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Method for dissecting pupa brain of lepidopteran insect dead by freezing but with undamaged brain

A lepidopteran, cool technology, applied in the field of insect anatomy, can solve problems such as difficult dissection, and achieve the effect of avoiding structural damage

Inactive Publication Date: 2019-05-31
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The brain in the pupa is in the state of developing from a larval brain (underdeveloped, primitive) to an adult brain. The immature epithelial membrane dissociates and causes the brain to be deformed, and the internal tissue structure of the brain is loose and flocculent. It is difficult to dissect a complete brain for follow-up research

Method used

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  • Method for dissecting pupa brain of lepidopteran insect dead by freezing but with undamaged brain
  • Method for dissecting pupa brain of lepidopteran insect dead by freezing but with undamaged brain

Examples

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Embodiment 1

[0021] A method for dissecting the pupal brain of Lepidoptera insects that have been frozen to death and have no brain damage, comprising the following steps: after cutting off the pupae's head shell, washing the blood plasma in the head shell with cold physiological fluid, exposing the brain and fixing it with fixative solution The brain inside the pupal head shell is pinched off the membranous matter attached to the head shell, and then the formed brain is cut out to obtain a complete brain tissue.

[0022] The specific steps are:

[0023] (1) Quickly cut off the head shells of lepidopteran pupae that were frozen to death and had no brain damage;

[0024] (2) Use a straw to suck up the cold physiological fluid, wash away the unformed plasma fluid in the head shell of the pupa, and expose the brain;

[0025] (3) Drop the fixative solution to fix the brain for 10-30 minutes. After the brain is fixed, it will no longer be limp; the time for fixing the brain of the present inve...

Embodiment 2

[0033] A method for dissecting the brain of cotton bollworm pupae that is frozen to death and has no brain damage, the specific steps are as follows:

[0034] (1) Place the collected pupae of cotton bollworm on ice, and cut off the head shell;

[0035] (2) Use a plastic straw to suck up the cold physiological fluid, wash away the chaotic unformed plasma fluid in the skull, expose the brain, and then put it on the bottom of the petri dish;

[0036] (3) Use a plastic straw to absorb a small amount of fixative solution into the skull, fix it for 10 minutes, and the brain is no longer paralyzed.

[0037] (4) Put a drop of Ringer's physiological solution on the bottom of the petri dish, one drop is about 0.1ml, fix it with fine anatomical tweezers with the left hand, and gently pinch off the membrane attached to the head shell with the tweezers in the right hand, and then use the fine tweezers to remove the head shell The trachea and other tissues around the brain are removed to o...

Embodiment 3

[0039] A method for dissecting brains of P. tabacum pupae that are frozen to death and have no brain damage. The specific steps are as follows:

[0040] (1) Place the collected pupae of the tobacco budworm on ice, and cut off the head shell;

[0041] (2) Use a plastic straw to suck up the cold physiological fluid, wash away the chaotic unformed plasma fluid in the skull, expose the brain, and then put it on the bottom of the petri dish;

[0042] (3) Use a plastic straw to absorb a little fixation liquid and drop it into the skull, fix it for 20 minutes, and the brain is no longer paralyzed;

[0043] (4) Drop a drop of Ringer's physiological solution on the bottom of the petri dish, fix it with fine dissecting forceps in the left hand, and gently pinch off the membrane attached to the skull with the forceps in the right hand, and then use fine forceps to remove the trachea and other tissues around the brain Removed to obtain complete brain tissue.

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Abstract

The invention discloses a method for dissecting the pupa brain of a lepidopteran insect dead by freezing but with undamaged brain. The method comprises the following steps: after the skull of a pupa is cut off, the plasma in the skull is washed by cold physiological liquid, the brain in the skull of the pupa is fixed by fixing liquid after the brain is exposed, the membrane attached to the skull is clamped off, and then the formed brain is dissected out to obtain complete brain tissue. Compared with the prior art, the whole lepidopteran insect pupa brain can be obtained, the original state ofthe ultrastructure in the brain is completely maintained, structural damage is avoided, and the method is beneficial to obtaining of good results in a next experiment.

Description

technical field [0001] The invention belongs to the field of insect anatomy, and in particular relates to a method for dissecting the brains of lepidopteran insect pupae which are killed by freezing and whose brains are not damaged. Background technique [0002] Lepidoptera insects, including butterflies and moths, such as cotton bollworm and cabbage butterfly, are important pests in agriculture and forestry, and often cause catastrophic losses to agriculture and forestry. [0003] At present, the control of Lepidoptera pests mainly adopts various methods such as chemical control, physical control, and natural enemy control, but each method has its limitations. The central nervous system of insects plays an important role in regulating insect behavior and regulating growth and development. The research on the structure and function of the insect brain has become a research hotspot in the field of insects, especially the research on the sense of smell and taste of insects is...

Claims

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Application Information

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IPC IPC(8): A61D1/00
Inventor 赵新成陈文波谢桂英常亚军鲁凯
Owner HENAN AGRICULTURAL UNIVERSITY
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