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Method for quickly proliferating stem of sterile lotus seedlings

A technology for sterile seedlings and lotus flowers, applied in the field of plant tissue culture, can solve the problems of low survival rate, high pollution rate, low proliferation efficiency, etc. Effect

Active Publication Date: 2019-06-14
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a construction method for the rapid proliferation of aseptic lotus seedlings through stems, so as to solve the problems that the cultivation process of the prior art is not easy to produce stems, the proliferation efficiency is low, the pollution rate is high, and the survival rate is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for constructing rapid multiplication of lotus aseptic seedlings through stems, which is carried out in the following steps:

[0039] Taking the first-generation tissue cultured seedlings of red lotus in Weishan Lake as materials: select ms as the basic medium, set up 4 experimental groups, set up 4 experimental groups, use two kinds of culture methods and add two kinds of sucrose content medium respectively: solid Liquid culture: Experimental group 1: MS+6-BA 1.0mg / L+IBA0.5mg / L+GA3mg / L+9.5g / L agar+60g / L sucrose, pH=5.8; Experimental group 2: MS+6- BA 1.0mg / L+IBA0.5mg / L+0.05GA3mg / L+9.5g / L agar+30g / L sucrose, pH=5.8; solid culture: Experimental group 3: MS+6-BA 1.0mg / L+IBA0 .5mg / L+GA3mg / L+9.5g / L agar+60g / L sucrose, pH=5.8; experimental group 4: MS+6-BA 1.0mg / L+IBA0.5mg / L+0.05GA3mg / L+9.5 g / L agar + 30g / L sucrose, pH=5.8; put it into a 120°C high-temperature sterilization pot for high-temperature sterilization after preparation, take it out after sterilization, s...

Embodiment 2

[0052] A method for constructing rapid multiplication of lotus aseptic seedlings through stems, which is carried out in the following steps:

[0053] Taking the first-generation tissue-cultured seedlings of red lotus in Weishan Lake as the material: select ms as the basic medium, set up 4 experimental groups, use two culture methods and add two kinds of sucrose content medium respectively: 1. Solid-liquid culture: experiment Group 1: MS+6-BA1.0mg / L+IBA0.5mg / L+GA3mg / L+9.5g / L agar+60g / L sucrose, pH=5.8; Experimental group 2: MS+6-BA1.0mg / L L+IBA0.5mg / L+0.05GA3mg / L+9.5g / L agar+30g / L sucrose, pH=5.8; 2. Solid culture: Experimental group 3: MS+6-BA 1.0mg / L+IBA0.5mg / L+GA3mg / L+9.5g / L agar+60g / L sucrose, pH=5.8; experimental group 4: MS+6-BA 1.0mg / L+IBA0.5mg / L+0.05GA3mg / L+9.5g / L agar + 30g / L sucrose, pH=5.8, put it into a 120°C high-temperature sterilization pot for high-temperature sterilization after preparation, take it out after sterilization, shake the culture bottle gently to...

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PUM

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Abstract

The invention discloses a method for quickly proliferating stem of sterile lotus seedlings. The method comprises the following steps: selecting green sterile seedling buds of lotus petiole and leaves,inoculating a sterile proliferation culture medium under a sterile condition, adding sterile water of 1-2 cm, sealing and putting into a tissue culture chamber, wherein the illumination time per dayis 14-16 hours, and the illumination intensity is 1500-2500 Lx; controlling temperature at 25-28 DEG C, culturing for 40-45 days to obtain a large number of stem-growing seedlings, adding 6-BA 1-1.5 mg / L+IBA 0.5-1 mg / L+GA 3.0.05-0.08 mg / L+sucrose 60 g / L, agar 9.5 g / L, and pH=5.8. According to the method, the efficiency for generating stem-growing seedlings and the survival rate of the seedlings are obviously improved, and the pollution rate, the loss rate and the yellowing rate are reduced to the lowest.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for rapid multiplication of lotus aseptic seedlings through stems. Background technique [0002] The lotus (Nelumbo nucifera Gaertn.) is a perennial aquatic flower of the genus Nelumbo of the family Nelumbo. It is one of the ten traditional famous flowers in China. It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. [0003] The method of propagation and preservation of lotus mainly adopts traditional split lotus root and large-area tank planting or pond planting. However, conventional methods of propagation not only have a low reproductive coefficient and a large amount of seeds, but also cause severe plant yield reduction and poor quality due to frequent occurrence of viral diseases and other diseases. This problem has become a major obstacle restricting the development of lotus planting. The method o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 徐迎春王珍董相明金奇江王彦杰
Owner NANJING AGRICULTURAL UNIVERSITY