Three-dimensionally cultured mesenchymal stem cells derived exosomes and preparation method and application thereof

A technology of stem cells and three-dimensional culture, applied in the field of biomedical materials, can solve the problem of no three-dimensional scaffolding materials, and achieve the effects of improving memory and cognitive ability, increasing production, and inhibiting amyloid deposition.

Inactive Publication Date: 2019-06-21
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on whether three-dimensional scaffold materials can regulate the function of exosomes derived from mesenchymal stem cells and affect the treatment of Alzheimer's disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensionally cultured mesenchymal stem cells derived exosomes and preparation method and application thereof
  • Three-dimensionally cultured mesenchymal stem cells derived exosomes and preparation method and application thereof
  • Three-dimensionally cultured mesenchymal stem cells derived exosomes and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0048] Another aspect of the embodiments of the present invention provides a method for preparing exosomes derived from three-dimensional cultured mesenchymal stem cells, which includes: seeding mesenchymal stem cells on the three-dimensional scaffold material, and performing adherent culture, and then Exosomes derived from three-dimensional cultured mesenchymal stem cells were isolated.

[0049] In some preferred embodiments, the three-dimensional scaffold material used in the present invention is a three-dimensional graphene foam scaffold material, of course, it can also be replaced with other biomaterials with three-dimensional spatial structure, porosity, and surface topology.

[0050] Furthermore, the mesenchymal stem cells in the present invention are derived from human umbilical cord mesenchymal stem cells, and of course can be replaced with mesenchymal stem cells from other species, other sources or other cell types from which exosomes can be obtained.

[0051] Further...

Embodiment 1

[0068] (1) Using the traditional CVD method to prepare two-dimensional graphene film and three-dimensional graphene foam scaffold: heat the clean copper foil to 950°C in an argon and hydrogen environment, and then expose it to hydrogen and methane at 950°C environment for 5 minutes, and then cooled to room temperature in an argon and hydrogen environment to prepare a two-dimensional graphene film; similarly, using metal nickel with a porosity of about 95% and a pore diameter of 30-300 μm as a template and a catalyst, at 950 The three-dimensional porous graphene scaffold with metal foam was prepared under the condition of methane as the carbon source; in a closed glass container, FeCl 3 The acidic solution is used as a corrosion solution to remove the metal catalyst, and it is washed with nitric acid solution and deionized water in sequence.

[0069] (2) Process the three-dimensional graphene foam support and the two-dimensional graphene film obtained in step (1) in an air plas...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses three-dimensionally cultured mesenchymal stem cells derived exosomes and a preparation method and application thereof. The preparation method of the three-dimensionally cultured mesenchymal stem cells derived exosomes comprises: inoculating mesenchymal stem cells to a three-dimensional support material, carrying out wall-attached culture, and isolating to obtain the three-dimensionally cultured mesenchymal stem cells derived exosomes. The invention also discloses application of the three-dimensionally cultured mesenchymal stem cells derived exosomes in the preparation of drugs to treat Alzheimer's disease. The three-dimensional support material is used as a carrier to culture mesenchymal stem cells, so that the yield of the mesenchymal stem cells derived exosomes can be increased, inclusion composition and functionality of the exosomes can be regulated, and particularly in the treatment of Alzheimer's disease, deposition of amyloid proteins can be inhibited effectively, and memory and cognitive capacities can be improved effectively.

Description

technical field [0001] The invention relates to a three-dimensional scaffold material and its application in the preparation of mesenchymal stem cell-derived exosomes, and the application of the obtained three-dimensional cultured mesenchymal stem cell-derived exosomes in the preparation of products for the treatment of Alzheimer's disease , belongs to the technical field of biomedical materials. Background technique [0002] Alzheimer's disease is one of the most common symptoms of senile dementia, manifested as a decline in cognitive level and memory function. In 2018, there were about 50 million patients worldwide, and this number will increase to 152 million in 2050. The main pathological features of Alzheimer's disease are the deposition of extracellular amyloid to form senile plaques and the hyperphosphorylation of intracellular Tau protein to form neuron tangles, which eventually lead to the loss of neuronal function and death. Currently, there is no clinically effe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/51A61P25/28A61P25/00
Inventor 杨令延程国胜翟源心朱展翅
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap