Method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters

A cystamine-containing polyamidoamine and polyamidoamine technology is applied in the directions of instruments, analytical materials, measuring devices, etc., to achieve the effects of strong fluorescence, improved fluorescence intensity and good product stability

Active Publication Date: 2019-07-12
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the synthesis of different generations of dendritic sugar-containing polymers and the modification of the surface of gold nanoclusters have not been reported.

Method used

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  • Method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters
  • Method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters
  • Method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the synthesis (D 1 )

[0043] Add 373.5mg (1mmol) cystamine dihydrochloride and methanol into a round bottom flask, ultrasonically dissolve, vacuumize and blow nitrogen three times each, then place the flask in an ice-water bath; measure 0.71mL (8mmol) methyl acrylate In a centrifuge tube, add 0.9 mL of methanol, mix well, add dropwise into a round bottom flask with a syringe, and stir at room temperature for 24 hours after the dropwise addition is complete. After the reaction was completed, methanol and excess methyl acrylate were removed by rotary evaporation, and vacuum-dried at room temperature for 1 day to obtain D 0.5 (0.610 g, 85% yield). weighing D 0.5 (0.610g, 1.225mmol) and methanol were placed in a 25mL round-bottomed flask, and the flask was placed in an ice-water bath after vacuuming three times with nitrogen; measure 1.32mL (19.59mmol) ethylenediamine and 1.76mL methanol in a centrifuge tube Mix well and add dropwise to the round bottom f...

Embodiment 2

[0044] Embodiment 2: the synthesis (D 2 )

[0045] 0.874g (1.4mmol) D 1 Add methanol and methanol into a round bottom flask, evacuate nitrogen three times each, then place the flask in an ice-water bath; measure 1.95mL (22.8mmol) methyl acrylate into a centrifuge tube, and add 2.43mL methanol, mix well Add it dropwise into a round bottom flask with a syringe, and stir at 40°C for 72h after the dropwise addition. After the reaction was completed, methanol and excess methyl acrylate were removed by rotary evaporation, and vacuum-dried at room temperature for 1 day to obtain D 1.5 (1.298 g, 70% yield). weighing D 1.5 (1.298g, 0.994mmol) and methanol were placed in a 25mL round-bottomed flask, vacuumed three times with nitrogen and placed the flask in an ice-water bath; measure 2.12mL (31.8mmol) ethylenediamine and 2.83mL methanol in a centrifuge tube Mix well and add dropwise to the round bottom flask. After the dropwise addition was completed, the reaction was stirred at 5...

Embodiment 3

[0046] Embodiment 3: the synthesis (D 3 )

[0047] 1.46g (0.94mmol) D 2 Add methanol and methanol into a round bottom flask, evacuate nitrogen three times each, then place the flask in an ice-water bath; measure 2.67mL (30.08mmol) methyl acrylate into a centrifuge tube, and add 3.33mL methanol, mix well Add it dropwise into a round bottom flask with a syringe, and stir at 50°C for 96h after the dropwise addition. After the reaction was completed, methanol and excess methyl acrylate were removed by rotary evaporation, and vacuum-dried at room temperature for 1 day to obtain D 2.5 (1.328 g, 63% yield). weighing D 2.5 (1.328g, 0.454mmol) and methanol were placed in a 25mL round-bottomed flask, and the flask was placed in an ice-water bath after vacuuming three times with nitrogen; measure 2.91mL (43.6mmol) ethylenediamine and 3.88mL methanol in a centrifuge tube Mix well and add dropwise to the round bottom flask. After the dropwise addition was completed, the reaction was ...

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Abstract

The invention relates to a method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters, and in particular discloses a synthesis method of the arborescent lactose-containing polyamidoamine modified gold nano-clusters. The prepared gold nano-clusters can be used as a biosensor. The method comprises the following steps: synthesizing arborescent cystamine-containingpolyamidoamine of different generations through a Michael addition reaction, carrying out end group functionalization on the arborescent cystamine-containing polyamidoamine by using lactonolactone soas to obtain lactose group functionalized arborescent lactose-containing polyamidoamine Dm-Lac (m=1, 2, 3), and finally modifying the surface of gold nano-clusters with the arborescent lactose-containing polyamidoamine under the mutual actions of Au-S, thereby obtaining lactose-containing polyamidoamine modified gold nano-clusters (Dm-Lac@AuNCs) of different generations. The synthesis method provided by the invention is simple, and a simple and effective method is provided for preparing an efficient biosensor with thermosensitivity and targeting.

Description

technical field [0001] The invention relates to a method for preparing sugar-containing polyamidoamine-modified nanoclusters, in particular to a method for synthesizing dendritic lactose-containing polyamidoamine-modified gold nanoclusters, and the prepared gold nanoclusters can be used as biosensors. Background technique [0002] Gold nanoclusters (AuNCs) are usually composed of several to hundreds of gold atoms, and the diameter of the metal core is generally less than 2nm, with special physical and chemical properties, such as long fluorescence lifetime, ultra-small size, large Storr Kex shift, low cytotoxicity, easy to be functionalized with other biomolecules, strong photoluminescence, good biocompatibility and stability, etc. Among them, the most concerned by researchers is its unique fluorescent properties. However, metal nanoclusters that exist alone are prone to aggregation, thereby losing their luminescent properties. Therefore, it is necessary to select appropriat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00C08G73/02G01N21/64
CPCC08G73/028C08G83/001G01N21/6428
Inventor 戴晓晖常丹丹成的锴强昌武
Owner JIANGSU UNIV
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