Method of quantitative purification and preparation of deep sea foraminifer micro body samples

A production method and technology of foraminifera, applied in the preparation of test samples, measuring devices, sampling, etc., can solve the problems of low extraction rate of foraminifera in deep sea sediments, many impurities, and low water permeability, and achieve sample structure High integrity, water saving, low water seepage

Pending Publication Date: 2019-07-02
INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
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Problems solved by technology

[0002] Due to the conditions of low temperature, high pressure, and close to the depth of carbonate compensation, deep-sea sediments are mostly fine in texture, high in viscosity, low in water permeability, less in sand grains, hard when dry, and sticky when exposed to water. Therefore, the traditional extraction experiment lacks quantitative purification and preparation methods suitable for deep-sea samples, and the stainless steel screen used seriously damages the shell wall of

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  • Method of quantitative purification and preparation of deep sea foraminifer micro body samples

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Experimental program
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Embodiment 1

[0019] 1. Fixed staining

[0020] After the deep-sea sediment samples are collected, the samples are frozen or fixed with alcohol (70% concentration and above) on site, and then stained with tiger bengal solution. The frozen samples were first fixed with alcohol (70% concentration and above) during processing, and then stained with tiger bengal solution.

[0021] 2. Number and weigh the crystallization dish or beaker

[0022] Take the crystallization dish, wash it with water, and dry it in a drying oven at 60°C. Number the crystallization dish and weigh it (accurate to 0.01g), record the weight of the crystallization dish and the corresponding sample number in the sample form, and fill in the sampling record form.

[0023] 3. Sample drying and weighing

[0024] Take the original sediment sample and put it into a weighed crystal dish, record the number, weigh the wet weight of the sediment, dry the sediment sample completely in a 60°C drying oven, and weigh it to calculate t...

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Abstract

The invention relates to comparative analysis of bio-community structures and identification research on different types, in particular to a method of quantitative purification and preparation of deepsea foraminifer micro body samples. Collected deep sea sediments are fixedly dyed, sediment samples are then taken and dried, immersion and dispersion are then carried out, and the samples are transferred to a sieve with a pore size of 300 meshes to make a sandbag; the sandbag is washed and dried again, the samples in the sandbag are subjected to flotation concentration, sieving, and microscopicpreparation, and purified deep sea foraminifer micro body samples can be acquired. The method of the invention can effectively extract and purify foraminifer samples in the sediments, the samples arepreserved intact without deformation, the experiment operation process is simple, and compared with the existing experiment method, the method of the invention is higher in extraction rate and purification rate, better in sample integrity and shorter in program time.

Description

technical field [0001] The invention relates to comparative analysis of biological community structure and identification research of different species, in particular to a method for quantitative purification and production of deep-sea foraminifera micro-specimens. Background technique [0002] Due to the conditions of low temperature, high pressure, and close to the depth of carbonate compensation, deep-sea sediments are mostly fine in texture, high in viscosity, low in water permeability, less in sand grains, hard when dry, and sticky when exposed to water. Therefore, traditional extraction experiments lack quantitative purification and preparation methods suitable for deep-sea samples, and the stainless steel screens used seriously damage the shell walls of deep-sea foraminifera, resulting in technical obstacles such as incomparable data in later analysis. In addition, the common method has a low extraction rate of foraminifera from deep-sea sediments, many impurities, an...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/38G01N1/36G01N1/40G01N1/44G01N1/34
CPCG01N1/2806G01N1/30G01N1/34G01N1/36G01N1/38G01N1/40G01N1/44G01N2001/368
Inventor 类彦立李铁刚
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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