9 results about "Citrobacter sp" patented technology

The invention relates to the technical field of microorganisms, in particular to a citrobacter welchii bacteriophage as well as a composition and application thereof. The name of the citrobacter werkmanii bacteriophage is RDP-CI25012, the citrobacter werkmanii bacteriophage is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on August 25, 2025, and the preservation number of the citrobacter werkmanii bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No. 46668. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing citrobacter welchii; (2) preparing a product for inhibiting citrobacter welchii; and (3) application in preparation of a product for preventing and / or treating citrobacter welchii disease caused by citrobacter welchii. The cracking rate of the phage is 80%, and the phage has an extremely high host range and a relatively wide cracking spectrum. After the bacteriophage is treated at 50 DEG C for 4 hours, the titer is still kept at 107 PFU / mL or above, and the bacteriophage has excellent thermal stability, so that a solid foundation is laid for use, storage, transportation and production processing of the bacteriophage in most practical application scenes.

The invention provides a citrobacter freundii phage and an application thereof. The bacteriophage is a citrobacter freundii bacteriophage CFP2505001, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M20251306. The bacteriophage has excellent lysis activity, thermal stability and pH stability, can efficiently perform targeted lysis on multi-drug-resistant citrobacter freundii, significantly reduces the infection death rate of Japanese eels, and provides a new microbial resource and application approach for green prevention and control of citrobacter freundii diseases in aquaculture.

The invention discloses citrobacter freundii, a product and application, and belongs to the technical field of microorganisms. The preservation number of the citrobacter freundii is CGMCC (China General Microbiological Culture Collection Center) No.36707, and the preservation number of the citrobacter freundii is CGMCC The strain not only meets the basic biological safety requirement, but also can efficiently and specifically activate a key cGAS-STING innate immune signal channel in a host cell by virtue of a culture supernatant, so that the expression levels of the type I interferon and a downstream effect gene thereof are remarkably up-regulated. Due to the unique immunoregulation function, the compound shows a good inhibition effect on replication of herpes simplex virus and vesicular stomatitis virus, and a new resource and a feasible path are provided for developing an antiviral biological preparation which is based on flora immunoregulation and has broad-spectrum potential.

The present application relates to the field of microbial technology, in particular to a Citrobacter werkmanii bacteriophage and its composition and application. The Citrobacter werkmanii bacteriophage (Citrobacter werkmanii bacteriophage) is named RDP-CI25012, which was preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number is CGMCC No. 46668. A composition comprises the above-mentioned bacteriophage. The above-mentioned bacteriophage or composition is used in (1) preparing a product for killing Citrobacter werkmanii; (2) preparing a product for inhibiting Citrobacter werkmanii; (3) preparing a product for preventing and / or treating Citrobacter werkmanii disease caused by Citrobacter werkmanii. The phage has a lysis rate of 80%, has a very high host range, and has a wide lysis spectrum. The phage has excellent thermal stability, and the titer remains above 10 7 PFU / mL after being treated at 50 DEG C for 4 hours, which lays a solid foundation for its use, storage, transportation and production processing in most actual application scenarios.

The application provides a composition of multiplex PCR primers and probes for detecting microorganisms, a kit and a method thereof. The composition comprises 10 pairs of primers for staphylococcus aureus, salmonella, pseudomonas aeruginosa, clostridium tetani, citrobacter freundii, streptobacterium maltophilia, burkholderia cepacia, klebsiella pneumoniae, candida albicans and escherichia coli, and 10 corresponding probes, wherein the 10 pairs of primers are shown as SEQ ID No. 1-20, and the 10 probes are shown as SEQ ID No. 21-30. The composition of primers and probes of the application is used for multiplex PCR for detecting microorganisms, is simple to operate, and has high detection accuracy.

The invention relates to a suitable citrobacter strain and application thereof. The invention relates to citrobacter suitabilis strain J35, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the citrobacter suitabilis strain J35 is CGMCC No.36708, and the citrobacter suitabilis strain J35 has insecticidal activity on Sitobion avenae, Rhopalosiphum padi, Amyzus persicae, Lygocoris lucorum, Bemisia tabaci, Plutella xylostella and Spodoptera litura. The citrobacter suitabilis strain J35 has the advantages that the strain J35 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the citrobacter suitabilis strain J35 is CGMCC

The invention discloses citrobacter freundii and application thereof, the citrobacter freundii is excellent in performance and capable of producing hyaluronidase at high yield, and the obtained hyaluronidase is high in enzyme activity and excellent in performance and has wide application prospects.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.

To provide Citrobacter portucalensis, a hyaluronidase as well as preparation methods and applications of the hyaluronidase.SOLUTION: Disclosed herein is Citrobacter portucalensis strain HA2301, deposited in the China Center for Type Culture Collection on November 01, 2023 with the deposition number of CCTCC NO.M20232108. The invention further discloses a hyaluronidase and a preparation method and application thereof. According to the Citrobacter portucalensis, the hyaluronidase, preparation method and application thereof described in the invention, the Citrobacter portucalensis produces the hyaluronidase that enzymatically degrades high molecular weight sodium hyaluronate to prepare small molecular weight and oligomeric sodium hyaluronate.SELECTED DRAWING: Figure 1