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51results about How to "Keep it pristine" patented technology

Bean curd and bean curd production method

The invention discloses bean curd and a bean curd production method. The bean curd production method comprises the following steps: preparing soybean milk; and mixing the soybean milk with a coagulant, coagulating to obtain bean curd. The preparation of the soybean milk comprises 1) adopting soybean cotyledon particles to serve as a raw material after breaking, embryo removal and soybean skin removal; 2) grinding after soaking the soybean cotyledon particles in water for 30-60 minutes, removing bean dregs after grinding so as to obtain raw soybean milk, and enabling the weight ratio between the cotyledon particles and water for soaking the cotyledon particles to be 1:6-11 during soaking; and 3) boiling the raw soybean milk, maintaining the temperature at over 85 DEG C for 30-45 minutes toobtain the soybean milk. The bean curd production method is scientific and simple, overcomes various shortcomings in the prior art, enables coagulating process of the soybean milk to be effectively adjusted, is favorable for achieving industrialized production of bean curd, and has the advantages of saving energy, simplifying equipment and reducing sewage discharge. Besides, the quality of the produced bean curd is equal to or close to that of northern bean curd.
Owner:FOODSTUFF INST BEIJING

Device for sampling column shape samples of shallow water deposit

The invention discloses a sampling device for a column-shaped sample of shallow water sediment which includes a sampling tube component, a stress application component and a connector component arranged between the sampling tube component and the stress application component; the sampling tube component includes a sampling tube; the connector component includes a lower flange and a connecting piece connected by a bolt, a sealing piece arranged between the lower flange and the connecting piece, a connector connected on the connecting piece and a holding valve arranged in the connector and the connecting piece; the stress application component includes a plurality of hollow connecting rods connected on the connector and the top parts of the connecting rods are provided with ventilating screwed plug; the sampling tube passes through the lower flange and a lock ring, extents into the connecting piece and is fixedly connected on the lower part of the connecting component through a bolt. By adopting the invention, the initial state of the column-shaped sample of the shallow water sediment can be maintained and good sampling effect is obtained. The invention has the advantages of compact structure, easy carry, convenient operation and being able to adapt various field sampling conditions.
Owner:CHINA INST OF WATER RESOURCES & HYDROPOWER RES

Classification device of metal powder and using method

The invention discloses a classification device of metal powder and a using method, and belongs to the technical field of metal powder for 3D printing. The classification device comprises a powder storage tank, a support, a wind motor, a classification wheel, a primary powder collection tank, an induced draft fan and the like. The powder storage tank is inversely placed on the support. A classification motor is located above a barrel and directly connected with the classification wheel. The using method comprises the steps that powder is loaded into the powder storage tank, a ball valve is closed, the powder storage tank is inversely placed on the support, a classifier frequency converter, an induced draft fan ventilation door and a secondary air valve are started after it is determined that connection is errorless, the power of the induced draft fan is 2-5 KW, the power of the classification wheel is adjusted to be 30-50 KW, a pulse instrument carries out cyclic injection for 4-10 min after classification and then is turned off, and sampling is carried out for grain size analysis. The classification device has the advantages that pollution is effectively reduced, the working intensity is lowered, and product quality is remarkably improved.
Owner:中航迈特增材科技(北京)有限公司 +1

Preservation reagent for microorganism specimens containing rich organic matters, kit containing preservation reagent and application of preservation reagent

The invention discloses a preservation reagent for microorganism specimens containing rich organic matters, a kit containing the preservation reagent and an application of the preservation reagent. The preservation reagent comprises a buffer solution, a microorganism cell protective agent, a chelating agent and an ion strength maintenance agent. The preservation reagent contains simple and easy-to-prepare ingredients, is simple and convenient to prepare and low in cost, has a good preservation effect on microorganisms in the specimens with rich organic matters, can guarantee the diversity and the corresponding proportion of the microorganisms in the specimens at the normal temperature or even at the higher temperature, maintains the microorganism unchanged in at least 14 days, is applicable to outdoor sampling and the conditions that immediate frozen storage is not convenient after sampling or frozen storage is unavailable, has lower requirements for the transportation conditions and facilitates storage and transportation of the specimens. With the use of the preservation reagent, storage of the specimens containing the rich organic matters is facilitated, no influence is produced on following extraction of total DNA, PCR amplification, high-throughput sequencing and the like of microorganisms, and the experiment efficiency can be further improved.
Owner:TINYGENE BIOTECH SHANGHAI CO LTD

Multi-strain co-immobilized fermented papaya vinegar and preparation method thereof

The invention discloses novel multi-strain co-immobilized fermented papaya vinegar and a preparation method thereof. The preparation method comprises the steps of papayas selection, cleaning, blanching, peeling, seed removal, cutting, pulping, enzymolysis, centrifugation, component adjustment, pasteurization, cooling, co-immobilized alcohol fermentation, immobilized acetic bacteria fermentation, clarification, filtration, sterilization, ultrafiltration membrane purification, filling and product finishing. According to the novel multi-strain co-immobilized fermented papaya vinegar and the preparation method thereof, the problems that multiple papayas in the mature season are difficult to store, the traditional fruit vinegar fermentation is complicated to operate and the strains are liable to be deactivated can be solved effectively; the papayas can be used for batched fruit vinegar production, the production cycle is reduced and the production capacity is improved; the prepared papaya vinegar is clear and transparent, has stable color, is orange-yellow, has the prominent fragrance of the papayas, is faint scent, has mellow taste and soft mouthfeel, is good for a human body to digest and absorb food, and has the characteristics of invigorating spleen to promote digestion, prolonging life and the like.
Owner:AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI

Blockchain data sharing method, system and device and medium

The invention discloses a blockchain data sharing method, a system and a device and a medium. The method comprises the following steps: creating member institutions in a blockchain; according to the member institutions, carrying out initialization processing on blockchain nodes, and creating a data chain, a rule chain and an application chain in an alliance chain; standardizing a data source of the data institution, and auditing and registering a standardized processing result through the supervision institution; issuing metainformation and authority rules of a target data source to the blockchain through a first data institution, and adopting the supervision institution to confirm the target data source and then issues the target data source to the data chain; adopting a secret key encryption method to initiate a data request to the target data source by a second data institution, and obtaining the target data source; and determining evaluation information of the target data source bythe second data institution, and storing the evaluation information in the application chain. Data privacy can be protected, data sharing efficiency can be improved, and the method can be widely applied to the technical field of block chains.
Owner:SUN YAT SEN UNIV

In situ determination method of enzymatic activity in soil

The invention relates to the technical field of in situ zymogram, in particular to an in situ determination method of enzymatic activity in soil. The determination method comprises the following stepsof planting plants in an inclined root box until root systems of plants grow out; preparing an enzymatic activity fluorescence labeling substrate solution; soaking a microporous filtering film into the enzymatic activity fluorescence labeling substrate solution; covering the microporous filtering film onto the surfaces of the root systems; covering a light shielding film above the microporous filtering film; after the first side surface is buckled, downwards putting the first side surface of the root box into a culture box to be cultured; taking out the microporous filtering film; putting themicroporous filtering film under an ultraviolet lamp tube; taking photos by a digital camera in a position right above the film; calculating the enzymatic activity of the corresponding region of themicroporous filtering film by using picture grayscale analysis software. The determination method has the advantages that the accuracy is high; the space and time resolution ratios are high.
Owner:NORTHWEST A & F UNIV

Dynamic balancing measurement machine

The invention relates to a dynamic balance measuring machine, which comprises a stand. A gear box is arranged at the stand, an input shaft and an output shaft are arranged in the gear box, the output shaft is connected with a main shaft of an electric jigger through a clutch, and a main shaft of the electric jigger is driven by an electric motor to rotate forwards and reversely; and a speed change mechanism is arranged between the input shaft and the output shaft in the gear box, and the speed change mechanism comprises a speed change gear and a gear selecting device which are engaged with each other. The invention mainly provides the dynamic balance measuring machine in which the gear box has wide working range and comparatively high raising speed, can bear positive and negative torque, carries out bidirectional measurement and control, and is applied to large-vibration environment; and the dynamic balance measuring machine solves the technical problems that the dynamic balance measuring machine has limited application range, can only bear unidirectional torque and carries out unidirectional measurement and control in the prior art.
Owner:杭州南方通达齿轮有限公司

Method for preparing enzyme by using honeysuckle and curcuma longa as main raw materials

The invention relates to a method for preparing an enzyme by using honeysuckle and curcuma longa as main raw materials. The method specifically comprises: (1) selecting 35-45 parts by weight of honeysuckle, 10-15 parts by weight of musa basjoo siebold, 10-15 parts by weight of pineapple, and 10-15 parts by weight of clausena lansium, and washing with clear water so as to be spare; (2) peeling the clausena lansium to expose the flesh, cutting the pineapple with the peel and the musa basjoo siebold with the peel into pineapple slices with the thickness of 1-2 cm and musa basjoo siebold slices with the thickness of 1-2 cm, and uniformly mixing the slices to obtain a mixture; (3) placing the mixture into a sealed container, adding honey and water, uniformly mixing, placing into a room, carrying out sealed fermentation at a room temperature, and maintain for 1 month, wherein stirring is performed everyday at the starting 5-7 days and stirring is performed every 7 days at the later days; and (4) washing fresh curcuma longa picked before and after winter solstice, cutting into curcuma longa slices with the thickness of 0.3-2 cm, taking 15-25 parts by weight of the curcuma longa slices, adding to the sealed container, and carrying out sealed fermentation for more than 3 months so as to obtain the product. According to the present invention, the enzyme prepared through the method has advantages of original food function maintaining, rich probiotics, rich nutrition, good taste, and long shelf life.
Owner:唐妙红

Freely combined low enriched uranium foil target with multiple single pieces

The invention belongs to the technical field of radioactive substance preparation, and relates to a freely combined low enriched uranium foil target with multiple single pieces. The freely combined low enriched uranium foil target is used for producing fission <99> Mo by means of irradiation. The freely combined low enriched uranium foil target comprises a target barrel, low enriched uranium foils, a target core plug, a pressure plug and sealing covers. The target core plug is placed in the hollow target barrel, so that the multiple pieces of freely combined low enriched uranium foils can be compressed between the inner wall of the target barrel and the target core plug; the target core plug is compressed by the pressure plug in the target barrel from the upper side of the target core plug; two ends of the target barrel are sealed by the sealing covers by means of welding. The freely combined low enriched uranium foil target with the multiple single pieces has the advantages that processes for preparing the freely combined low enriched uranium foil target can be simplified, the irradiation efficiency can be improved, self-shielding can be reduced, the freely combined low enriched uranium foil target is safe in reactor irradiation periods and is easy to release in follow-up procedures, and the quality of the produced <99> Mo can be guaranteed.
Owner:CHINA INSTITUTE OF ATOMIC ENERGY

Method for preparing enzyme by using lemons and galangal as main materials

The invention discloses a method for preparing enzyme by using lemons and galangal as main materials. The method comprises the specific steps that 1, 35-45 parts of lemons, 15-20 parts of galangal, 2-5 parts of turmeric, 8-15 parts of rhizoma zedoariae, 8-15 parts of grapefruit and 8-15 parts of musa basjoo are selected and cleaned for usage; 2, the lemons are firstly extruded to make peels broken and expose pulp, then the galangal, the turmeric, the rhizoma zedoariae, the grapefruit and the musa basjoo are cut into galangal slices, turmeric slices, rhizoma zedoariae slices, grapefruit slices and musa basjoo slices which are 0.3-2 cm in thickness, and then the materials are mixed evenly to obtain a mixture; 3, the mixture is put in a sealed container, honey and water are added for even mixing, then the mixture is put in a room, sealed fermentation is performed under room temperature condition, one time of stirring is performed every day in previous 5-7 days, later one time of stirring is performed every 7 days, the cycle is kept for one month, then sealing is performed, and fermentation continues for 6 months. The enzyme prepared by adopting the method retains the effects of original foods, is rich in probiotics and nutrition and long in shelf life and has a good taste.
Owner:唐妙红

Method for obtaining highly purified and intact soybean hypocotyls

The present invention relates to a method for processing soybean (or soya) material, which is mainly a mixture of soybean hypocotyls and soybean cracks. In particular, the present invention provides a method for separating a soybean hypocotyl fraction from a soybean crack fraction based on a difference in form, by subjecting said soybean material to a centrifugal force, preferably by use of an indented cylindrical device. On beforehand, it is preferable to remove the main part of the soybean hulls, soybean cotyledons, whole soybeans, and the larger soybean crack fraction from the soybean material by means of traditional separation methods based on differences in size and / or weight (e.g. aspiration). The invention also provides a soybean hypocotyl concentrate and uses thereof, e.g. for the preparation of a soybean hypocotyl (or germ or embryo) oil and / or food products.
Owner:ALPRO COMM VA

Double strain co-immobilized fermented papaya wine and preparation method thereof

The invention discloses a double strain co-immobilized fermented papaya wine and a preparation method thereof. The preparation method comprises the steps of papaya selection, cleaning, peel and seed removing, breaking, pulping, enzymolysis, centrifugation, ingredient regulating, pasteurization, cooling, immobilized saccharomyces cerevisiae and aroma producing yeast fermentation (primary fermentation), clarification, filtration, secondary fermentation, ageing, blending, filtration, ultrafiltration membrane purification, filling and finished product obtaining. According to the double strain co-immobilized fermented papaya wine and the preparation method thereof, the problems that in the papaya mature season, the amount of papayas is large, the papayas are not prone to storage, for traditional fermentation wine, operation is tedious, and strains are prone to inactivation can be effectively solved; the papayas can be used for continuously producing fruit wine on a large scale, the production cycle is shortened, the production capacity is improved, the strains can be reused, and the production cost is reduced; the obtained papaya wine has the advantages that the papaya wine is clear and transparent, the color and luster are stable and orange yellow, the fragrance of papayas is prominent, the papaya wine is rich in fragrance, soft in taste and beneficial for digestion and absorption of foods by human bodies, and the effects of invigorating spleen to promote digestion and prolonging life are achieved.
Owner:AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI

Undisturbed soil sampler

PendingCN109655307AAccurate control of sampling volumePrecise control of disturbancesWithdrawing sample devicesDrill bitDrill pipe
The invention discloses an undisturbed soil sampler, which comprises an inner drilling rod, a rotary handle and an inner drilling bit respectively connected with the upper and lower ends of the innerdrilling rod, an outer drilling bit sleeved outside the inner drilling bit, an outer drilling rod sleeved outside the inner drilling rod and connected with the outer drilling bit, a hammering platformconnected with the top end of the outer drilling rod, a round hammer and a sample storage box, wherein a plurality of plug holes are formed in the inner drilling rod and the outer drilling rod, and the inner drilling rod and the outer drilling rod penetrate through the plug holes through a plug to fix the inner drilling rod and the outer drilling rod; a seal is arranged at the top of the inner drilling bit, an exhaust hole is formed in the side wall, and a plurality of knife edges are arranged at the bottom, the inner drilling bit drills through the knife edges, blocks the soil from enteringthe inner drilling rod through the seal, and exhausts air of the inner drilling bit through the exhaust hole; the round hammer impacts the hammering platform after removing the rotary handle and pulling out the plug, so that the outer drilling bit continues to drill along with the outer drilling rod; a convergent opening is formed in the bottom of the outer drilling bit to collect the soil; and the sample storage box is aligned with the convergent opening to store soil in the outer drilling bit. The device is low in cost, simple and practical, accurate in sampling and easy to carry.
Owner:CHINA UNIV OF GEOSCIENCES (WUHAN)

Bean curd and bean curd production method

The utility model discloses a mixer, which is provided with at least one thin conveying pipe, a thick conveying pipe and a mixed pulp output pipe, wherein one end of the thick conveying pipe is closed, and the mixed pulp output pipe is communicated with the side wall of the thick conveying pipe close to the closed end and enables material flowing direction to be changed. At least one thin conveying pipe penetrates into the thick conveying pipe from closed end, a part of the thin conveying pipe penetrating into the thick conveying pipe is larger than a communicating port of the thick conveying pipe and the mixed pulp output pipe, and the part of the thin conveying pipe and the thick conveying pipe form partial overlap. The opening diameter formed from the communicating port of the thick conveying pipe and the mixed pulp output pipe to a discharging port is increased. The mixer overcomes a plurality of defects of the prior art and achieves efficient and stable mixing of two fluids with large flow-quantity difference.
Owner:FOODSTUFF INST BEIJING

Wheat germ processing method by using microwave curing technology

The present invention relates to a wheat germ processing method by using a microwave curing technology. The method comprises the following steps: wheat germ extracting, first microwave drying, plane rotary screen filtering, magnetic substance removing by a magnetic selector, second microwave drying and cooling, inspecting and packaging. The first microwave drying is used to reduce the water content of the wheat germ from about 15% to within 4.8%, then the second microwave drying is used to reduce the water content within 3%, and all the processing temperatures are controlled within 120 DEG C, which avoids loss of protein nutrients at the high temperatures. In the microwave heating process, a variety of active enzymes and harmful microorganisms in the wheat germ can be fully inactivated. The processing method eliminates the raw and smelly tastes of the wheat germ, avoids the rancidity of the wheat germ, meets a long-term storage requirement, and maintains the original state and taste of the wheat germ, so that the wheat germ is crisp in mouthfeel and good in rehydration, and can be dissolved once the wheat germ is put into water without the needing of crushing again.
Owner:扬州市金谷农副产品专业合作社

Preparation method of fish bait preservative cultivated by bioengineering technology

The present invention discloses a preparation method of a fish bait preservative cultivated by a bioengineering technology. The preparation method comprises a mother seed selecting and cooling step, adistilling and material preparing step of fermented grains, an inoculating step, a detecting step and a packaging step. The manufacture principles of the fish bait preservative manufactured by usingthe method are as follows: high-quality distiller's yeasts are optimized to obtain associate strains of yeasts, lactic acid bacteria and enzymes thereof; high-quality starch is used as a main material; plant proteins and plant fibers are auxiliary materials; the materials and water are heated to be gelatinized; a prepared medium is subjected to oxygen-controlled fermentation and cultivation; activity of alpha-amylase is artificially regulated; and thus through the combined actions of the micro-organisms, enzymes and biocatalysts and through a series of complex biochemical reactions, polysaccharides, hyaluronic acid and other components keeping cell activity of animal and plant tissues and conducting repairing of damaged cells are finally formed. The formed fish bait preservative has uniquepreservation, antiseptic and anti-oxidation effects.
Owner:耿胜利

Method for preparing enzymes with smoked plum and turmeric as principal raw materials

Provided is a method for preparing enzymes with smoked plum and turmeric as principal raw materials. The method comprises the steps of (1) selecting, by weight, 35-45 parts of smoked plum, 10-15 parts of bananas, and 10-15 parts of pineapples, and cleaning the materials with clean water to be standby application; (2) firstly, squeezing the peel of the smoked plum to expose the pulp, cutting the pineapples and the bananas with peel into pineapple slices and banana slices both with a thickness of 0.3-2 centimeters; then mixing the materials evenly to obtain a mixture; (3) putting the mixture in a sealed container, adding honey and water into the mixture and mixing the new mixture evenly, wherein the weight ratio of the mixture, the honey and the water is 3:1:10; then putting the new mixture in a room under the normal temperature to be subjected to sealed fermentation, in the first 5-7 days, stirring the new mixture once every day, afterwards stirring the new mixture once every 7 days, keeping doing so for one month; (4) cleaning and cutting fresh turmeric dug around the time of the winter solstice into turmeric slices with a thickness of 0.3-2 centimeters, measuring, by weight, 15-25 parts of the turmeric slices and adding the turmeric slices into the sealed container to be subjected to sealed fermentation, the enzymes are obtained after above three months. According to the method for preparing enzymes with smoked plum and turmeric as principal raw materials, the obtained enzymes retain functions of original food, are rich in probiotics, and have abundant nutrition, good taste and a long shelf life.
Owner:唐妙红

Enzyme spectrum method for determining two-dimensional spatial distribution of activity of aquatic plant rhizosphere phosphatase

The invention relates to an enzyme spectrum method for determining two-dimensional spatial distribution of activity of aquatic plant rhizosphere phosphatase, which comprises the following steps of constructing an enzyme spectrum imaging system, drawing an enzyme spectrum calibration curve, and actually determining two-dimensional distribution of activity of aquatic plant rhizosphere acid and alkali phosphatase in sediments.The original state of phosphatase in the sediment is kept, the in-situ activity of the aquatic plant phosphatase in the sediment can be reflected more truly, polyacrylamide gel is used as a diffusion layer, transverse diffusion of a substrate and a fluorescent marker in the gel can be reduced by reducing the thickness of the membrane, and the spatial resolution is improved.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY

Double-electrolytic-cell device for micro-area electrochemical test and using method of double-electrolytic-cell device

The invention provides a double-electrolytic-cell device for micro-area electrochemical test and a using method thereof. The device comprises a hydrogen charging mechanism, a micro-area electrochemical testing mechanism and a gas collecting mechanism, and the hydrogen charging mechanism comprises a hydrogen charging cell, an observation mirror, a liquid inlet, a top plate, a hydrogen charging hole, an auxiliary electrode, a reference electrode, a lower sealing ring and an upper sealing ring; a sample is arranged between the lower sealing ring and the upper sealing ring, a hydrogen filling solution is loaded in the hydrogen charging cell, and the hydrogen filling solution is in contact with the lower surface of the sample; the micro-area electrochemical testing mechanism comprises an electrochemical workstation, a detection pool, a detection hole, a micro reference electrode and a platinum electrode probe; the detection pool is fixed on the top plate, and a hydrogen charging hole of thedetection pool is opposite to a detection hole of the detection pool; and when the sample is filled with hydrogen, the gas collecting mechanism discharges bubbles on the lower surface of the sample.According to the invention, a double-electrolytic-cell structure is adopted, preparation and installation processes of an experimental sample are simplified, hydrogen charging and in-situ micro-area electrochemical testing of the sample are realized, and application of the scanning electrochemical microscope in the field of hydrogen permeation research is expanded.
Owner:HARBIN INST OF TECH

Characterization method for porous structure of regenerated cellulose material

The invention discloses a characterization method for a porous structure of a regenerated cellulose material. The characterization method comprises the following steps: firstly, soaking regenerated cellulose into liquid and oscillating; secondly, carrying out nuclear magnetic relaxation hydrogen spectrum test on the regenerated cellulose treated in the first step; thirdly, carrying out inverse laplace transformation on a nuclear magnetic relaxation attenuation curve obtained by the nuclear magnetic relaxation hydrogen spectrum test to obtain parameters for characterizing the porous structure of the regenerated cellulose. According to the characterization method disclosed by the invention, the relationship that relaxation time (logarithms and horizontal coordinates) is in direct proportion to the content of pores (longitudinal coordinates) can be obtained, therefore, the sizes of the pores can be qualitatively explained; if specific values of the sizes of the pores need to be determined, different formulas are adopted for calculating according to difference of samples. According to the characterization method disclosed by the invention, drying or freeze-drying treatment of samples is not needed, so original states of the samples can be well kept.
Owner:INST OF CHEM CHINESE ACAD OF SCI

A kind of storage method of aerobic granular sludge

The embodiment of the invention discloses a method for storing aerobic granular sludge, which relates to the technical field of biological materials. The present invention adopts acetone aqueous solution to infiltrate the shaker with multiple gradients from low to high for the aerobic granular sludge to achieve the purpose of dehydration and drying. The inventor innovatively uses acetone to infiltrate and shake the bed to mix, which effectively solves the shortcomings of uneven dehydration in the existing aerobic granular sludge dry storage method. This method can protect microbial cells at different levels in similar levels. With a similar dehydration rate, it can effectively reduce the large structural damage of cells during drying and storage, and maintain the original state of the particles to a greater extent. Compared with the existing particle drying method, the present invention has simpler process.
Owner:SHANDONG NORMAL UNIV

Method for dissecting pupa brain of lepidopteran insect dead by freezing but with undamaged brain

The invention discloses a method for dissecting the pupa brain of a lepidopteran insect dead by freezing but with undamaged brain. The method comprises the following steps: after the skull of a pupa is cut off, the plasma in the skull is washed by cold physiological liquid, the brain in the skull of the pupa is fixed by fixing liquid after the brain is exposed, the membrane attached to the skull is clamped off, and then the formed brain is dissected out to obtain complete brain tissue. Compared with the prior art, the whole lepidopteran insect pupa brain can be obtained, the original state ofthe ultrastructure in the brain is completely maintained, structural damage is avoided, and the method is beneficial to obtaining of good results in a next experiment.
Owner:HENAN AGRICULTURAL UNIVERSITY

Porcine bronchial epithelial cell line, preparation method and application thereof

The invention provides a porcine bronchial epithelial cell line and a preparation method and application thereof, and belongs to the field of biological technology. The porcine bronchial epithelial cell line is hTERT-PBEC, and the preservation number is CCTCCNO:C201749. The preparation method of the cell line hTERT-PBEC includes constructing of eukaryotic expression vector pEGFP-hTERT carrying green fluorescent protein gene and human telomerase reverse transcriptase gene; using of Lipofectamin3000 for transfection of primary porcine bronchial epithelial cells with the eukaryotic expression vector pEGFP-hTERT, and G418 screening to obtain the porcine bronchial epithelial cell line hTERT-PBEC. The screening efficiency is remarkably improved through ingenious method, and the obtained porcine bronchial epithelial cell line can steadily pass over 60 generations, retains the characteristics of the primary cells, and can be applied to study on the pathogenic mechanism of porcine respiratory tract pathogen infection.
Owner:JIANGSU ACAD OF AGRI SCI

Cover plate culvert for pipeline protection and construction method thereof

The invention relates to a cover plate culvert for pipeline protection. The cover plate culvert comprises backfill original soil, a triangular truss, foam concrete, mounting points, isolation diaphragm walls, a pipeline, geogrid, geotextile and an impermeable membrane. The isolation diaphragm walls are arranged on the two sides of the pipeline, the mounting points are arranged at the tops of the isolation diaphragm walls, the triangular truss is arranged on the isolation diaphragm walls in a crossing mode through the mounting points, the foam concrete is arranged in the triangular truss, the geogrid, the geotextile and the impermeable membrane are sequentially arranged on the triangular truss filled with the foam concrete from bottom to top, and the backfill original soil is arranged abovethe impermeable membrane. The cover plate culvert has the beneficial effects that compared with the prior art, the soil engineering excavation amount and the fill amount are greatly reduced, an original state of a soil body around the pipeline is kept, and the technical advantages are remarkable; and the triangular truss and the foam concrete adopted by the cover plate culvert can obviously reduce the covering load of the upper part of the pipeline.
Owner:浙江世润建创科技发展有限公司

Device for sampling column shape samples of shallow water deposit

The invention discloses a sampling device for a column-shaped sample of shallow water sediment which includes a sampling tube component, a stress application component and a connector component arranged between the sampling tube component and the stress application component; the sampling tube component includes a sampling tube; the connector component includes a lower flange and a connecting piece connected by a bolt, a sealing piece arranged between the lower flange and the connecting piece, a connector connected on the connecting piece and a holding valve arranged in the connector and the connecting piece; the stress application component includes a plurality of hollow connecting rods connected on the connector and the top parts of the connecting rods are provided with ventilating screwed plug; the sampling tube passes through the lower flange and a lock ring, extents the connecting piece and is fixedly connected on the lower part of the connecting component through a bolt. By adopting the invention, the initial state of the column-shaped sample of the shallow water sediment can be maintained and good sampling effect is obtained. The sampling device has the advantages of compact structure, easy carry, convenient operation and being able to adapt various field sampling conditions.
Owner:CHINA INST OF WATER RESOURCES & HYDROPOWER RES

Special throwing type cold weapon for teaching

A special throwing cold weapon for teaching, which relates to the technical field of physical education teaching equipment, is composed of an outer shell, an inner shell, a cover, a connecting pipe, a holding tank and a support column, and an inner shell is arranged in the outer shell, and a holding container is arranged in the inner shell. The storage tank is provided with a connecting pipe and a support column, the connecting pipe is provided with a cover, and the cover is provided with a handle; the present invention has strong practicability, is very convenient to use, and has a very simple structure. The damage of cold weapons to the human body, and maintain the original state of throwing cold weapons, which is greatly conducive to the promotion of throwing cold weapons, so that people not only increase the fun when exercising, but also achieve a good training effect , to avoid the loss of traditional Chinese skills.
Owner:LUOYANG GONGHANG MECHANICAL TECH CO LTD

On-site undisturbed soil block sampling device easy to transport and small in disturbance and sampling method of on-site undisturbed soil block sampling device

PendingCN114414287AEase evaporationEnhanced original state sealing performanceWithdrawing sample devicesSoil scienceEnvironmental engineering
The invention discloses an on-site undisturbed soil block sampling device easy to transport and small in disturbance and a sampling method thereof, the sampling device is a totally-closed box body which is composed of a box cover, three box body plates, a soil cutting plate and a sealing plate and is provided with a cavity, a wrapping film is arranged in the totally-closed box body, and a dowel bar is arranged in the center of the top of the box cover. The sampling method comprises the following steps: determining a sampling area and making a corresponding mark; the sampling area is excavated and leveled, a sampling operation space and environment are formed, the sampling depth is determined, and the thickness of an undisturbed soil block sampling protection layer is reserved; an isolation groove is excavated around the sampling area, and the sampling area is separated from the surrounding soil body; a sampling device is adopted for sampling, the sampling device completing sampling is conveyed to an indoor laboratory, and the undisturbed soil blocks are taken out. A wrapping film and a sealing plate are arranged in the sampling device, so that evaporation of moisture in the original soil blocks during sampling, transportation and storage is relieved, and the original state sealing performance of the sampling device on the undisturbed soil blocks is enhanced.
Owner:LOGISTICAL ENGINEERING UNIVERSITY OF PLA

Original olive fruit powder and preparation method thereof

The present invention belongs to the technical field of food processing and specifically relates to original olive fruit powder and a preparation method thereof. The method comprises the following steps: sequentially blanching and enzyme deactivating olive fruits, and conducting vacuum freeze-drying, pitting and crushing to obtain the original olive fruit powder. The fruit powder prepared by the provided method retains original color and original flavor and reduces loss of nutrients. Results show the prepared fruit powder product is still olive green, while the color of the olive fruit powderobtained by a traditional olive fruit powder preparation method is changed.
Owner:GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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