Method for preparing r-4-chloro-3-hydroxybutanoate by utilizing coupling extraction of enzyme membrane reactor

Abstract

The invention relates to a method for preparing r-4-chloro-3-hydroxybutanoate by utilizing coupling extraction of an enzyme membrane reactor. The method adopts the enzyme membrane reactor, an organicsolvent charging barrel and a substrate charging barrel, wherein filter membranes are distributed inside the enzyme membrane reactor, one side of the upper end of the enzyme membrane reactor is connected with a feed liquid outlet, one side of the enzyme membrane reactor is provided with an organic solvent outlet, one side of the lower end of the enzyme membrane reactor is connected with a feed liquid inlet, and the other side of the enzyme membrane reactor is provided with an organic solution inlet. The method is simple in process, the emulsification phenomenon in the reaction and extraction processes is decreased, the yield is improved, the loss of an organic solvent is reduced, multiple times of extraction are not needed, the operation is simple and convenient, the large-scale industrialproduction is facilitated, a product can be continuously collected, the inhibition of the product to enzyme or the toxicity of the product to cells is relieved, the molar conversion rate is improved,and thus the industrial production is facilitated.

Description

technical field [0001] The invention relates to the technical field of enzyme engineering, in particular to a method for preparing ethyl r-4-chloro-3-hydroxybutyrate by coupled extraction with an enzyme-membrane reactor. Background technique [0002] Ethyl r-4-chloro-3-hydroxybutyrate is an important intermediate for the synthesis of L-carnitine and chiral compounds such as macrolactam A and (R)-γ-amino-β-hydroxybutyric acid (GABOB) body, the market demand is huge. [0003] The preparation of r-4-chloro-3-hydroxybutyric acid ethyl ester mainly adopts biological methods. At present, the commonly used method is to use microbial methods to prepare r-carbonyl reductase and coenzyme regeneration system, and then use these two enzymes to biocatalyze 4-chloro Ethyl acetoacetate synthesizes ethyl r-4-chloro-3-hydroxybutyrate. Since ethyl r-4-chloro-3-hydroxybutyrate is insoluble in water and has greater toxicity to cells / enzymes, it is generally Water / organic solvent two-phase sys...

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Problems solved by technology

At present, there is no document or patent report on how to eliminate the emulsification phenomenon. Only the patent document CN104651292A discloses a method of using a macroporous adsorption resin to remove the substrate COBE in situ and the product s-4-chloro-3-hydroxybutyric acid ethyl ester. method, but this method requires a large amount of eluent and requires resin regeneration, which will generate a large amount of waste water and solid waste, and the operation is cumbersome and the cost is relatively large.

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