Serum-free, animal-derived component-free, defined media and their applications

Abstract

The invention provides a serum-free, animal-source component-free medium with defined components and application thereof, and relates to the technical field of cell culture. The medium provided by the present invention is serum-free, animal-derived component-free, and defined components, including basal medium and cytokines, hormones, transferrin, albumin, fetuin, putrefaction, etc. added in the basal medium. Amines and serotonin. The invention realizes the cultivation of human cells by adding substances such as cytokines and hormones to the basal medium instead of serum. The components of the medium are clear, without the addition of serum and animal-derived components, which can alleviate the poor reproducibility of traditional serum-containing medium for cell culture, as well as the low viability and cell expansion of cells cultured in other serum-free medium Less efficient technical issues.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free, animal-derived component-free, well-defined culture medium and its application. Background technique [0002] Cell culture technology is an important and commonly used technology in cell biology research methods. By simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro, cells can survive, grow, reproduce and maintain their main structures and functions. Obtain a large number of cells to study signal transduction, anabolism, growth and proliferation of cells. Of all the ex vivo cell cultures, the most difficult is the human cell culture. [0003] The nutrients required for in vitro cell culture are basically the same as those in vivo, for example, sugars, amino acids, inorganic salts, growth-promoting factors, trace elements, etc. are required. The above-mentioned substances required by cells ...

AI Technical Summary

Problems solved by technology

Although the traditional culture medium added with serum and other animal-derived components can realize the in vitro culture of human cells, there are substances that damage cell growth and reproduction, such as complement, immunoglobulin and some growth inhibitory factors, and the components are not clear; traditional culture The reproducibility of the base for cell culture is also poor, which affects the safety and stability of clinical (human) application; and the serum activity of different animals and different batches is quite different, which affects the stability of the culture effect; at the same time, containing Cell viability and cell expansion efficiency are low in serum-based traditional media

In order to avoid the above-mentioned problems caused by the traditional culture medium, some scholars have developed a serum-free medium by adding a defined substance to the medium instead of serum. Although these serum-free medium solve the problem of traditional medium for human cell culture The problem of poor reproducibility has improved the safety and stability of clinical applications, but the cells obtained from it still have the problems of low cell viability and low cell expansion efficiency

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