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Application of rosemary compound in drug for preventing and treating autoimmune hepatitis

An autoimmune, rosemary technology, used in the field of medicine to achieve the effect of reducing hepatitis/liver damage, widely distributed, and relieving hepatitis/liver damage

Inactive Publication Date: 2019-06-25
江西华普康明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the treatment of autoimmune hepatitis, corticosteroids alone or combined with immunosuppressants are still one of the main clinical treatment methods, but at the same time, acute liver injury caused by various drug adverse reactions is also commonly reported

Method used

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  • Application of rosemary compound in drug for preventing and treating autoimmune hepatitis
  • Application of rosemary compound in drug for preventing and treating autoimmune hepatitis
  • Application of rosemary compound in drug for preventing and treating autoimmune hepatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, the optimal concentration of rosmarinic acid in preventing and treating ConA-induced acute autoimmune hepatitis

[0035]Set ConA (15mg / kg), ConA+RA (30mg / kg), ConA+RA (60mg / kg), ConA+RA (120mg / kg) a total of 4 groups, 6 mice in each group, about 18 mice per mouse -22g. RA was administered by intraperitoneal injection, 2 hours after the injection, ConA was administered by tail vein injection, and the injection dose was 15 mg / kg. 8, 16, and 24 hours after the injection of ConA, blood was collected from the orbital venous plexus, 50 μL ± 10 μL; after standing at room temperature for 1.5-2 hours, 3500 rpm, 5 minutes, the upper serum was gently transferred to a new EP tube, and frozen at -20 °C. Serum levels of ALT and AST were detected using a transaminase detection kit, ALT: alanine aminotransferase, AST: aspartate aminotransferase. Test results such as figure 1 , figure 2 As shown, data are expressed as mean ± standard error (n=6, *P# P## P# p## p<0.01), w...

Embodiment 2

[0036] Example 2. Effectiveness of Optimum Rosmarinic Acid Concentration in Preventing and Treating ConA-Induced Autoimmune Hepatitis

[0037] Set Control, ConA (15mg / kg), ConA+RA (60mg / kg), RA (120mg / kg) a total of 4 groups, 6 mice in each group, each mouse about 18-22g. RA / PBS was administered by intraperitoneal injection, 2 hours after injection, ConA / PBS was administered by tail vein injection, and the injection dose was 15 mg / kg. 8h, 16h, and 24h after injection of ConA, blood was collected from the orbital venous plexus, 50μL±10μL; after standing at room temperature for 1.5-2h, 3500rpm, 5min, the upper serum was gently transferred to a new EP tube, and stored at -20°C. Serum levels of transaminases ALT and AST were detected using a transaminase detection kit. Test results such as Figure 3-8 As indicated, data are presented as mean ± standard error (n=6, *P<0.05, **P<0.01 for ConA). The results showed that ConA-induced autoimmune hepatitis increased serum transaminase...

Embodiment 3

[0038] Example 3, Effect of Rosmarinic Acid on the Survival Rate of ConA-Induced Autoimmune Hepatitis Mice

[0039] There are 3 groups including ConA (30mg / kg), ConA+RA (60mg / kg), and RA (120mg / kg), with 10 mice in each group, and each mouse weighs about 18-22g. RA / PBS was administered by intraperitoneal injection, 2 hours after injection, ConA / PBS was administered by tail vein injection, and the injection dose was 30 mg / kg. The mice were observed every 4 hours after ConA injection, and the remaining surviving mice were euthanized 28 hours later. The results were as follows: Figure 9 As indicated, survival rate (%) data are expressed as mean ± standard error (n=10, **p<0.01). The experimental results showed that the survival rate of the Control group was 100% all the time, and the survival rate of the ConA group dropped to 0% when ConA was injected with a lethal dose for 20 hours, while the survival rate of the ConA+RA group reached 60% at 16 hours after the injection of Con...

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Abstract

The invention provides an application of a rosemary compound in a drug for preventing and treating autoimmune hepatitis. The rosemary compound can significantly reduce ConA-induced hepatitis / liver injury, and inhibit the production of inflammatory cytokines, including tumor necrosis factor alpha (TNF-alpha), interleukin 6(IL-6), interferon-gamma(IFN-gamma) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), by reducing serum transaminase ALT / AST level, play an anti-inflammatory role and relieves hepatitis / liver injury. The invention also provides a drug for preventing and treating autoimmune hepatitis, which comprises the rosemary compound.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of rosemary compounds in medicines for preventing and treating autoimmune hepatitis. Background technique [0002] The liver is the largest digestive gland and the center of energy metabolism in the body. Hepatitis refers to the destruction of liver cells and liver function damage caused by various pathogenic factors. Common pathogenic factors are viruses, bacteria, parasites, chemical poisons, drugs, alcohol, autoimmune factors, etc. The cells are destroyed, and the function of the liver is damaged, causing a series of uncomfortable symptoms in the body, as well as abnormalities in liver function indicators. According to the etiology, it can be divided into viral, bacterial (such as amoeba), drug-induced, alcoholic, toxic, autoimmune, and non-alcoholic fatty. [0003] Usually, what we call hepatitis in our lives mostly refers to viral hepatitis ca...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P1/16A61P37/02
Inventor 王蒲汪亚兰邹晓华
Owner 江西华普康明生物科技有限公司
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