A kind of ddt monoclonal antibody and its preparation method and application
A monoclonal antibody and antibody heavy chain technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of many operation steps, unintuitive detection results, long detection time, etc.
Active Publication Date: 2021-03-16
苏州诚检生物科技有限公司
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Problems solved by technology
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA in the rapid detection of DDT is greatly limited.
Method used
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Embodiment
[0035] Embodiment: a kind of preparation method of DDT monoclonal antibody comprises the following steps:
[0036] Step 1) Synthesis of DDT hapten:
[0037] Synthesis of 4-[4-(1-p-chlorophenyl-2,2,2-trichloro)phenyl]butanoic acid. Put 2.3g of chloral hydrate, 2mL of chlorobenzene and 1.6g of 4-phenylbutyric acid into a round bottom flask, and heat in a water bath until all the solids are dissolved. In an ice bath, slowly add 7 mL of 96% concentrated sulfuric acid, and shake for 1.5 h. After the reaction, slowly pour into 100mL water and stir. It was extracted with diethyl ether, dried and concentrated, and then purified by thin-layer chromatography with dichloromethane as the mobile phase to obtain 1.1 g of the product (yield 27%).
[0038] Step 2) Coupling of DDT hapten with carrier protein, and preparing coated antigen:
[0039] The immune antigen was prepared by the Active Ester method (AE method for short). Weigh 0.1mmol of the hapten 4-[4-(1-p-chlorophenyl-2,2,2-tric...
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Abstract
The invention relates to a DDT monoclonal antibody and a preparation method and application thereof. Compared with the prior art, the DDT monoclonal antibody and preparation method and application thereof have the advantages that (1) the specificity and sensitivity are high; 2) the method is simple and rapid, no other auxiliary instrument is needed when prepared colloidal gold test paper is used,onsite operation is realized, and a detection result can be determined in 3-5 minutes by placing a detection sample into a sample pad; 3) a result is displayed vividly, visually and accurately; and 4)the cost is reduced, the application range is wide, convenience is provided for popularization, the result can be determined within minutes according to strips presented after reaction needless of professionals or auxiliary instruments, operation is simple and rapid, the result is visual and accurate, the cost is low, and detection can be carried out in the outside.
Description
technical field [0001] The invention relates to the field of agricultural product detection, in particular to a DDT monoclonal antibody and its preparation method and application. Background technique [0002] DDT (Dichlorodiphenyltrichloroethane), also known as DDT, belongs to organochlorine insecticides. By poisoning the nervous system in insects, it first induces excitement in insects, and then blocks nerve conduction, leading to convulsions, paralysis, and death of insects. In the first half of the 20th century, the prevention of agricultural diseases and insect pests and the reduction of diseases transmitted by mosquitoes and flies such as malaria and typhoid played a significant role. However, due to its serious environmental pollution, many countries and regions have banned its use. The World Health Organization announced in 2002 that DDT was re-used to control the reproduction of mosquitoes and prevent malaria, dengue fever, yellow fever, etc. [0003] At present, ...
Claims
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IPC IPC(8): G01N33/559G01N33/553G01N33/577
Inventor 盛相国
Owner 苏州诚检生物科技有限公司