Coriaria sinica maxim mushroom liquid strain preparation method

A technology of liquid strains and secondary liquid strains, which is applied in the field of bioengineering, can solve the problems of restricting the popularization and application of shiitake mushrooms, and long cultivation time, so as to shorten the seed production time, develop mycelium quickly, and strain strains easily. The effect of survival

Inactive Publication Date: 2019-06-07
贵州正黔门大健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, at present, there are few researches on the preparation method of the liquid strain of Matsutake mushrooms, and the traditional preparation method of the Matsutake mushroom strain is still used, and the cultivation time is long, which seriously restricts the popularization and application of Matsutake mushrooms.

Method used

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  • Coriaria sinica maxim mushroom liquid strain preparation method
  • Coriaria sinica maxim mushroom liquid strain preparation method

Examples

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Effect test

Embodiment 1

[0021] A kind of preparation method of liquid strain of shiitake mushroom, concrete steps are as follows:

[0022] (1) Using the tissue separation method, the strains were extracted from the fruiting body of Lentinus edodes under aseptic conditions, inoculated on the test tube culture medium, and cultured at 18°C ​​for 22 days to obtain the test tube parent species;

[0023] (2) Under aseptic conditions, use an inoculation needle to remove the 1.3-1.5 cm medium near the end of the test tube, cut the bacteria on the remaining medium to obtain bacterial blocks, and inoculate the bacterial blocks to contain the first-level liquid culture In the Erlenmeyer flask of the base, each Erlenmeyer flask was inoculated with 5 pieces; 18°C ​​was left standing for 72h; the standing Erlenmeyer flask was placed on a shaker, and shaken for 72h to obtain a first-class liquid strain;

[0024] (3) it is 0.04MPa to be inoculated to pressure under aseptic condition by the inoculum size of 3% by the...

Embodiment 2

[0027] A kind of preparation method of liquid strain of shiitake mushroom, concrete steps are as follows:

[0028] (1) Using the tissue separation method, the strains were extracted from the fruiting body of Lentinus edodes under aseptic conditions, inoculated on the test tube culture medium, and cultured at 22°C for 18 days to obtain the test tube parent species;

[0029] (2) Under aseptic conditions, use an inoculation needle to remove the 1.3-1.5 cm medium near the end of the test tube, cut the bacteria on the remaining medium to obtain bacterial blocks, and inoculate the bacterial blocks to contain the first-level liquid culture In the Erlenmeyer flask of base, each Erlenmeyer flask was inoculated with 8 pieces; 22°C was left standing for 48h; the standing Erlenmeyer flask was placed on a shaker, shaken and cultured for 48h, and the first-grade liquid strain was obtained;

[0030] (3) the one-level liquid bacterial classification that step (2) obtains is by the inoculum si...

Embodiment 3

[0033] A kind of preparation method of liquid strain of shiitake mushroom, concrete steps are as follows:

[0034] (1) Using the spore separation method, the strains were extracted from the fruiting body of Lentinus edodes under aseptic conditions, inoculated on the test tube culture medium, and cultivated at 20°C for 20 days to obtain the test tube parent species;

[0035] (2) Under aseptic conditions, use an inoculation needle to remove the 1.3-1.5 cm medium near the end of the test tube, cut the bacteria on the remaining medium to obtain bacterial blocks, and inoculate the bacterial blocks to contain the first-level liquid culture In the base Erlenmeyer flask, each Erlenmeyer flask was inoculated with 6 pieces; 20°C was left standing for 60h; the standing Erlenmeyer flask was placed on a shaker, and shaken and cultured for 60h to obtain a first-class liquid strain;

[0036] (3) it is 0.04MPa to be inoculated to pressure under aseptic condition by the inoculum size of 4% by ...

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Abstract

The invention discloses a coriaria sinica maxim mushroom liquid strain preparation method which includes the steps: extracting a strain from a coriaria sinica maxim mushroom sporocarp under aseptic conditions by a tissue isolation or spore isolation method, and inoculating the strain onto a test tube medium to obtain a test tube mother strain; inoculating the test tube mother strain into a triangular flask containing a primary liquid medium under the aseptic conditions and performing shaking culture to obtain a primary liquid strain; inoculating the primary liquid strain into a fermentation tank containing a secondary liquid medium under the aseptic conditions to obtain a secondary liquid strain; discharging the secondary liquid strain to an inoculation barrel containing a secondary liquidmedium under the aseptic conditions for standby application. Compared with a traditional coriaria sinica maxim mushroom strain preparation method, the coriaria sinica maxim mushroom liquid strain preparation method has the advantages that strain production time can be greatly shortened, during cultivation and inoculation, inoculation is rapid, working efficiency is high, the strain easily survives, permeates and grows, hyphal development is rapid and the like.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing a liquid strain of shiitake mushrooms. Background technique [0002] Masang mushroom (LentinusedodesSing.), also known as Masang fungus, Wulixiang; born in the dead branches of Masang in spring and autumn. Masang mushroom is the only species found in the genus Lentinus that grows on the Masang tree. At present, the aroma genetic genes of other shiitake mushrooms have been lost during the long-term cultivation process, resulting in the lack of strong aroma of these shiitake mushrooms. Masang mushrooms are delicious and fragrant, rich in nutrients, and have great development and utilization value. [0003] However, at present, there are few studies on the preparation method of liquid strains of S. edodes, and the traditional preparation method of S. edodes is still used, and the cultivation time is long, which seriously restricts the popularization an...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/00
Inventor 周利
Owner 贵州正黔门大健康产业有限公司
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