Acetamiprid active compound toxicity test method for earthworm propagation
A toxicity test, acetamiprid technology is applied in the field of toxicity test of acetamiprid original drug to earthworm reproduction, which can solve the problems of convincing experimental results, reducing reference data, unreasonable data analysis and processing, and improving analysis. Accuracy, Improve Accuracy, Improve Cross-Comparison
Inactive Publication Date: 2019-07-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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[0002] Acetamiprid is mainly used as an alkali insecticide, which has a nerve blocking effect on the synaptic receptors of pests. It is used in crops such as rice and vegetables, and has shown excellent activity against various aphids, whiteflies, rice leafhoppers and thrips , when studying the effect of acetamiprid on earthworms, it is necessary to test the earthworms. In the experiment of its effect on earthworm reproduction, the existing test methods are not systematic enough, and the comparison between the test group and the blank control group is not sufficient, which reduces the Reference data cannot improve the mutual comparison of data, cannot balance and offset the influence of irrelevant variables, cannot make the experimental results more convincing, cannot improve the accuracy of the data, and cannot improve the data analysis and processing during data processing. The accuracy of data analysis cannot improve the accuracy of test results
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[0047] During the test, the temperature was 19.5°C-21.4°C, the light intensity was 523Lux-689Lux, the light: dark time ratio was 16h:8h, the maximum water holding capacity of the test soil was 55.1%, the soil moisture content was 32.2% at the beginning of the test, and the pH value was 6.29-6.68, the water content was 31.6%-32.9% at the end of the test, and the pH value was 5.53-6.11. The mortality rate of each test container at the end of the first 4 weeks of exposure and the total number of vermicomposters at the beginning of the test were detected. Body weight, the percentage of surviving adult worms accounting for the initial body weight at the end of the first 4 weeks of the test, and the number of juvenile worms in each container at the end of the test are shown in Table 1. At the end of the first 4 weeks of exposure, each The vermicomb mortality rate in the test container was ≤10%, and the concentration of acetamiprid was 0.042-0.195mg a.i. / kg 干土 The average percentage...
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The invention discloses an acetamiprid active compound toxicity test method for earthworm propagation. The method comprises the steps that A a test sample and a reference substance are selected, wherein a 97% acetamiprid active compound is selected as the test sample, and 95% carbendazim is selected as the reference substance; and a blank control group is set at the same time. The method is efficient and accurate. Four replicates are set during each treatment. Ten earthworms are in each replicate. Eight replicates are in the control group. A test group and the blank control group are carried out at the same time. Reference data are added. Data cross-reference is improved. The influence of irrelevant variables is balanced and offset. The experimental result is more convincing. The data accuracy is improved. When the data are processed, DPS statistical analysis software is adopted. The data are reasonably analyzed and processed. The accuracy of data analysis is improved. The accuracy ofthe test result is improved.
Description
Technical field: [0001] The invention belongs to the technical field of toxicity test, in particular to the toxicity test method of acetamiprid original drug on earthworm reproduction. Background technique: [0002] Acetamiprid is mainly used as an alkali insecticide, which has a nerve blocking effect on the synaptic receptors of pests. It is used in crops such as rice and vegetables, and has shown excellent activity against various aphids, whiteflies, rice leafhoppers and thrips , when studying the effect of acetamiprid on earthworms, it is necessary to test the earthworms. In the experiment of its effect on earthworm reproduction, the existing test methods are not systematic enough, and the comparison between the test group and the blank control group is not sufficient, which reduces the Reference data cannot improve the mutual comparison of data, cannot balance and offset the influence of irrelevant variables, cannot make the experimental results more convincing, cannot i...
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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 王彦华柳新菊杨桂玲王新全王菲迪张昌朋王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES