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Three-dimensional curved surface reconstruction method suitable for biological membrane

A three-dimensional curved surface and surface reconstruction technology, applied in the field of biology, can solve problems such as limited thickness, inability to obtain ideal characterization results, inability to penetrate dense biofilm tissue, etc., and achieve a high degree of reduction effect

Active Publication Date: 2019-06-14
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for biofilms with complex three-dimensional structures and thick biofilms, the characterization of their physical forms has always been one of the problems that plague the majority of researchers.
Scholars at home and abroad use confocal laser microscopy (CLSM) to measure the three-dimensional structure of biofilms. The limited amount of light transmitted cannot penetrate the dense biofilm tissue, so ideal characterization results cannot be obtained

Method used

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  • Three-dimensional curved surface reconstruction method suitable for biological membrane
  • Three-dimensional curved surface reconstruction method suitable for biological membrane
  • Three-dimensional curved surface reconstruction method suitable for biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] (1) Making biofilm slices

[0078] (1) Cleaning and sampling

[0079] Clean the biofilm carrier with PBS to remove the nutrient solution contained on its surface and inside, and use tools to scrape the biofilm into the petri dish;

[0080] (2) Fixed

[0081] Fix the biofilm sample in 2.5v / v% glutaraldehyde solution, and place it at 4°C for 24h;

[0082] (3) rinse

[0083] Take out the sample, absorb the excess fixative on the surface with absorbent paper, rinse 3 times with 0.2mol / L, pH7.2 PBS phosphate buffer solution;

[0084] (4) Dehydration

[0085] The samples were dehydrated with 50%, 75%, 85%, 90% and 95% ethanol solutions for 15 minutes; then treated with 100% ethanol twice, 20 minutes each time;

[0086] (5) Permeabilization

[0087] Use 0.5v / v% TrixonX-100 / PBS solution to stand at 4°C for 10 minutes to increase the permeability of the cell membrane, and wash with 0.2mol / L PBS phosphate buffer solution for 3 times;

[0088] (6) Waxing and embedding

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Abstract

The invention relates to a three-dimensional curved surface reconstruction method suitable for a biological membrane, and belongs to the technical field of biology. The main technical scheme is as follows: cleaning and sampling, fixing, rinsing, dehydrating, permeabilizing, waxing, embedding, slicing, sticking and baking, dewaxing, dyeing and sealing; adjusting the wavelength of emitted light, adjusting the amplification factor, photographing and storing images; and building a three-dimensional model. The three-dimensional curved surface reconstruction method suitable for the biological membrane provided by the invention is proposed for a biological membrane with the thickness greater than 0.1 mm, and in order to avoid the problem that a dye and a laser light beam cannot effectively enterthe interior of the biological membrane due to the fact that the biological membrane is excessively thick, thus the characterization is difficult. By using the technology, the complex physical form ofthe biological membrane can be effectively reconstructed, meanwhile, a relatively high reduction degree is ensured, and a feasible means is provided for researching the form change of the biologicalmembrane and influence factors.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a method for reconstructing three-dimensional curved surfaces applicable to biological membranes. Background technique [0002] Biofilm is one of the main forms of microbial aggregation. Biofilm is a layered aggregate formed by microorganisms and extracellular polymers attached to a solid surface, with a certain thickness and complex three-dimensional shape. Biofilms are ubiquitous in nature, but human research on biofilms began with biofilms attached to reactors and carriers in biofilm wastewater treatment processes. A biofilm system usually consists of four parts: bulk liquid, boundary layer, biofilm and substrate, such as figure 1 shown. The formation of biofilm includes two processes of microbial attachment and immobilization on the carrier surface, which are affected by factors such as fluid flow, substrate type and temperature. Biofilm not only has a complex three-dimen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/30G01N1/36G01N1/28G01N1/34G06T17/20
Inventor 韩越梅
Owner DALIAN UNIV
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