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Application of bergamottin and/or casticin and product applying same

A technology of vitexin and bergamot can be applied in the field of medicine, which can solve the problems such as the urgent development of antiviral drugs and the lack of treatment of arenavirus, and achieve the effects of good prevention and treatment and inhibition of infection.

Active Publication Date: 2019-06-11
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no specific drug for the treatment of arenaviruses, therefore, the development of antiviral drugs is urgent

Method used

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  • Application of bergamottin and/or casticin and product applying same
  • Application of bergamottin and/or casticin and product applying same
  • Application of bergamottin and/or casticin and product applying same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] By transfecting a plasmid with vesicular stomatitis virus (VSV) gene and transfecting a plasmid containing LASV envelope glycoprotein (GenBankaccession number HQ688673.1), the envelope protein is constructed as arenavirus LASV envelope glycoprotein, which contains a report Genetic recombinant virus, while simulating the virus infection process, can conveniently and timely perform high-throughput screening and detection. Ten concentrations of bergamot and vitexin were selected (diluted starting from 25 μM) to treat Vero cells and A549 cells for 1 hour, and then LASV infected the cells with MOI=0.1 for 1 hour (without LASV envelope sugar Protein VSVpv infected Vero cells as a control group), the liquid was changed and the mixture of drug and virus was discarded, and after 23 hours, the cells were lysed to determine the luciferase reading value. The results of bergamotin inhibition of LASV are as follows figure 1 As shown, the results of vitexin inhibiting LASV are as fol...

Embodiment 2

[0064] With reference to the test method in Example 1, construct recombinant viruses of other viruses of the Arenaviridae family, and the Genebank numbers are as follows: LUJV (NC_012776.1), MOPV (AY772170.1), GTOV (NC_005077.1), JUNV (strain XJ13, NC_005081.1), MACV (strain Carvallo, NC_005078.1), SABV (U41071.1), CHPV (NC_010562.1), EBOV (strain Mayinga, EU224440.2), and MARV (YP_001531156.1).

[0065] With reference to the test operation in Example 1, the inhibitory effect of bergamot on the above-mentioned Arenaviridae virus is detected, and the results are as follows: image 3 (GTOVpv, IC 50 = 1.516μM), Figure 4 (JNUVpv, IC 50 =3.374μM), Figure 5 (MACVpv, IC 50 = 11.7μM), Figure 6 (SABVpv, IC 50 = 3.861μM), Figure 7 (CHPVpv, IC 50 =5.876μM), it can be seen that bergamot also has inhibitory effect on JUNV, MACV, GTOV, CHPA and SABV infection.

[0066] With reference to the test operation in Example 1, detect the inhibitory effect of vitexin to the above-mentio...

Embodiment 3

[0068] The cytotoxicity of bergamot and vitexin was detected, and the cell viability was measured by MTT method.

[0069] MTT can be reduced by intracellular mitochondrial succinate dehydrogenase to form crystalline formazan, which is insoluble in water but readily soluble in DMSO. After dissolving in DMSO, the absorbance at 492nm can be detected with a microplate reader to quantify this substance. The activity of succinate dehydrogenase reflects the activity of cells, so the detection of the amount of formazan can reflect the activity of cells. Three concentrations of bergamot and vitexin were respectively set at 100 μM, 50 μM, and 25 μM. The specific steps of the method are as follows:

[0070] 1) Spread Vero cells into a 96-well plate with a cell density of 1.0×10 5 individual / mL.

[0071] 2) Add serially diluted inhibitors bergamotin or vitexin (diluted in DMEM medium containing 2% FBS) to the cells and incubate for 24 hours.

[0072] 3) Discard the cell supernatant, a...

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Abstract

The invention relates to the technical field of medicines, and particularly provides application of bergamottin and / or casticin and a product applying the same. The invention provides application of the bergamottin and / or casticin in preparation of a product for preventing and / or treating Arenaviridae virus infection. The bergamottin and casticin are both traditional Chinese medicine monomer smallmolecule compounds, can effectively inhibit the infection of cells by Arenaviridae virus in a non-toxic range, and fill the blank of lack of Arenaviridae virus infection treatment at present. Products prepared by adopting the bergamottin and / or casticin as active ingredients comprise medicines and functional foods, and have good effects on prevention and treatment of arenavirus infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an application of bergamot and / or vitexin and a product using the same. Background technique [0002] The Arenaviridae Mammalian Arenavirus genus includes more than 30 species of viruses, which can be divided into Old World virus and New World virus according to virus antigenicity, serotype and geographical distribution. Both Lassa virus (LASV) and Lujo virus (LUJV) in the old world cause severe infectious hemorrhagic disease with a high fatality rate, and are listed as Class A bioterrorism pathogens by the US CDC. The pathogenic viruses in the mammalian arenavirus genus also include the New World Junín virus (JUNV), Machupo virus (Machupo virus, MACV), Guanarito virus (Guanarito virus, GTOV), sand Biya virus (Sabiávirus, SABV), Chaparevirus (Chaparevirus, CHPA). The above-mentioned viruses, like Lassa virus, are level 4 pathogens and pose a huge threat to human safety. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61K31/352A61P31/14A23L33/105
Inventor 王薇肖庚富刘洋郭娇贾潇瑛曹浚垣张光顺王培林
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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