Test strip for detecting human ABO blood types, preparation method, detection method and application

An ABO blood type and test strip technology, applied in the field of biomedicine, can solve the problems of high professional technical level requirements of operators, low temperature preservation of supporting reagents, low sensitivity of chromatographic agglutination method, etc. Good effectiveness, easy and fast operation

Active Publication Date: 2019-06-21
迪佰(厦门)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art has the following defects, among them, the supporting reagents of the mixed agglutination method need to be stored at low temperature; the reagents of the micro-column gel method are cumbersome to operate and need to be centrifuged; the chromatography agglutination method has low sensitivity and is easy to miss detection, etc.
[0004] In addition, the existing technology has higher requirements on the professional technical level of the operators

Method used

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  • Test strip for detecting human ABO blood types, preparation method, detection method and application
  • Test strip for detecting human ABO blood types, preparation method, detection method and application
  • Test strip for detecting human ABO blood types, preparation method, detection method and application

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Embodiment 1

[0044] Preparation of test strips for detection of human ABO blood type and use for detection of ABO blood type of human blood samples.

[0045] (1) Pre-coat anti-A, anti-B, anti-RBC on nitrocellulose membrane (as quality control line).

[0046] refer to figure 1 As shown, a nitrocellulose membrane 300 with a size of 2.5cm*30cm was cut out, and a 3mg / ml solution of antibody A and antibody B was prepared with a coating buffer, and the coating buffer contained 30mmol phosphate buffer (PH=7.5), 1% sucrose, anti-A, anti-B and anti-RBC solutions with a concentration of 3mg / ml were spotted linearly as the detection line, the detection line A 520 of anti-A was 6mm away from the lower edge of the nitrocellulose membrane 300, the detection line of anti-B B 530 is 10 mm away from the lower edge of the nitrocellulose membrane 300 , and the other end of the nitrocellulose membrane 300 is linearly dotted with 3 mg / ml anti-RBC as the quality control line 540 .

[0047] (2) Preparation of ...

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PUM

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Abstract

A test strip for detecting human ABO blood types, a preparation method, a detection method and an application, which belong to the field of biological medicines. The test strip includes a polyester board, and absorbent paper bonded to the upper edge of the polyester board; and a sample pad and a nitrocellulose membrane pre-coated with A resistance, B resistance and RBC resistance are sequentiallylaid on the upper surface of the polyester board in a chromatographic direction, the upper edge of the nitrocellulose membrane is overlapped with the lower edge of the absorbent paper, and the samplepad is paved with a hemolytic agent and a cell membrane fluorescent dye. The invention provides a preparation method for the test strip for detecting the human ABO blood types, and the test strip in which a blood type detection supporting reagent does not low temperature preservation and which is simple in operation and high in sensitivity can be prepared through the preparation method. Moreover,the test strip for detecting the human ABO blood types is provided, and the test strip can complete joint detection of multiple antigen projects in a short time, is simple and quick in operation, is low in professional technical level requirement for operation personnel, is suitable for clinical detection, and is quick in obtainment of a test result.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a test strip for detecting human ABO blood type, a preparation method, a detection method and an application. Background technique [0002] A system of blood types based on the presence or absence of specific antigens (agglutinogens) A and B on the surface of red blood cells. The ABO blood group system is based on the distribution of agglutinogens A and B to divide blood into four types: A, B, AB, and O. Type A blood with only agglutinogen A on red blood cells, and anti-B lectin in its serum; type B blood with only agglutinogen B on red blood cells, and anti-A lectin in its serum; two types of A and B on red blood cells Type AB blood with both agglutinogens has no anti-A and anti-B agglutinins in its serum; type O blood without both A and B agglutinins on red blood cells has both anti-A and anti-B agglutinins in its serum . Red blood cells with agglutinogen A can be agglutinated by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/533G01N33/558G01N21/64
Inventor 钟乾兴肖江群王保丹
Owner 迪佰(厦门)生物科技有限公司
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