Goat HIAT1 gene insertion/deletion polymorphism detection method and application thereof

Abstract

The invention discloses a goat HIAT1 gene insertion / deletion polymorphism detection method and application thereof. A whole genome DNA of a to-be-detected goat serves as a template, and a goat HIAT1 gene part fragment is amplified through a PCR; agarose gel electrophoresis is carried out, and the genotypes of 15-bp insertion / deletion polymorphism loci at goat HIAT1 gene NC_030810.1:g.7695-7696 bits are identified through electrophoresis results. Related analysis results show that different genotypes of goat HIAT1 gene 15-bp insertion / deletion polymorphism have a remarkable correlation with growth characters of Shannxi white cashmere goats and can serve as DNA markers for improving the growth characters of the goats. The goat HIAT1 gene insertion / deletion polymorphism detection method can be applied to goat molecular marker assisted selective breeding so as to accelerate the establishment of a goat genetic resource group with excellent growth characters.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the rapid and accurate typing detection of goat HIAT1 gene NC_030810.1:g.7695-7696 15-bp insertion / deletion polymorphism (indel) site and its molecular marker Applications in assisted selection breeding. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of molecular breeding technology system, molecular marker-assisted selection (MAS) breeding technology is widely used in actual breeding work. MAS breeding technology can quickly and accurately analyze the genetic composition of individuals at the DNA level, transfer the target gene to the parent that needs to be improved through hybridization, and combine the identification of the target genotype with traditional breeding to achieve dire...

AI Technical Summary

Problems solved by technology

A study has re-sequenced the whole goat genome and found that the HIAT1 gene is significantly different in meat goats compared with cashmere and dairy goats (Zhang B et al., 2017). Reports of indel loci and their significant correlation with growth and development traits

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