12results about How to "Keep it pristine" patented technology

The invention discloses a plant cell nucleus extraction method and application, and belongs to the technical field of plant single cell sequencing. The method comprises the following steps: carrying out tissue immobilization on plant tissues by using precooled methanol; preparing a cell nucleus lysis solution, namely an NIB solution; cleaning and chopping the fixed tissue sample, and incubating the tissue sample in an NIB solution; filtering with a screen; centrifuging, discarding supernatant, and resuspending and precipitating the NIB solution to obtain a suspension; slowly dripping the suspension into a Percoll solution, and carrying out density gradient centrifugation to purify cell nucleuses. The invention provides a high-applicability and high-quality cell nucleus extraction method which is suitable for cryopreserved or fresh tissue samples, higher cell nucleus yield and mRNA capture rate are obtained, and the data quality of single cell sequencing is improved.

The utility model discloses a leather cutting machine partition adsorption structure which comprises a rack, a cutting panel is arranged at the top of the rack, a plurality of adsorption holes are formed in the cutting panel, an air pipe is arranged below the cutting panel, the air pipe is externally connected with a vacuum pump, and the air pipe corresponding to the adsorption holes is connected with the bottom face of the cutting panel through a branch air pipe. An air cylinder is arranged corresponding to each branch air pipe, the output end of each air cylinder is connected with a partition plate, and the partition plates can stretch into the ends of the branch air pipes to open or close the branch air pipes. The vacuum adsorption mode is adopted for fixing the dermis, damage to the dermis is small, obvious traces or tensile deformation cannot be left on the surface of the dermis, the original state and quality of the dermis can be well kept, the vacuum adsorption type dermis fixing device can adapt to dermis of various shapes and sizes, and regular rectangles or irregular shapes can be effectively fixed.

The application provides a point cloud completion method and device and a storage medium, which comprises the following steps: collecting original images and stripe images of an object through a binocular structured light system; reconstructing a point cloud with high precision but with reflection and dark area missing by using the stripe images; meanwhile, calculating a disparity map by using a FoundationStereo deep learning model for stereo matching based on the original images to obtain a point cloud which is complete but has lower precision than the stripe image reconstruction; in order to meet the point cloud coordinates in the two three-dimensional reconstruction methods, the point cloud coordinates of the two reconstruction methods are corrected based on a standard ball multi-position reconstruction data calculation error model; firstly, the two point clouds are spatially registered and aligned; then, the missing area of the high-precision point cloud is analyzed, the corresponding area in the low-precision point cloud is located through image mapping, and the point cloud of the corresponding area is extracted as a patch. The application effectively solves the data missing problem of the structured light measurement in the reflection and dark area, and realizes the balance between the point cloud precision and completeness.

The utility model relates to a woven bag capable of placing articles in multiple layers, which comprises a bag body and a handle, the handle is fixedly mounted above the bag body, an accommodating cavity is arranged in the bag body, the woven bag further comprises an interlayer positioned in the accommodating cavity, the interlayer comprises a positioning plate at the top, a positioning groove for placing the positioning plate is arranged on the bag body, and the positioning plate is arranged in the positioning groove. The positioning plate is located in the positioning groove and detachably connected with the bag body. By the adoption of the scheme, after the interlayer is placed in the bag body, the containing cavity is divided into two parts, different kinds of articles are placed at the bottom of the bag body and in the interlayer, then the articles are placed in different areas, and the articles can be taken out according to requirements.

The invention discloses a rapid detection device and method for three-dimensional space and time distribution of defects in materials and devices, and belongs to the field of material detection. The core function modules specifically comprise a laser module, a light beam splitting module, a frequency conversion module, a super-continuum spectrum generation module, an optical delay line module, a laser scanning confocal microscope module, a spectrum detection module and a data processing unit. Four-dimensional detection of space three dimensions and time one dimensions is realized, and a quantitative structure-activity relationship between defects and device performance is established for four-dimensional comprehensive representation of space snapshots and time snapshots of materials. The confocal technology and the super-resolution technology are combined, the nanoscale spatial resolution and the femtosecond time resolution are considered, and the large-area, multi-dimensional, high-precision and rapid detection requirements are met.

The present invention discloses a crushing and isolating device for recycling waste lithium batteries, which relates to the technical field of general crushing, and includes a frame, the frame is fixedly connected with an oxygen content monitor, the frame is rotatably connected with wolf-tooth crushing rollers symmetrically distributed along the frame, the frame is fixedly connected with an air pump, the oxygen content monitor is electrically connected to the air pump through a control module, an air pipe is connected between the air pump and the frame, the frame is fixedly connected with a support frame for stabilizing the air pipe, the frame is provided with an exhaust valve for limiting the one-way discharge of gas, the upper part of the frame is connected with a feed frame, the air pipe is connected with the feed frame, and the upper part of the feed frame is horizontally slidably connected with a cover plate. The present invention adopts a wolf-tooth crushing roller, which mainly relies on low-speed operation to tear and shear and break up to complete the material crushing operation, can effectively crush and break up the material, does not need to add other crushing and breaking up equipment, and has low cost.

The invention relates to the technical field of shock crater quantification, discloses a shock crater high-precision quantitative analysis method based on three-dimensional laser scanning, and solves the technical problem of relatively large error in tangent plane two-dimensional measurement in the prior art. The analysis method comprises the following steps: step 100, performing mold reversing on an inner concave structure formed by a to-be-tested impact crater to form a to-be-tested crater convex mold with an outer convex structure matched with the inner concave structure; step 200, scanning and processing the to-be-tested crater male die by using a three-dimensional laser scanner to obtain three-dimensional grid data of the to-be-tested impact crater, and establishing a cylindrical coordinate system; step 300, analyzing the three-dimensional grid data in a cylindrical coordinate system, obtaining a crater section curve of the to-be-measured impact crater, and obtaining the crater depth and the crater diameter of the to-be-measured impact crater based on the crater section curve; and Step 400, inputting the three-dimensional grid data into three-dimensional visualization software, and obtaining the crater volume of the impact crater to be measured.

The utility model provides a geological exploration sample storage device, including box body, inner pad, the bottom fixedly connected with inner pad in the inside of box body, the inside fixedly connected with the baffle of box body. The utility model has the advantages of: single sample is placed in single lattice, and the soft gel is compacted, and the sealing membrane can be closely combined with the geological sample, effectively prevent the sample from being damaged by the external physical impact in the transportation or processing process. The soft gel provides additional buffering effect, further reduces the risk of sample damage due to vibration or bumping, the sealing membrane and the soft gel can isolate the sample from the external environment, prevent moisture, dust, chemical substances and other pollutants from entering the sample, thereby maintaining the original state of the sample and ensuring the accuracy of subsequent analysis. The use of sealing membrane and soft gel simplifies the sample processing process. Compared with the traditional wrapping method, they are easier to operate, and no additional fixing or supporting equipment is needed, thereby improving the work efficiency.

The invention relates to a method for detecting and / or screening a target protein expression cell line based on fluorescence indication, and aims to realize rapid detection of specific protein conformation in chassis cells and efficiently screen chassis cells with different functional states, activities and target protein expression levels. The essential difference between the technology and a conventional protein fusion fluorescent label for indicating protein is that the conformation change of the protein is reflected through a cross-linking agent, and the functional state corresponding to the conformation of the protein is reflected based on the change of the fluorescence color of a cross-linking agent skeleton under different protein conformations. The technology provides a convenient, efficient and non-invasive method for the field of biological research, and can be widely applied to the fields of cell activity research, drug screening, protein function research and the like.

According to the ceramic capacitor grain size measurement method based on ion beam sample preparation and imaging, clear grain morphology is obtained by carrying out mechanical grinding and polishing sample preparation and ion beam polishing on a ceramic capacitor sample, so that accurate measurement of the grain size is realized, and the method has important significance in improving the performance and reliability of the ceramic capacitor. The method can be popularized and applied to other fields needing grain size measurement, and has a wide industrial application prospect.

A dynamic liquid integrated single cell surface enhanced Raman scattering (SERS) detection platform comprises a distortion mixing part, a 3D flow focusing part and a single cell SERS detection part which are connected in sequence, the twisting and mixing part is used for mixing inflowing cells and a multifunctional modified nano probe through a spirally twisted mixing micro-fluidic chip, and a mixed cell solution combined with the multifunctional modified nano probe is delivered to the 3D flow focusing part and is subjected to 3D focusing in the center of the 3D flow focusing part to form a single row; finally, flowing through a laser area of the single-cell SERS detection part, and collecting an SERS spectrum for detection; through the arrangement of the micro-fluidic chip and the coaxial needle, a cell solution and a multifunctional modified nano-probe solution are guided into a twisted mixing channel to be efficiently mixed and then focused, the sample treatment process is simplified, and the Raman detection reaches the single cell level in cooperation with the use of the multifunctional modified nano-probe; the method belongs to label-free detection, the requirement for pretreatment of the cells is reduced, the original state of the cells is kept, and downstream detection can be carried out; the kit has the advantages of high sensitivity, low cost, simplicity and convenience in operation, convenience in data analysis, cell viability maintenance and the like.