Oligotrophic denitrifying bacterium and process thereof for denitrifying low COD concentration sewage of sewage treatment plant

A denitrifying bacteria and oligotrophic technology, applied in the field of applied microbiology, can solve the problem of high processing cost, achieve the effect of short residence time, short operation process and saving operation cost

Active Publication Date: 2019-07-05
有研资源环境技术研究院(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, conventional deep denitrification treatment often needs to add carbon source, resulting in additional investment and high treatment cost

Method used

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  • Oligotrophic denitrifying bacterium and process thereof for denitrifying low COD concentration sewage of sewage treatment plant
  • Oligotrophic denitrifying bacterium and process thereof for denitrifying low COD concentration sewage of sewage treatment plant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Enrichment, isolation and purification of oligotrophic denitrifying bacteria

[0031] (1) culture medium

[0032] Solid medium:

[0033] Ammonium chloride 0.1g / L, sodium sulfate 0.1g / L, zinc sulfate 0.01g / L, magnesium sulfate heptahydrate 0.01g / L, potassium dihydrogen phosphate 0.01g / L, calcium chloride 0.01g / L, peptone 0.001 g / L, yeast extract 0.001g / L, serine 0.001g / L, leucine 0.001g / L, gellan gum 20g, pH7.4~7.6.

[0034] The above medium was sterilized in an autoclave at 121° C. for 20 minutes, then poured onto a plate, and cooled for later use. The solid medium was used for the isolation of oligotrophic denitrifying bacteria.

[0035] Liquid medium: ammonium chloride 0.1g / L, sodium sulfate 0.1g / L, zinc sulfate 0.01g / L, magnesium sulfate heptahydrate 0.01g / L, potassium dihydrogen phosphate 0.01g / L, calcium chloride 0.01g / L L, peptone 0.001g / L, yeast extract 0.001g / L, serine 0.001g / L, leucine 0.001g / L, pH7.0~7.5.

[0036] The above culture medium was sterilized i...

Embodiment 2

[0045] Identification of strains

[0046] Observe the colony morphology on the solid medium plate, and observe the morphology of the strain with an optical microscope, and then use PCR to amplify the 16S rDNA sequence of the strain and perform sequencing analysis and analysis. The 16S sequence obtained by sequencing is sent to the NCBI public database (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome) for comparison, the comparison result is Denitrobacillus licheniformis.

Embodiment 3

[0048] Step 1: Take the first-class B standard discharge sewage from Gaobeidian Sewage Treatment Plant, and adjust the pH to 7.0.

[0049] Step 2: put suspended microbial filler in the bioreactor, and lay the microbial filler in the bioreactor with a thickness ≥ 10 cm; inoculate the oligotrophic denitrifying bacteria cultured with the above medium, with a bacterial concentration of 10 8 pcs / mL, the amount of Biometek EM1 added relative to the bulk volume of the packing is not less than 2×10 11 -4×10 11 CFU / M 3 .

[0050] Step 3: The bioreactor operates anaerobically, the operating temperature is 30°C, and the hydraulic retention is 6 hours; the bioreactor effluents to obtain purified water.

[0051] The influent and treated effluent were tested, and the test results are shown in Table 1.

[0052] Table 1 (unit mg / L)

[0053] COD total nitrogen Ammonia nitrogen Total Phosphorus SS raw water 60 22 15 1.2 20 out of water 47 10 4 0.4 8 ...

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Abstract

The invention provides an oligotrophic denitrifying bacterial strain. The oligotrophic denitrifying bacterial strain is classified and named Denitrobacillus licheniformis EM1, is preserved in the China Center for Type Culture Collection in Wuhan University, Wuhan, China, is preserved on November 10, 2017, and has a preservation number of CCTCC No M2017678. The bacterium can survive under a low carbon source concentration and can efficiently realize the denitrifying process, so that upgrading and reconstruction of an urban sewage treatment plant can be implemented with low cost.

Description

technical field [0001] The invention belongs to the field of applied microbiology, and relates to an oligotrophic denitrifying bacterium and a process for upgrading and transforming a sewage treatment plant. Background technique [0002] With the process of urbanization and the accelerated development of industry in our country, environmental protection issues, especially urban sewage treatment, have become a research hotspot in various countries. However, as a large amount of domestic and industrial sewage flows into rivers, lakes or groundwater, it causes serious pollution to water bodies and affects fishery water and domestic water. Urban sewage pollution has become one of the important factors restricting the development of the country, so the country's discharge standards for sewage treatment plants are becoming more and more stringent. In response to the national call for energy conservation and emission reduction, more and more urban sewage treatment plants have upgr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/28C02F3/34C12R1/01
CPCC02F3/28C02F3/34C12N1/20C12N1/205C12R2001/01
Inventor 刘兴宇张明江谷启源崔兴兰温建康
Owner 有研资源环境技术研究院(北京)有限公司
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