Genetic molecular marking method for disease-resistant breeding of pigs, and application thereof

A molecular marker and purpose technology, applied in the field of genetic molecular markers of pig disease resistance breeding, can solve problems such as efficiency reduction, genetic improvement, and impact on production traits, and achieve the effects of low cost, reduced morbidity, and accelerated breeding process.

Pending Publication Date: 2019-07-05
YANGZHOU UNIV
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Problems solved by technology

In breeding work, diseases can reduce the efficiency of selection and affect the genetic improvement of production traits

Method used

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  • Genetic molecular marking method for disease-resistant breeding of pigs, and application thereof
  • Genetic molecular marking method for disease-resistant breeding of pigs, and application thereof
  • Genetic molecular marking method for disease-resistant breeding of pigs, and application thereof

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Embodiment Construction

[0031] The technical solution of the present invention will be described in further detail below through specific examples.

[0032] A genetic molecular marker method for pig disease-resistant breeding, comprising the following steps:

[0033] Step 1: Screening and determination of pig TLR5 gene variation sites

[0034] 1.1) Test samples: 122 large white pigs bred in Changzhou Kangle Agriculture and Animal Husbandry Co., Ltd.;

[0035] 1.2) Genomic DNA extraction: Take about 1.0g of ear tissue sample from each Large White pig, put it into a 1.5mL centrifuge tube, and use the phenol-chloroform method to extract genomic DNA. The extracted genomic DNA is dissolved in ultrapure water and passed through NanoDrop -1000 trace nucleic acid analyzer to measure its concentration and purity, and dilute it with ultrapure water to 100mg / μL according to its concentration;

[0036]1.3) PCR amplification of the target fragment of the TLR5 gene: using the genomic mixed DNA as the amplificati...

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Abstract

The invention discloses a genetic molecular marking method for disease-resistant breeding of pigs, and application thereof. The genetic molecular marking method includes the following steps of firstly, screening and determining the variation points of the TLR5 genes of the pigs; secondly, amplifying sequences comprising the determined variation points; thirdly, performing SSCP (single stranded conformation polymorphism) analysis on the amplified products of the second step, and comprehensively detecting the genotypes of pig core groups; fourthly, analyzing the correlation between individual genotypes and important cytokines representing general disease resistance, and selecting samples having three of four PCR-SSCP bands of the amplified products as advantageous individuals in the disease-resistant breeding. The genetic molecular marking method for the disease-resistant breeding of the pigs has high accuracy and can be used for mass detection, thereby providing an effective molecular marker breeding measure for improving the immune response capability and general disease resistance of piglets; when a genetic molecular marker is applied to the disease-resistant breeding, the breeding progress can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of pig genetic breeding and molecular marker-assisted selection, and in particular relates to a genetic molecular marker method and application for pig disease-resistant breeding. Background technique [0002] With the popularization of intensive feeding and management methods, diseases have brought huge economic losses to the pig industry. This huge economic loss is mainly due to the death of pigs, the reduction of production efficiency, the increase of medical expenses and the loss of products. In breeding work, diseases will reduce the efficiency of selection and affect the genetic improvement of production traits. In recent years, with the enhancement of people's awareness of green protection, the recognition of the green and healthy breeding industry and products in line with the principles of "5R" (Reduce, Reuse, Recycle, Rescue, Revaluate) has been strengthened, and only rely on vaccines and drugs to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12Q1/6888
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/124C12Q2565/131
Inventor 曹越郜重丞包文斌王海飞吴正常
Owner YANGZHOU UNIV
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