Zanthoxylum schinifolium Sieb et Zucc oil quality control method
A quality control method, the technology of rattan pepper oil, applied in color/spectral characteristic measurement, food testing, material inspection, etc. There are no obvious distinguishing indicators for oily aroma and numbness, etc., to achieve the effect of simple quality control methods and obvious changes in aroma and numbness
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Embodiment 1
[0043] A quality control method for rattan pepper oil, including detection of fragrance index and detection of numbness index;
[0044] Detection of aroma index: determination of volatile oil content X in rattan pepper oil;
[0045] Detection of numbness index: Determination of pepperonamide content C in rattan pepper oil;
[0046] The assay method of pepperonamide content C in rattan pepper oil comprises the following steps:
[0047] a, prepare the pepper amide standard substance, the prepared pepper amide standard substance is mixed with solutions of different concentrations, the absorbance value of each concentration sample solution at 270nm is the ordinate, the concentration is the abscissa, and the standard curve is drawn;
[0048] b. Prepare rattan pepper oil samples: take rattan pepper oil, add methanol, and ultrasonicate for 5 minutes in a water bath at 30°C, transfer the extract into a centrifuge tube, and centrifuge for 10 minutes at a speed of 2500r / min; take the s...
Embodiment 2
[0060] Based on Example 1, the described method for quality control of a kind of rattan pepper oil also includes fragrance classification and numbness classification;
[0061] Aroma classification: according to the volatile oil content X in the rattan pepper oil, the rattan pepper oil aroma is classified;
[0062] Grading of numbness: According to the content C of prickly amide in rattan pepper oil, the rattan pepper oil is graded for numbness.
[0063] The invention determines the aroma and hemp index of rattan pepper oil by detecting the content of volatile oil and pepper amide, and grades the aroma and hemp indexes of rattan pepper oil, so that consumers and producers can clearly understand the product Fragrance and numbness. The present invention determines the corresponding volatile oil content and prickly amide content when the sense of numbness changes significantly through classification, thereby systematically clarifying the grading standard of rattan pepper oil, and...
Embodiment 3
[0067] Based on embodiment 1, described a kind of rattan pepper oil quality control method, the assay method of volatile oil content X in rattan pepper oil comprises the following steps:
[0068] A, weighing rattan pepper oil;
[0069] B. Add distilled water and explosion-proof glass beads to start distillation;
[0070] C. Read the volume of volatile oil;
[0071] D. Calculate the volatile oil content X in the rattan pepper oil with the volume of the volatile oil and the quality of the rattan pepper oil sample.
[0072] The formula for calculating the volatile oil content X is:
[0073] In the formula:
[0074] X - volatile oil content, in milliliters per hectogram (ml / 100g);
[0075] V—the volume of volatile oil in rattan pepper oil measured from the distillation receiving tube, in milliliters (ml);
[0076] m—sample mass, in grams (g);
[0077] Test the precision of the measured value: the absolute difference of two independent measurement results obtained under rep...
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