Substrate material for highly reproducible surface-enhanced Raman scattering and method of use thereof

Abstract

The invention provides a high-reproducibility surface enhanced Raman scattering substrate material, comprising a reinforcing agent, a hydrophobic carrier and a liquid film preparation plate. The reinforcing agent is a water solution containing nano-metal particles and is used for being mixed with a to-be-detected substance solution, thereby achieving a surface enhancement purpose. The hydrophobiccarrier has a hydrophobic surface and is used for placing a mixed solution drop composed of the reinforcing agent and the to-be-detected substance solution. A round through hole is set on the liquid film preparation plate. A hydrophilic layer for absorbing the mixed solution drop placed on the surface of the hydrophobic carrier to form supporting liquid film is set on an inner wall of the round through hole. The substrate material provided by the invention is simple in operation and low in cost. An enhancement effect is achieved. Detection cost is greatly reduced. Detection efficiency is improved. Signal reproducibility and detection sensitivity are improved.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection, in particular to a high-reproducibility surface-enhanced Raman scattering base material and a use method thereof. Background technique [0002] Qualitative and (semi)quantitative analysis of trace chemical and biological analytes is a field of analytical science that is closely related to social life, has great demand, and is extremely active in research. Since the discovery of the SERS effect, the development and quantitative analysis of highly reproducible SERS substrates have become the focus and hotspot in the research field. So far, the methods for fabricating Raman-enhanced substrates mainly include gold-silver electrode roughening, nanoparticle-induced aggregation, and nanoparticle assembly. The enhancement effect of the substrate obtained by electrode roughening is low, and the signal reproducibility is poor; although the enhancement effect of the substrate based on nanopar...

AI Technical Summary

Problems solved by technology

The enhancement effect of the substrate obtained by electrode roughening is low, and the signal reproducibility is poor; although the enhancement effect of the substrate based on nanoparticle aggregates is good, the signal reproducibility is poor; the enhancement effect of the substrate obtained by the method of nanoparticle assembly is good, The signal reproducibility is high, but this high enhancement effect and signal reproducibility often require the use of photolithography or electron (ion) beam etching technology, which makes the entire substrate preparation process complicated and expensive

In addition, the distribution of "hot spots" (locations of maximum electromagnetic field strength) in existing substrates is often inhomogeneous and, in many cases, difficult to quantify using SERS methods

Images