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Application of rppa and pwg in the preparation of respiratory pathogen detection products

A pathogen detection and respiratory technology, which is applied in the application field of RPPA and PWG in the preparation of respiratory pathogen detection products, and can solve the problems of easy missed detection and other problems

Active Publication Date: 2020-09-29
ZHEJIANG UNIV +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] Aiming at the technical problem that the prior art is prone to missed detection when the amount of protein contained in the sample is relatively small, the purpose of the present invention is to provide a method that is not easy to cause missed detection when the amount of protein contained in the sample is relatively small. Application of RPPA and PWG in the preparation of respiratory pathogen detection products

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  • Application of rppa and pwg in the preparation of respiratory pathogen detection products
  • Application of rppa and pwg in the preparation of respiratory pathogen detection products
  • Application of rppa and pwg in the preparation of respiratory pathogen detection products

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Embodiment Construction

[0071] As a high-sensitivity detection technology, biochip technology based on Planar Wave Guide (PWG) technology has incomparable advantages in the detection of protein molecules. The principle is that by engraving a grating structure in a planar solid-phase substrate (such as silicon dioxide, etc.), when light enters the surface of an optically sparse medium from an optically dense medium, when the incident angle meets certain conditions, the light will all be reflected back to the optically dense medium. Inside, that is, total reflection phenomenon. The phenomenon of total reflection will form a light field on the surface of the optically sparse medium, which is usually defined as an evanescent wave, and the height of the evanescent wave is 100 nanometers, while the binding height of biomolecules on the solid surface is generally tens of nanometers , so the molecules bound to the surface of the solid phase can be captured by fluorescence detection, while most of the impurit...

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Abstract

The invention discloses a respiratory pathogen detection method. The method includes the following steps that: 1) protein extraction is performed on a given respiratory pathogen sample; 2) a RPPA (reverse phase protein array) is formed with proteins extracted in the step 1) adopted as protein samples; and 3) microbial or virus detection is performed on the RPPA obtained in the step 2) through a PWG (planar wave guide). With the respiratory pathogen detection method of the invention adopted, the possibility of missed detection is small when the protein content of a sample is low.

Description

technical field [0001] The invention relates to an application of RPPA and PWG in preparing products for detecting respiratory pathogens. Background technique [0002] Influenza Virus (Influenza Virus), is a representative species of Orthomyxoviridae (Orthomyxoviridae), referred to as influenza virus, including human influenza virus and animal influenza virus, human influenza virus is divided into A (A), B (B), C ( C) three types, which are the pathogens of influenza (flu). Among them, the antigenicity of influenza A virus is easy to mutate, causing worldwide pandemics many times. For example, in the pandemic from 1918 to 1919, at least 20 million to 40 million people died of influenza all over the world; influenza B virus is also highly pathogenic to humans and can break out in local areas. [0003] Influenza A virus particles are pleomorphic, with a spherical diameter of 80-120nm and a capsule. The genome is segmented single-stranded negative-sense RNA. Influenza A vir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/531
Inventor 陈瑜王楠郑书发楼滨
Owner ZHEJIANG UNIV
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