Natural deficient mutant strain of PhoP gene existing in clinical isolates of mycobacterium tuberculosis in Xinjiang areas

Abstract

The invention relates to a natural mutant strain of mycobacterium tuberculosis PhoP of clinical isolates of mycobacterium tuberculosis in sputum samples of patients with tuberculosis in Xinjiang areasin the field of study on microbes. By collecting sputum samples of patients with tuberculosis in the department of infectious diseases in multiple Xinjiang areas, 438 clinical isolates are obtained,and by total DNA extraction, ordinary PCR (polymerase chain reaction) and fluorescence quantitative PCR, the natural mutant strain of PhoP in the clinical isolates is discovered.

Description

technical field [0001] The present invention relates to the gene mutation of mycobacterium tuberculosis in the field of microorganism research. Background technique [0002] With the increasingly convenient transportation, increasing population mobility, and the widespread application of clinical immunosuppressants, the current tuberculosis epidemic has once again sounded the alarm. The main pathogenic bacteria of tuberculosis is Mycobacterium tuberculosis, and the research on the mechanism of Mycobacterium tuberculosis is not completely clear yet. [0003] PohP belongs to a two-component signal transduction system, which is generally believed to be ubiquitous in prokaryotes. It consists of a histidine kinase and a response regulator. Some literatures believe that it is related to the adaptability of bacteria to the host and the virulence of bacteria. The function of the standard strain of Mycobacterium tuberculosis (H37Rv) has been extensively studied. [0004] Xinjiang i...

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Problems solved by technology

It is still impossible to obtain band samples by ordinary PCR. We use real-time fluorescent quantitative PCR to detect the molecular level and find that the presence of PhoP cannot be detected at the molecular level.

[0006] We believe that during the transmission of Mycobacterium tuberculosis, whether it is due to environmental factors or host factors, the bacteria found a mutation, whether this mutation is beneficial or harmful to itself or the flora, and the strain still survived after the deletion, and the bacteria Are there other alternative mechanisms that have not been reported so far

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