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A method for preparing Chinese medicine extract with antiviral effect by high-speed countercurrent chromatography

A technology of high-speed countercurrent chromatography and antiviral effect, which is applied in the direction of antiviral agents, pharmaceutical formulas, and medical preparations containing active ingredients, etc. It can solve the problems of few research reports on Dingguicao and achieve remarkable results

Active Publication Date: 2021-10-22
珠海希望基因医药研究院有限公司
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Problems solved by technology

[0003] At present, there are not many research reports on the active ingredients in Dingguicao that have antiviral effects in the existing literature.
However, Dingguicao contains many chemical components, and which effective parts have antiviral effects need to be further researched and developed by researchers; in addition, because there are many types of viruses, whether the effective parts in Dingguicao are effective against all viruses Whether it has antiviral effect, or has antiviral effect on some viruses, this still needs research and development personnel to conduct research and tackle key problems through a large number of experiments

Method used

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  • A method for preparing Chinese medicine extract with antiviral effect by high-speed countercurrent chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The preparation of embodiment 1 Chinese medicine extract

[0027] Take the Chinese herbal medicine Dingguicao, heat and reflux extract once with ethanol with a volume fraction of 70%, and the extraction time is 1.5h; concentrate the extract to remove the solvent to obtain the Dingguicao alcohol extract; wherein the Chinese medicinal material Dingguicao and ethanol aqueous solution The dosage ratio is 1g: 15mL;

[0028] (2) Add water to suspend the bucuro alcohol extract, then extract twice with chloroform and ethyl acetate successively, combine the ethyl acetate extraction parts obtained by the two extractions, concentrate and remove the ethyl acetate solvent, and obtain ethyl acetate Ester extract; Wherein the consumption ratio of butyricol extract and water is 1g:20mL; The volume consumption ratio of the volume consumption of chloroform and ethyl acetate and water in each extraction process is 1:1;

[0029] (3) The ethyl acetate extract is separated by high-speed cou...

experiment example

[0055] Experimental example: Antiviral activity test of traditional Chinese medicine extracts

[0056] Take a 96-well cell culture plate covered with a monolayer of MDCK cells, discard the supernatant, wash twice with PBS, add 100TCID 50 The virus solution was 100 μL / well, adsorbed for 1 h, and then 100 μL of the drug solution diluted with MEM was added to each well, and a cell control group, a virus control group, and a blank control group were set up. Placed at 37°C, saturated humidity, 5% CO 2 Incubate in the incubator. After 48 hours, the old solution in the 96-well plate was discarded, and the original solution of CCK8 was diluted ten times, and 100 μL per well was added to the wells to be tested. After incubating in the incubator for 1 h, measure the absorbance at 450 nm with a microplate reader, and calculate the IC at the same time 50 . The test results are shown in Table 1. In the test results, IC 50 The smaller the value, the better the anti-herpes virus effect....

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Abstract

The invention relates to the technical field of traditional Chinese medicine, and specifically discloses a method for preparing a traditional Chinese medicine extract with antiviral effect by utilizing high-speed countercurrent chromatography technology. The method comprises the following steps: (1) taking the Chinese herbal medicine Dingguicao, extracting it with alcohol, concentrating the extract to remove the solvent to obtain the Dingguicao alcohol extract; (2) adding water to suspend the Dingguicao alcohol extract, and then Sequentially extract 1 to 3 times with chloroform and ethyl acetate respectively, take the extraction part of ethyl acetate, concentrate and remove the ethyl acetate solvent, and obtain the ethyl acetate extract; (3) process the ethyl acetate extract by high-speed countercurrent chromatography Separated to obtain the described Chinese medicine extract. The Chinese medicinal herb extract prepared by the method has anti-respiratory syncytial virus and influenza virus effects.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for preparing a traditional Chinese medicine extract with antiviral effect by utilizing high-speed countercurrent chromatography technology. Background technique [0002] Dingguicao, the name of Chinese medicinal materials, is the whole herb of the leguminous plant Dingguicao. Harvested in summer, the functions and indications are: heat clearing, detoxification, blood stasis removal, cold, high fever convulsions, diarrhea, jaundice, dysentery, malnutrition in children, sore throat, red eyes, boils and swollen toxins, mastitis. [0003] At present, there are not many research reports on the active ingredients in Dingguicao that have antiviral effects in the existing literature. However, Dingguicao contains many chemical components, and which effective parts have antiviral effects need to be further researched and developed by researchers; in addition...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P31/14A61P31/16
Inventor 曹文强杨懋勋王重庆刘荫贞赵柏松
Owner 珠海希望基因医药研究院有限公司
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