Biochemical synergistic control medicament for grape downy mildew and application thereof

Abstract

The invention discloses a biochemical synergistic control medicament for grape downy mildew and an application thereof. The medicament comprises propamocarb and collybia radicata protein extract; thepreparation method of the collybia radicata protein extract comprises the following steps of: crushing collybia radicata fruiting bodies, leaching with water, and collecting water-soluble substances to obtain a the collybia radicata water-soluble extract; carrying out ammonium sulfate precipitation with saturation of 80% on the collybia radicata water-soluble extract, collecting the precipitate, and dialyzing the precipitate with deionized water to obtain the collybia radicata protein extract. The EC50 of the propamocarb and collybia protein extract to the to the grape downy mildew bacteria is0.21 mg / L and 0.07 mg / L respectively, the propamocarb t and the collybia radicata protein extract is mixed according to the mass ratio of 21: 490 and 21: 90 to generate synergistic effect, and the EC50 of the propamocarb to the grape downy mildew bacteria is 0.05 mg / L. The medicament has the advantage of reducing the usage amount of chemical agents in the aspect of treating and preventing the grape downy mildew.

Description

technical field [0001] The invention relates to the prevention and treatment of grape downy mildew in the field of biotechnology, in particular to a biochemical synergistic control agent for grape downy mildew and its application. Background technique [0002] Grape downy mildew is a worldwide grape disease. Grape downy mildew mainly damages leaves, and can also infect young tissues such as new shoots and young fruits. When the leaves are damaged, translucent, water-soaked, small spots with unclear edges appear on the leaf surface at first, and then gradually expand into light yellow to yellow-brown polygonal lesions, with different sizes and shapes, and sometimes several lesions are connected together , forming large dry spots of yellowish brown. When the air is humid, white moldy matter (spore peduncle and sporangia of pathogenic bacteria) is produced on the back of the lesion. The diseased spots are dry and brown, and the diseased leaves are easy to fall off early. Te...

AI Technical Summary

Problems solved by technology

The long-term high-volume use of chemical pesticides in grape production has caused a series of problems, such as the increase in the resistance of pathogenic bacteria / diseases and insects, fruit farmers have to increase the use of pesticides, and the cost of pesticides has increased; pesticide residues in fruits have exceeded the standard, and the risk of environmental pollution has increased There are many problems such as non-target biological damage and chronic toxicity, so grape production urgently needs high safety, low residue, and pollution-free pest control technology

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