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Application of glutathione (GSH) to preparation of drug for preventing and treating central nervous system infection caused by salmonella typhimurium

A Salmonella, central nervous system technology, applied in the field of medicine, can solve problems such as intestinal flora disorder

Active Publication Date: 2019-07-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to reports, gentamicin has a better effect, but it may cause intestinal flora disturbance, so it poses a challenge to the development of drugs for the treatment of central nervous system infection caused by Salmonella typhimurium

Method used

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  • Application of glutathione (GSH) to preparation of drug for preventing and treating central nervous system infection caused by salmonella typhimurium
  • Application of glutathione (GSH) to preparation of drug for preventing and treating central nervous system infection caused by salmonella typhimurium
  • Application of glutathione (GSH) to preparation of drug for preventing and treating central nervous system infection caused by salmonella typhimurium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Application of GSH in the treatment of CNS infection caused by Salmonella typhimurium

[0013] Step 1: Experimental grouping (8-week-old C57 female mice, n=6)

[0014] i) Modeling group: pre-administered equal volume of normal saline (0.2mL / 20g) for 14 days. After infection, an equal volume of normal saline (0.2mL / 20g) was administered intragastrically.

[0015] ii) GSH pre-administration group for three days: after 11 days pre-administration of equal volume of normal saline (0.2 mL / 20 g), pre-administration of GSH (500 mg / kg*day) for 3 days. After infection, an equal volume of normal saline (0.2mL / 20g) was administered intragastrically.

[0016] iii) Seven-day GSH pre-administration group: after 7-day pre-administration of equal volume of normal saline (0.2 mL / 20 g), 7-day pre-administration of GSH (500 mg / kg*day). After infection, an equal volume of normal saline (0.2mL / 20g) was administered intragastrically.

[0017] iv) Fourteen-day GSH pre-administration group:...

Embodiment 2

[0027] Pharmacological Study of GSH in Treating CNS Infection Caused by Salmonella Typhimurium

[0028] Step 1: Experimental grouping (8-week-old C57 female mice, n=6)

[0029] i) Control group: an equal volume of normal saline (0.2 μL) was injected into the ventricle of the brain, and an equal volume of normal saline (0.2 mL / 20 g) was intragastrically administered after suturing.

[0030] ii) Modeling group: Salmonella typhimurium (65,000 CFU) was injected unilaterally into the ventricle, and an equal volume of normal saline (0.2 mL / 20 g) was intragastrically administered after suturing.

[0031] iii) Treatment group: Salmonella typhimurium (65000 CFU) was injected unilaterally into the ventricle, and an equal volume of GSH (500 mg / kg) was intragastrically administered after suturing.

[0032] Step 2: Establish a CNS infection model

[0033] i) Cultivation of Salmonella typhimurium SL1344: When cultivating the bacterial liquid, pick a single colony with a sterilized white g...

Embodiment 3

[0054] Study on the Mechanism of GSH Alleviating Cerebral Apoptosis

[0055] Step 1: Experimental grouping (8-week-old C57 female mice, n=6)

[0056] i) Control group: an equal volume of normal saline (0.2 μL) was injected into the ventricle of the brain, and an equal volume of normal saline (0.2 mL / 20 g) was intragastrically administered after suturing.

[0057] ii) Modeling group: Salmonella typhimurium (65,000 CFU) was injected unilaterally into the ventricle, and an equal volume of normal saline (0.2 mL / 20 g) was intragastrically administered after suturing.

[0058] iii) Treatment group: Salmonella typhimurium (65000 CFU) was injected unilaterally into the ventricle, and an equal volume of GSH (500 mg / kg) was intragastrically administered after suturing.

[0059] Step 2: Establish a CNS infection model

[0060] i) Culture of Salmonella typhimurium SL1344: When cultivating the bacterial liquid, pick a single colony with a sterilized white gun tip, pour into LB broth liqu...

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Abstract

The invention discloses application of glutathione (GSH) to preparation of a drug for preventing and treating central nervous system infection caused by salmonella typhimurium. A mouse meningitis model with the single-side ventricle being injected with the salmonella typhimurium SL1344 is provided, it is proven that the GSH has good treating activity, an experiment proves that the GSH can significantly prolong the median survival time of mice, the meningitis EAE score is increased, brain edema and inflammatory infiltration are relieved, and in addition, the GSH can relieve brain cell apoptosisby lowering phosphorylation of JNK.

Description

technical field [0001] The invention relates to the application of glutathione in the preparation of medicines for preventing and treating central nervous system infection caused by Salmonella typhimurium, belonging to the field of medicine. Background technique [0002] Glutathione (GSH) is a tripeptide that is combined with glutamic acid, cysteine ​​and glycine and contains a sulfhydryl group. It has antioxidant and integrated detoxification effects. The sulfhydryl group on cysteine ​​is the active group of glutathione (so glutathione is often abbreviated as G-SH), which is easy to interact with certain drugs (such as paracetamol), toxins (such as free radicals, iodoacetic acid, mustard gas, lead, mercury, arsenic and other heavy metals) etc., and has an integrated detoxification effect. Therefore, glutathione (especially glutathione in liver cells) can participate in biotransformation, thereby converting harmful poisons in the body into harmless substances and excreting ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 梁艳王广基沈博宇
Owner CHINA PHARM UNIV
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