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Composite microbial bacterial fertilizer, and preparation method and application thereof

A technology of compound microbial bacteria and live bacteria, applied in the direction of organic fertilizers, can solve the problems of beneficial biological stress, control failure, difficult degradation, etc., and achieve the effect of promoting yield increase and good control effect.

Inactive Publication Date: 2019-06-14
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, for the control of angular spot disease of persimmon trees, chemical pesticide fungicides are still the main method, and the environmental pollution problem caused by the large-scale use of chemical pesticides is also becoming more and more serious; Drug resistance and drug resistance increase the difficulty of prevention and control, and even cause the failure of prevention and control
In addition, chemical pesticides can pose a threat to beneficial organisms because they are not selective; moreover, many chemical pesticides are non-degradable or difficult to degrade, and are easy to accumulate in animals and plants, resulting in the enrichment of pollutants

Method used

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  • Composite microbial bacterial fertilizer, and preparation method and application thereof
  • Composite microbial bacterial fertilizer, and preparation method and application thereof
  • Composite microbial bacterial fertilizer, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of compound microbial bacterial fertilizer, the specific steps are as follows:

[0030] (1) prepare the live bacteria powder of Bacillus subtilis and Burkholderia cepacia

[0031] a. Strain cultivation: transfer the preserved Bacillus subtilis and Burkholderia cepacia respectively to the test tube slant made of freshly prepared solid medium to activate the strains;

[0032] b. Seed tank cultivation: add liquid culture medium in the seed tank, and cultivate the activated strain in the seed tank;

[0033] c. Fermentation tank culture: when the number of Bacillus coagulans in the culture solution in the seed tank reaches 2x10 9 At cfu / ml, the culture solution in the seed tank was transferred to 1m with a 10% inoculum 3 In the liquid culture medium of the fermenter, maintain 30°C, tank pressure 0.04MPa, carry out deep aeration and stirring culture for 24h;

[0034] d. Preparation of Bacillus subtilis and Burkholderia cepacia live bacteria powder: tra...

Embodiment 2

[0043] A preparation method of compound microbial bacterial fertilizer, the specific steps are as follows:

[0044](1) prepare the live bacteria powder of Bacillus subtilis and Burkholderia cepacia

[0045] a. Strain cultivation: transfer the preserved Bacillus subtilis and Burkholderia cepacia respectively to the test tube slant made of freshly prepared solid medium to activate the strains;

[0046] b. Seed tank cultivation: add liquid culture medium in the seed tank, and cultivate the activated strain in the seed tank;

[0047] c. Fermentation tank culture: when the number of Bacillus coagulans in the culture solution in the seed tank reaches 3x10 9 At cfu / ml, the culture solution in the seed tank was transferred to 1m with a 5% inoculum 3 In the liquid culture medium of the fermenter, maintain 37°C, tank pressure 0.06MPa, carry out deep aeration and stirring culture for 30h;

[0048] d. Preparation of Bacillus subtilis and Burkholderia cepacia live bacteria powder: trans...

Embodiment 3

[0057] A preparation method of compound microbial bacterial fertilizer, the specific steps are as follows:

[0058] (1) prepare the live bacteria powder of Bacillus subtilis and Burkholderia cepacia

[0059] a. Strain cultivation: transfer the preserved Bacillus subtilis and Burkholderia cepacia respectively to the test tube slant made of freshly prepared solid medium to activate the strains;

[0060] b. Seed tank cultivation: add liquid culture medium in the seed tank, and cultivate the activated strain in the seed tank;

[0061] c. Fermentation tank culture: when the number of Bacillus coagulans in the culture solution in the seed tank reaches 2x10 9 At cfu / ml, the culture solution in the seed tank was transferred to 1m with a 10% inoculum 3 In the liquid medium of the fermenter, maintain 35°C, tank pressure 0.05MPa, carry out deep aeration and stirring culture for 27h;

[0062] d. Preparation of live bacterial powders of Bacillus subtilis and Burkholderia cepacia: transf...

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Abstract

The invention discloses a composite microbial bacterial fertilizer, and a preparation method and application thereof. The composite microbial bacterial fertilizer is prepared from bacillus subtilis, burkholderia cepacia and the bacillus licheniformis according to the weight ratio of (1 to 2):(1 to 2):(1 to 3) through compounding; the bacterial concentration of each of the bacillus subtilis, the burkholderia cepacia and the bacillus licheniformis is 6*10<9>cfu / g. The composite microbial bacterial fertilizer has the good prevention and treatment effects on persimmon angular leaf spot; no chemical residue is left; drug resistance cannot be easily generated; the yield of persimmon trees can also be promoted to be increased by 20 percent or above.

Description

technical field [0001] The invention relates to the technical field of fertilizer production, in particular to a compound microbial fertilizer and its preparation method and application. Background technique [0002] Persimmon angular spot disease belongs to the subphylum Deuteromycota, and belongs to the subphylum Pyrospora, which damages persimmon leaves and persimmon pedicles; from May to August, the rainfall is early, the rainy days are many, and the rainfall is large, which is conducive to the production and invasion of conidia, and the onset is early. Seriously, the old leaves, the lower leaves of the crown and the inner chamber leaves are severely affected; the leaves are damaged, and irregular yellow-green lesions appear on the front at the beginning, and then the color gradually deepens, turning brown or dark brown, and the lesions are densely covered with small black fluffy granules point. The disease of persimmon pedicles mostly occurs around the pedicles, brown ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 余贤美艾呈祥王洁张坤鹏马亚男
Owner SHANDONG INST OF POMOLOGY