37 results about "Citrobacter sp" patented technology

The invention discloses a method for improving the formation of a citrobacter werkmanii biofilm and provides a method for improving the biofilm forming ability of a strain by knocking out an outer membrane protein ompA gene of citrobacter werkmanii. A knockout vector is constructed by using a plasmid pYG4, and an ompA gene is knocked out by using a gene homologous recombination principle, so thatdelta ompA is obtained. The delta ompA character is analyzed through crystal violet staining and other methods, and the result shows that compared with wild citrobacter werkmanii, the biofilm formingability of delta ompA is improved by four times or above, and the drug resistance of delta ompA to isothiazolinone bactericides is also correspondingly improved. According to the ompA knockout strainconstructed by the invention, the formation amount and the drug resistance level of a citrobacter welchii biofilm can be effectively improved, so that the application scenarios and ranges of citrobacter werkmanii for heavy metal ion adsorption, construction of cell protein synthesis factories and the like are broadened.

The invention discloses a degrading bacterium capable of efficiently degrading a malachite green drug and application thereof. The degrading bacterium is a bacterial strain D3 of citrobacter sp., andis preserved in the China Center for Type Culture Collection on March 16, 2018 with a preservation number of CCTCC No. M 20181314. The bacterium has ability of degrading the malachite green drug in afish farming environment. The invention further discloses a preparing, separating and purifying method for the degrading bacterium capable of efficiently degrading the malachite green drug. The degrading bacterium capable of efficiently degrading the malachite green drug can safely, efficiently and quickly degrade the residual malachite green drug in the fish farming environment, and has a very good application prospect in fish ecological environment protection, farming environment drug residue pollution control and food safety guarantee.

The invention belongs to the field of environmental microbiology, and relates to a Citrobacter sp. strain and application thereof. The Citrobacter sp. is named as deodorizing bacteria ZT-S5, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.15120 on December 25, 2017. The Citrobacter sp. strain provided by the invention has high vitality and high adaptability, and can be widely applied to the biological treatment process of hydrogen sulfide and ammonia in water and gas. A biological deodorizing device made of the strain by means of adsorption can resist the impact of waste gas well, and the application of biological deodorization is realized.

The present invention discloses citrobacter sp. for removing H2S gas from gas, and a use thereof. The citrobacter sp. desulfurization heterotrophic bacteria CGMCC No.5285 for removing the H2S gas from the gas has the function of H2S gas removing from the gas. The experimental results show that: with arranging the citrobacter sp. desulfurization heterotrophic bacteria CGMCC No.5285 on a ceramsite filtering material, and filling the resulting material in a bio-trickling tower, the bio-trickling tower can rapidly remove the H2S gas, the mass concentration of the H2S on the H2S outlet is less than 0.03 mg / m<3> after treating so as to meet the factory boundary first class standard in the GB14554-93 emission standards for odor pollutants.

The invention discloses a bacterial strain capable of degrading petroleum hydrocarbon and application thereof, wherein, the bacterial strain is Citrobacter sp. WTS (CGMCC No.3253) highly capable of degrading petroleum hydrocarbon. The bacterial strain, bacterial agent and application method thereof in the invention provide theoretical basis for the bioremediation of oil-polluted soil in China, have great significance to the environmental protection of oil-field areas in China and the remediation of pollution caused by leakage in the oil transportation, and accelerate the progress of bioremediation of oil pollution, therefore, the invention is of great economic significance.

The invention relates to a biological compound degradation agent in an anaerobic condition and application thereof. The biological compound degradation agent comprises Citrobacter sp H5-XJ, the preservation number of which is CGMCC No.13504 and Galactomyces sp H5-ZJ, the preservation number of which is CGMCC No.13378. Both the Citrobacter sp H5-XJ and the Galactomyces sp H5-ZJ have been preserved in CGMCC on 26th, December, 2016. The invention further discloses application of the biological compound degradation agent in the anaerobic condition. The biological compound degradation agent provided by the invention has the advantages that the biological compound degradation agent can degrade high concentration ammonia and nitrogen in the anaerobic condition; no nitrite nitrogen and nitrate nitrogen are accumulated; nitration and denitration are performed synchronously, so that the ammonia and nitrogen degradation efficiency reaches 90% above.

The invention discloses a citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit. By means of the citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit, a pair of primers is designed according to the citrobacter freundii 16S rRNA gene sequence in a GenBank; a fluorescent quantitative PCR reaction condition is optimized, and the citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit is developed. The citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit is used for testing clinical samples simply, conveniently, quickly, sensitively and accurately, and a scientific basis is provided for prevention and treatment of the disease. The studied and designed primers are applied to a fluorescent quantitative PCR rapid test kit, so that citrobacter freundii is quickly tested, and good specificity and sensitivity are achieved.

The invention relates to the technical field of livestock and poultry breeding, in particular to a feed antibacterial additive containing low-temperature glucose oxidase and application of the feed antibacterial additive. The feed additive comprises glucose oxidase and silicon dioxide, and the glucose oxidase is produced from a citrobacter sp. 8-III strain which produces glucose oxidase, has a preservation number of CGMCC NO.17228 adn and has low-temperature enzyme characteristics. When the antibacterial feed additive is used, the antibacterial feed additive is directly added into feed, and the mildew rate of the feed can be reduced when the feed is stored at 23 DEG C. The antibacterial feed additive provided by the invention comprises glucose oxidase and has the advantages of high safetyand no drug resistance; and the feed additive is capable of regulating the balance of intestinal flora, generating an environment beneficial to the growth of beneficial bacteria, promoting the growthof animals, improving the feed conversion efficiency and reducing the incidence rate of diseases by inhibiting the reproduction of harmful microorganisms in animal intestinal tracts, can effectively replace antibiotics, conforms to the development direction of healthy breeding and has a good application prospect in the field of feeds.

The invention discloses a citrobacter welchii tpiA gene knockout mutant strain and application thereof, and belongs to the field of gene engineering. The citrobacter welchii tpiA gene knockout mutant strain is obtained by completely knocking out coding genes from the first site to the 768th site of a citrobacter welchii wild strain tpiA gene, and the coding gene sequence from the first site to the 768th site of the tpiA gene is as shown in SEQ ID NO.1. The citrobacter welchii GDFMZ BF-8 delta tpiA has a preservation number of GDMCC 61942, and experiments prove that the mutant strain has the capability of reducing the biofilm formation amount of the citrobacter welchii. Meanwhile, the invention further provides a method for reducing the biofilm formation amount of the citrobacter welchii. The invention also proves that the tpiA gene is an action site of imatinib mesylate and can be used in a target site controlled by a biofilm.

The invention provides a heavy-metal-copper-resistant bacterial strain and application thereof. The classification and name of the heavy-metal-copper-resistant bacterial strain is Citrobacter sp.JPG1, and has the preservation register number of CGMCC (China General Microbiological Culture Collection Center) No.13480 in CGMCC. The strain is obtained through separation, screening, identification and mutagenesis. The bacteria can endure and adsorb heavy metal copper, and can be used for microbial treatment of heavy-metal-copper-containing wastewater.

The invention discloses an application of citrobacter freundii in efficient degradation of DMAc (dimethylacetamide). Belongs to the technical field of microorganisms. Aiming at the defects of a traditional biological treatment method, the citrobacter freundii can efficiently degrade DMAc through screening, and the citrobacter freundii has the advantages of being easy to operate, low in cost, environmentally friendly and the like.

The invention relates to specific nucleotides for 017 and 039 serotypes of citrobacter and an application of the specific nucleotides. The nucleotides include at least one of nucleotides represented as SEQ ID NO: 1-4, the nucleotides can be used for preparing a PCR (polymerase chain reaction) kit for detecting the citrobacter. The citrobacter can be sampled from crude extract of culture of the digestive tract, urine, blood and wounds and can also be obtained through separation in feces environment of humans and animals. According to the specific nucleotides for the 017 and 039 serotypes of the citrobacter and the PCR kit containing the nucleotides, a preparation method of the PCR kit is simple, the detection period is short, the speed is high, the operability is high, industrial production is facilitated, and the detection cost is relatively lower; the accuracy is high; the sensitivity is high.

The invention relates to aboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate, and an application of theaboriginal efficient denitrification strains of acinetobacter baylyi A9 and citrobacter youngae A13 from rare earth mineral leaching space leachate. The strains respectively belong to Acinetobacter baylyi and Citrobacter youngae, which are transferred to the China Center for Type Culture Collection for preservation on January 10th, 2020, and corresponding preservation numbers are CCTCC NO:M 2020031 and CCTCC NO:M 2020030. The two strains are both from the rare earth mineral leaching space leachate, are both heterotrophic nitrification-aerobic denitrification denitrification strains, can efficiently remove ammonia nitrogen in the rare earth mineral leaching space leachate through an organic carbon source under the aerobic condition, besides, also have better removing effects on nitrate andnitrite, and have the advantages of being high in adaptability, high in denitrification efficiency and the like.

The invention relates to a group of primers for assaying citrobacter rodentium and a kit thereof, which belong to the technical field of microbiological assay. The primers for assaying the citrobacterrodentium, provided by the invention, include an outer primer pair, an inner primer pair, and a loop primer pair; sequences of the outer primer pair are shown as SEQ ID NO.1 and SEQ ID NO.2; sequences of the inner primer pair are shown as SEQ ID NO.3 and SEQ ID NO.4; and sequences of the loop primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6. The specific primers provided by the invention havegood specificity and high sensitivity, and can rapidly assay the citrobacter rodentium.

The invention discloses citrobacter, application of the citrobacter in treatment of sulphate-containing waste water, and a separation and identification method of the citrobacter. The citrobacter hasa sulphate reduction capability; the number of the citrobacter is Citrobacter sp.EPS8; the citrobacter is preserved in the Guangdong microbial strain preservation centre on July 13th, 2020; the preservation address is Floor 5, Building 59, Courtyard 100, Xianlie Middle Road, Guangzhou City, Guangdong Province; and the preservation number is GDMCC NO:61090. The strain can take lactic acid as a carbon source, and can reduce sulphate (the reduction rate is 100%) under an anaerobic condition; the strain has relatively strong sulphate reducing capacity within the initial pH value of 6-8; and the optimal initial pH value of the strain is 6.0. The citrobacter disclosed by the invention is facultative anaerobic bacteria, is rapid in aerobic growth, easy to culture and capable of anaerobically reducing sulphate, and has wide application in the aspect of treating wastewater containing SO4<2> (such as acid mine drainage).

The invention relates to a citric acid bacterium and its application in controlling scarabs. The Citrobacter is preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.22672.

The invention relates to the field of microbes, in particular to a sulfate reducing Citrobacter sp.strain HCSR and use thereof. The collection number of the sulfate reducing Citrobacter sp.strain HCSR is CGMCC NO.4660. The sulfate reducing Citrobacter sp.strain HCSR screened by the invention is amphimicrobe; and compared with the conventional sulfate reducing bacteria (SRB) strict anaerobic culture condition, the strain grows quickly under an aerobic condition and has a sulfate reducing characteristic under an anaerobic condition, the SO4<2-> removing capacity is improved to 6g / L from 2.85g / Lwithin 7 days, the facultatively anaerobic physiological property of the strain is fully played, the strain is easy to culture, the difficulty in treatment process is lowered, and the strain can be widely used in the control of sulfate-containing waste water.

The invention discloses an application of Citrobacter sp. JPG1 in removal of bromcresol purple and acid golden yellow G, and belongs to the technical field of microorganisms. The Citrobacter JPG1 is preserved in China General Microbiological Culture Collection Center on December 22, 2016 with the preservation number of CGMCC NO.13480, and the strain has good ability to remove the bromcresol purpleunder an aerobic condition and the acid golden yellow G under an anaerobic condition. When the respectively concentration of the bromcresol purple and the acid golden yellow G is 200 mg / L and the initial inoculum size of the strain is 10%, the removal rate of the p-bromocresol purple at 36 h reaches 73.64%, and the removal rate of the acid golden yellow G at 36 h reaches 87.51%. The Citrobactersp. JPG1 is an efficient dye removal bacterium, can be directly applied to biological treatment of dye wastewater containing bromcresol purple and acid golden yellow G, and has good application prospects in anaerobic and aerobic treatments.

The invention relates to a nucleotide specific to citrobacter O6 and O9 as well as an application of the nucleotide. The nucleotide is at least one of nucleotides shown in SEQ ID NO:1-4. Those nucleotides can be applied to preparation of a PCR (polymerase chain reaction) kit for detecting citrobacter. The citrobacter can be sampled from digestive attracts, urine, blood and crude extracts of wound cultures and can be separated from human and animal waste environment. According to the nucleotide specific to citrobacter O6 and O9 serotypes as well as the PCR kit comprising the nucleotide, the PCR kit is convenient to prepare, short in detection period, high in speed, high in operability, prone to industrial production, lower in detection cost and high in accuracy and sensitivity.

The invention discloses a strain of citrobacter sp. JPG1, which has the capability of secreting riboflavin and also has the capability of removing heavy metal copper and cadmium ions in wastewater. The strain JPG1 is preserved in the China General Microbiological Culture Collection Center in December 2016, and the preservation number of the strain is CGMCC No.13480. The citrobacter JPG1 not only has related genes secreting riboflavin, but also has heavy metal copper and cadmium resistance, can remove heavy metal copper ions and cadmium ions in wastewater at the same time, and has great application potential in the aspect of removing heavy metal in wastewater.

The invention belongs to the field of quality control on the aspect of microbial detection and particularly relates to a sample for verifying the capability of bile salt resistant gram-negative bacteria in a drug and a preparation method thereof. The sample for verifying the capability of bile salt resistant gram-negative bacteria in the drug includes target florae and background florae, wherein the target florae include escherichia coli, klebsiella pneumoniae, enterobacter cloacae, proteus mirabilis, enterobacter aerogenes and citrobacter freundii, and the background florae include staphylococcus aureus and bacillus cereus. The homogeneity and stability meet the capability verifying requirements, the stability of the sample can be ensured to the most degree, and even if the convent of the escherichia coli in transportation and storage changes, and the total amount of the bile salt resistant gram-negative bacteria is also not affected on the aspect of statistical significance. The preparation method of the sample is simple in process and high in success rate.

The invention relates to citrobacter sp. and an application thereof to prevention and treatment of scarabaeidae. The citrobacter sp. is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22672.

The invention belongs to the field of environmental microorganisms, and relates to a Citrobacter strain and an application thereof. The name of the Citrobacter sp. is deodorizing bacteria ZT‑S5, and it was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on December 25, 2017. The strain preservation number is CGMCC No.15120. The Citrobacter strain provided by the invention has high activity and strong adaptability, and can be widely used in the biological treatment process of hydrogen sulfide and ammonia in water bodies and gases. The biological deodorization device made by absorbing the strain can well resist the impact of waste gas and realize the application of biological deodorization.

The invention discloses a method for determining an administration scheme of doxycycline to citrobacter freundii of procambarus clarkii in different administration modes, and relates to the field of drug effects of aquatic drugs. Comprising the following steps: step 1, separating citrobacter freundii from clinically diseased procambarus clarkia, and measuring the MIC value of doxycycline to the citrobacter freundii by adopting a microdilution method; 2, performing single oral administration on the procambarus clarkii by adopting oral administration, intramuscular injection and venous sinus injection methods respectively, and collecting tissue samples respectively after administration; 3, respectively measuring the residual quantity of doxycycline in the tissue samples collected in the step 2; 4, calculating pharmacokinetic parameters including area under a drug time curve, absorption half-life period, elimination half-life period, peak reaching time, peak concentration, apparent distribution volume and clearance rate; and 5, determining a doxycycline administration scheme according to a calculation formula 1. According to the determination method, the medication efficiency and medication safety can be improved.

The present invention relates to the technical field of microbial cultivation, water body purification, and nanomaterial synthesis, and specifically relates to a method of cultivating Citrobacter freundii to remove heavy metal cadmium in water and produce nanomaterial precipitation in situ, especially to a method of Citrobacter freundii decomposes the nutrients around the culture solution to generate a large amount of acid ions, some acid ions such as carbonate and phosphate react with heavy metal cadmium to form cadmium nano-scale precipitation, so as to achieve the removal of heavy metal cadmium in water At the same time, the corresponding nanometer metal materials are also synthesized.

A strain of Citrobacter ZYD‑1 and its application in the degradation of hexabromocyclododecane, the strain is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.16788. The strain is a Gram-negative, white, off-white opaque round colony with a raised surface. Under the scanning electron microscope, it is rod-shaped, with a diameter of 0.5-0.7μm×1-2μm. The facultative anaerobic degrading bacterium ZYD-1 screened in the present invention can use HBCD as the sole carbon source, and has a relatively high degradation rate for HBCD. It was also found that during the anaerobic degradation process, there is a phenomenon of conversion of γ-HBCD to α-HBCD. The invention can be used for the restoration of HBCD polluted sites, especially for anaerobic environments such as sediments and bottom mud.