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A kind of polypeptide derivative that promotes cell proliferation and its preparation method and application

A technology of derivatives and polypeptides, which is applied in the field of polypeptide derivatives, polypeptide derivatives that promote cell proliferation and its preparation, can solve the problems of high production cost, low retention rate, and limited scope of application of recombinant proteins, so as to promote endothelial cell proliferation , easy to synthesize, and promote the effect of blood vessel repair and regeneration

Active Publication Date: 2022-08-02
克尔维特(福州)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] First of all: the production cost of existing recombinant proteins is high, the production process is difficult to strictly control, the quality between batches is difficult to guarantee, and industrialization is difficult
[0008] Secondly, the transportation and storage conditions of recombinant proteins are harsh, which further increases their production costs and limits their scope of application
[0009] Again, for the protein itself, it is ineffective to directly inject IGF-I protein into the damaged organ, mainly due to its low retention rate in the tissue (small size: about 7.5KD)
In order to solve these problems, researchers have developed many methods to encapsulate IGF-I and deliver it to designated locations through biocompatible carriers, but none of these studies have achieved clinical application

Method used

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  • A kind of polypeptide derivative that promotes cell proliferation and its preparation method and application
  • A kind of polypeptide derivative that promotes cell proliferation and its preparation method and application
  • A kind of polypeptide derivative that promotes cell proliferation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Solid Phase Synthesis of Nap-FFGGYGSSSRRAPQT

[0038] Specific steps are as follows:

[0039] 1) Weigh 0.5 mmol of 2-cl-Trt resin into a solid-phase synthesizer, add 10 mL of anhydrous dichloromethane (represented by DCM below), place it on a shaker and shake for 5 min to fully swell the 2-Cl-Trt resin ;

[0040] 2) Use ear washing balls to remove DCM from the solid-phase synthesizer equipped with 2-Cl-Trt resin;

[0041] 3) Dissolve 0.75 mmol of Fmoc-Thr in 10 mL of anhydrous DCM, add 0.75 mmol of DIPEA, then transfer to the above solid-phase synthesizer, add 0.75 mmol of DIPEA, and react at room temperature for 1 h;

[0042]4) Blocking: remove the reaction solution in the solid-phase synthesizer with ear washing balls, then wash with 10 mL of anhydrous DCM for 1 min each, wash 5 times in total, add the prepared volume ratio of anhydrous DCM:DIPEA:methanol= 17:1:2 solution 20mL, react at room temperature for 10min;

[0043] 5) Remove the reaction solution in the so...

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Abstract

The present invention relates to a polypeptide derivative that promotes cell proliferation, a preparation method and application thereof. The polypeptide derivative has the following structure: Nap-FFGGYGSSSRRAPQT. The present invention also provides a preparation method of the above-mentioned polypeptide derivative. The polypeptide derivative adopts FMOC-solid phase synthesis method. The present invention also provides the use of the above-mentioned polypeptide derivatives in medicines for promoting cell proliferation. The invention is simple to synthesize, and can synthesize active self-assembled polypeptides by solid-phase synthesis, and the raw materials used are amino acids necessary for the human body every day; secondly, because of its self-assembly, it can be slowly released in the body, which significantly solves the problem of IGF- After injection, protein I is easily degraded by various proteases in the body, which leads to the problem of low retention rate in the body; most importantly, it can bind to the IGF-I protein receptor, which in turn can stimulate downstream signaling pathways, promote endothelial cell proliferation, and promote Vascular repair and regeneration.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polypeptide derivative, in particular to a polypeptide derivative that promotes cell proliferation, and a preparation method and application thereof. Background technique [0002] Insulin-like growth factor I (IGF-I) is a growth factor protein similar to insulin in humans. It plays a vital role in growth and development, metabolism and cell survival, and has a wide range of applications in clinical and life science research. [0003] In clinical practice, there are currently two drugs based on IGF-I protein approved by the US FDA for the treatment of adolescent growth failure (Growth Failiure) and Laron syndrome (Laron syndrome), namely Mecasermin (Mecasermin) ), which is recombinant human IGF-I protein (rhIGF-I); and Mecasermin rinfabate, which is a complex of recombinant human IGF-I protein and recombinant human IGFBP-3 protein (rhIGFBP-3). [0004] In addition to being used as a c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/475C07K1/16C07K1/06C07K1/04A61K38/18A61P9/00B82Y5/00B82Y30/00B82Y40/00
CPCC07K14/475B82Y5/00B82Y30/00B82Y40/00A61K38/00Y02P20/55
Inventor 高洁杨志谋孔德领王恺商宇娜智登科
Owner 克尔维特(福州)医疗科技有限公司
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