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Method for increasing glycosaminoglycan stability of bioprosthetic heart valve

A valve glycosamine and stability technology, applied in the field of biomedical materials and medical devices, can solve the problems of ineffectiveness and low efficiency, and achieve the effects of prolonging service life, improving stability and improving anti-calcification performance.

Active Publication Date: 2019-06-04
VENUS MEDTECH (HANGZHOU) INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the previously reported method of adding neomycin sulfate has the limitation of low efficiency due to the use of carbodiimide crosslinking.
[0005] Therefore, by optimizing the chemical cross-linking method of biological heart valves, especially the development of new material processing methods that can improve the structural stability of glycosaminoglycans, the overall structural stability and anti-calcification performance of biological heart valves will be improved. The development of the industrial field is of great significance, but there is no good method at present, so it needs to be improved

Method used

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  • Method for increasing glycosaminoglycan stability of bioprosthetic heart valve
  • Method for increasing glycosaminoglycan stability of bioprosthetic heart valve
  • Method for increasing glycosaminoglycan stability of bioprosthetic heart valve

Examples

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Effect test

Embodiment 1

[0023] In this embodiment, the freshly collected porcine pericardium was washed with distilled water for 2 hours at 4°C and 100 RPM with shaking. Then soak in 50mM p-hydroxyphenylpropionic acid and 0.2M p-hydroxyphenylethylamine aqueous solution for 4 hours at room temperature. Then soak in 20 mM carbodiimide and 10 mM N-hydroxysuccinimide for 24 hours at room temperature. Simultaneously, p-hydroxyphenylpropionic acid-modified neomycin sulfate was prepared. Add p-hydroxyphenylpropionic acid and neomycin sulfate in an equimolar ratio, add 20mM carbodiimide and 10mM N-hydroxysuccinimide to react at room temperature for 24 hours, then add ethanol to precipitate and dialyze to clean, and prepare hydroxyphenylpropanoid Acid-modified neomycin sulfate. Add 0.4mM p-hydroxyphenylpropionic acid-modified neomycin sulfate to the above-mentioned hydroxyphenylpropionic acid and p-hydroxyphenylethylamine-modified pericardium, and then use 1% horseradish peroxidase and 1% hydrogen peroxide ...

Embodiment 2

[0025] In this embodiment, the freshly collected porcine pericardium was washed with distilled water for 2 hours at 4°C and 100 RPM with shaking. Then soak in 50mM p-hydroxyphenylpropionic acid and 0.2M p-hydroxyphenylethylamine aqueous solution for 4 hours at room temperature. Then soak in 20 mM carbodiimide and 10 mM N-hydroxysuccinimide for 24 hours at room temperature. Simultaneously, p-hydroxyphenylpropionic acid-modified neomycin sulfate was prepared. Add p-hydroxyphenylpropionic acid and neomycin sulfate in an equimolar ratio, add 20mM carbodiimide and 10mM N-hydroxysuccinimide to react at room temperature for 24 hours, then add ethanol to precipitate and dialyze to clean, and prepare hydroxyphenylpropanoid Acid-modified neomycin sulfate. Add 2 mM p-hydroxyphenylpropionic acid-modified neomycin sulfate to the above-mentioned hydroxyphenylpropionic acid and p-hydroxyphenylethylamine-modified pericardium, and then use 1% horseradish peroxidase and 1% hydrogen peroxide a...

Embodiment 3

[0027] In this embodiment, the freshly collected porcine pericardium was washed with distilled water for 2 hours at 4°C and 100 RPM with shaking. Then soak in 50mM p-hydroxyphenylpropionic acid and 0.2M p-hydroxyphenylethylamine aqueous solution for 4 hours at room temperature. Then soak in 20 mM carbodiimide and 10 mM N-hydroxysuccinimide for 24 hours at room temperature. Simultaneously, p-hydroxyphenylpropionic acid-modified neomycin sulfate was prepared. Add p-hydroxyphenylpropionic acid and neomycin sulfate in an equimolar ratio, add 20mM carbodiimide and 10mM N-hydroxysuccinimide and react at room temperature for 24 hours, then add ethanol to precipitate and dialyze to clean, and prepare hydroxyphenylpropanoid Acid-modified neomycin sulfate. Add 10 mM p-hydroxyphenylpropionic acid-modified neomycin sulfate to the above-mentioned hydroxyphenylpropionic acid and p-hydroxyphenylethylamine-modified pericardium, and then use 1% horseradish peroxidase and 1% hydrogen peroxide...

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Abstract

The invention discloses a method for increasing stability of bioprosthetic heart valve glycosaminoglycan. The method comprises the following processes: Modification of pig or bovine pericardium with p-hydroxyphenylpropionic acid or p-hydroxyphenylethylamine, modification of neomycin sulfate with p-hydroxyphenylpropionic acid, and initiation of enzyme cross-linking under conditions of horseradish peroxidase and hydrogen peroxide. A phenolic hydroxyl group can be introduced into the p-hydroxyphenylpropionic acid modified neomycin sulfate, and horseradish peroxidase and hydrogen peroxide will achieve chemical crosslinking of the phenolic hydroxyl group. The method provided by the invention can raise the glycosaminoglycan stability and anti-calcification performance of bioprosthetic heart valve and potentially prolong its service life.

Description

technical field [0001] The invention relates to the technical field of biomedical materials and medical devices, in particular to a method for improving the stability of glycosaminoglycans of biological heart valves. Background technique [0002] Bioprosthetic valves are usually prepared from pig or bovine pericardium and are used to replace dysfunctional human heart valves. Bioprostheses currently used in clinical practice are usually prepared by glutaraldehyde cross-linking. Bioprosthetic valves prepared with glutaraldehyde still have some disadvantages. Bioprosthetic valves prepared with glutaraldehyde can effectively cross-link collagen but not glycosaminoglycans. [0003] Glycosaminoglycans, a type of heteropolysaccharides, mainly exist in the connective tissues of higher animals. There are glycosaminoglycans in human heart valves and pericardium, and its main function is to act as a buffer layer to reduce damage caused by valve stress. Uncrosslinked glycosaminoglyc...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王云兵雷洋
Owner VENUS MEDTECH (HANGZHOU) INC
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