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Weissella paramesenteroides and application thereof

A Weissella enteric membrane technology is applied in the application field of this type of Weissella enterica, which can solve the problems of chemical bactericide residue and microbial drug resistance, so as to broaden the application range and enrich the strain resources library effect

Active Publication Date: 2019-07-09
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical fungicides such as carbendazim, thiophanate-methyl, and imazalil are mainly used in the prevention and control of postharvest diseases of citrus fruits, but there are problems such as the residue of chemical fungicides and the tendency of microorganisms to develop resistance to them.

Method used

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  • Weissella paramesenteroides and application thereof
  • Weissella paramesenteroides and application thereof
  • Weissella paramesenteroides and application thereof

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Experimental program
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Embodiment Construction

[0014] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0015] 1. Screening of lactic acid bacteria

[0016] Citrus fruits, leaves, soil and peanut leaves were collected from Jinyun Mountain Citrus Orchard in Beibei District, Chongqing as samples. Each sample was completely submerged in 100mL MRS broth medium respectively, enriched and cultured at 37°C for 24 hours, and spread 10-fold serially diluted on a medium containing 0.3% CaCO 3 On the MRS plate, cultured at 37°C for 48 hours, a single colony with obvious calcium lysis circle was selected for isolation and purification, and microscopic examination and Gram staining were carried out. The purified strains were stored in a -80°C refrigerator.

[0017] The results are shown in Table 1. A total of 68 suspected strains were screened out, including 16 ...

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Abstract

The invention discloses weissella paramesenteroides JT13 with the preservation number of CGMCC No.17539, and application of the weissella paramesenteroides in preparing drugs for inhibiting penicillium digitatum and especially drugs for preventing green mold caused by penicillium digitatum after citrus fruits are harvested. The weissella paramesenteroides and the application thereof have the advantages that a lactic acid bacteria strain resource library is enriched, the application range of lactic acid bacteria in the field of fruit and vegetable preservation is widened, and a new solution isprovided for preventing and controlling the green mold after the citrus fruits are harvested.

Description

technical field [0001] The invention relates to a strain of Weissella enteriferii, and also relates to the application of the Weissella enterica. Background technique [0002] Lactic acid bacteria are a class of Gram-positive bacilli or cocci that can ferment sugars and whose main product is lactic acid. They have a good inhibitory effect on some Gram-positive or negative bacteria that are closely related to them. They are currently widely used in food fermentation, Fresh-keeping and other fields. However, there are few reports about the inhibitory effect of lactic acid bacteria on fungi. [0003] Citrus is a plant of the Citrus subfamily of Rutaceae. Citrus fruit is the fruit with the largest output in the world. During storage, it is prone to rot, edema, floating skin and other phenomena due to microbial infection and storage environment, which seriously affects the commercial value of the fruit. . Green mold caused by the fungus Penicillium digitatum is the main infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/01
CPCA23B7/155C12N1/205C12R2001/01
Inventor 曾凯芳马佳宏易兰花邓丽莉姚世响阮长晴
Owner SOUTHWEST UNIV
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