nanomaterials
  • A method for Citrobacter freundii to remove cadmium in water and generate nanomaterials

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Embodiment Construction

[0017] 1. Weigh 10g of peptone, 5g of beef extract, 5g of sodium chloride, and 40mg of cadmium chloride, dissolve them in 1000ml of deionized water, add sodium hydroxide to adjust the pH of the solution to neutral, and prepare a solution containing Peptone and beef extract medium with 40 mg / L cadmium (hereinafter referred to as the medium with metals and no phosphates), put it in a high-pressure steam sterilizer and sterilize it at 121°C for 30 minutes, then take it out and place it in a sterile In the operating table, inoculate with Citrobacter after cooling down to room temperature.

[0018] 2. Weigh 10g of peptone, 5g of beef extract, 5g of sodium chloride, 40mg of cadmium chloride, and 116mg of disodium phosphate glyceride pentahydrate (the molar ratio to cadmium is 1:2), and dissolve them in 1000ml of deionized In the water, after adding the pH of sodium hydroxide to regulate the solution to neutrality, prepare the peptone that contains cadmium chloride 40mg / L, disodium p...

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Abstract

The present invention relates to the technical field of microbial cultivation, water body purification, and nanomaterial synthesis, and specifically relates to a method of cultivating Citrobacter freundii to remove heavy metal cadmium in water and produce nanomaterial precipitation in situ, especially to a method of Citrobacter freundii decomposes the nutrients around the culture solution to generate a large amount of acid ions, some acid ions such as carbonate and phosphate react with heavy metal cadmium to form cadmium nano-scale precipitation, so as to achieve the removal of heavy metal cadmium in water At the same time, the corresponding nanometer metal materials are also synthesized.

Description

technical field [0001] The present invention relates to the technical fields of microbial cultivation, water purification, and nanomaterial synthesis, and specifically relates to a method for in-situ removal of heavy metal cadmium in water and precipitation of nanomaterials by cultivating Citrobacter freundii. Citrobacter freundii decomposes the nutrients around the culture medium to generate a large amount of acid radical ions, and some acid radical ions such as carbonate and phosphate react with heavy metal cadmium to form nanoscale precipitation of cadmium, thereby achieving the removal of heavy metal cadmium in water At the same time, the corresponding cadmium nano-metal materials were synthesized. Background technique [0002] Now the heavy metal pollution in water bodies in our country is particularly serious. The pollution of heavy metals in water bodies is led by cadmium pollution. Because heavy metals mostly exist in the form of ions in the liquid phase, this form ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12N1/20C02F3/34C02F101/20C12R1/01
Inventor 王珅郭思瑶韩松刘力宁于杰
Owner NORTHEAST FORESTRY UNIVERSITY
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