65results about How to "Strong fluorescent properties" patented technology

The invention relates to a glutathione-Au/Ag alloy nano material, a preparation method and an application thereof. The preparation method includes following steps: 1) preparing a silver nano particle solution; 2) adding glutathione and performing an oil-bath reaction at 65-70 DEG C for 40-50 h, and adding deionized water with uniform stirring; 3) adding a chloroauric acid solution with stirring, centrifugally processing the mixed liquid, performing a mixed reaction to a supernatant with excessive methanol solution, and centrifugally processing the mixture again to obtain a precipitation, and 4) re-dissolving the precipitation in a PBS buffer solution to obtain a solution of the glutathione-Au/Ag alloy nano material. The glutathione-Au/Ag alloy nano material can be used in quantitative detection of histidine and immune-analysis of human chorionic gonadotropin. The preparation method is simple and convenient, wherein the glutathione is employed as a reduction agent and a stabilizer, so that the method is free of toxic reagents and is environment-friendly. The glutathione-Au/Ag alloy nano material is stable and uniform in structure, is good in dispersion property, is strong in fluorescence character, and can be used in analysis and detection of various bio-samples and detection of other relative functional materials.

The invention provides a red fluorescence gold nanocluster as well as a preparation method and an application thereof and belongs to preparation and applications of fluorescence nano materials. According to the preparation method, cheap egg white is used for rapidly reducing and coating HAuCl4, and gold nanocluster AuNCs@EW giving red fluorescence is synthesized. The preparation method is simple, rapid and low in cost. The prepared nanocluster can be used as fluorescent dye, can be used for detecting mercury ions and further can be used for detecting GSH (glutathione).

The invention relates to a preparation method for an inorganic-organic core-shell rare-earth (RE) polymer material, wherein core-shell RE-organic complex is a ternary RE-organic complexes LnM1 (M2R') 3R synthesized by a single substituted-benzoic acid compounds (L) and 1, 10-phenanthroline as ligand, as shown in the right figure. The said method includes: (1) preparation of organic RE complexes, (2) preparation of organic RE complexes emulsion, and (3) preparation of core-shell organic RE complexes/PMMA organic RE polymer light-conversion agent. The RE polymer composite has excellent characteristics of high optical conversion efficiency, strong fluorescence, longer fluorescence lifetime and complexes of high transparency, and can be used as a new optical materials in agriculture, biology, electrical and electronics, etc. arious fields. The preparation process is simple.

The invention provides a method for preparing bismuth telluride quantum dots by ultrasonic technology, which comprises the steps of: 1) taking a certain amount of solid bismuth telluride powder and fully grinding it in an agate mortar. 2) Add an appropriate amount of nitrogen-methylpyrrolidone (NMP) solution to the ground powder and mix, and place the above-mentioned mixture in an ultrasonic instrument for ultrasonication for a cumulative time of 4 hours. 3) Centrifuge the suspension system after ultrasonication, and collect the supernatant liquid to obtain the bismuth telluride quantum dot solution. The present invention adopts a very simple and low-cost preparation process to successfully obtain bismuth telluride quantum dots. The obtained quantum dots have good dispersion and can fluoresce under the irradiation of ultraviolet lamps. They are expected to be used in thermoelectric power generation, thermoelectric refrigeration and sensors, etc. fields are applied.

A photocatalyst with fluorescent core-shell nano-structure has an expression formula: SiO2@AxMy:RB, where x=0-2, y=0-3, A is chosen from Ti, Fe, Zn, Pb and Ag, M is chosen from O and S, and RB is rhodamine used as both fluorescent marker and surfactant. Its preparing process features that the spherical SiO2 particles with controllable diameter is used as core, and the dye rhodamine is embedded in the core-shell nano-structure.

A metal complex liquid crystal polymer and preparation method thereof, belongs to the technical field of polymer chemistry. The structure general formula of the metal complex liquid crystal polymer is (1), wherein R1 is cholesteric liquid crystal monomer; R2 and R3 respectively are -H and -OCnH2n +1 or -CnH2n +1; A1 is the soft segment comprising carbon 1 to 14; A2, A3 and A4 are respectively linkage group with conjugated structure; M is one of Al, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu. The product is prepared by taking the liquid crystal polymer containing carboxylic acid group as ligand and the metal ion as central ion, and adding carboxylic acid molecule to synthesize bonded metal liquid crystal polymer. The complex liquid crystal polymer not only maintains good liquid crystal performance, but also has excellent fluorescence property.

The invention discloses a degumming method applicable to green fluorescent protein-containing bave. The degumming method comprises the following steps: drying the bave to be constant-weight, soaking the bave in a pretreating solution in a bath ratio of 1: 50, degumming for 30-120 minutes under the conditions that the concentration of alkaline protease is 0.5-3g/L and the temperature is 40-60 DEG C, washing by water, and drying to obtain the degummed bave, wherein the pretreating solution is a solution with pH of 10 and containing 1g/L sodium carbonate and 0.5g/L penetrant JFC. The degumming method is suitable for degumming the green fluorescent protein-containing bave; by the degumming method, the degumming rate is high and the fluorescent characteristic of the bave can be retained to the maximum extent; after the bave is degummed through the degumming method, the bave has relatively small mechanical performance loss, soft hand feel and good mercerization; through the degumming method, a requirement on degumming of transgenic green bave can be met.

The invention belongs to the technical field of sensor detection, and particularly relates to preparation and application of a mercury ion fluorescence/electrochemical sensor. Preparation comprises the steps: a CQDs solution is prepared firstly, then EDC and NHS are added for the first time of stirring, then an amino modified mercury ion aptamer is added for the second time of stirring to obtain aspecific carbon quantum dot solution, a copper sulfate solution is added for the third time of stirring, and a carbon quantum dot and copper nano-cluster composite material is obtained by drying. According to a method for detecting mercury ions through the carbon quantum dot and copper nano-cluster composite nanomaterial and by constructing the fluorescence/electrochemical sensor, the mercury ions in food can be rapidly and accurately detected, and the quality safety of the food and the like is guaranteed advantageously.

The invention provides a method for preparing a nitrogen-doped graphene quantum point by using a reflux process. The method comprises the following steps: 1) adding acetylene black, concentrated nitric acid and deionized water in a certain ration into a reflux device, setting the temperature of a heating sleeve to be 90 to 100 DEG C and allowing a reaction solution to maintain slightly boiling for 3 to 20 h; and 2) after completion of a reflux reaction, cooling the reaction solution to room temperature, adjusting the pH value of the reaction solution with a sodium hydroxide solution until the reaction solution is neutral, carrying out separation and purification with a dialysis bag in deionized water and collecting the nitrogen-doped graphene quantum point. The method provided by the invention can easily prepare the nitrogen-doped graphene quantum point, is low in economic cost and simple in preparation technology and can realize preparation and doping of the graphene quantum point at the same time.

The invention relates to a silver nano-cluster fluorescent nanorod, a preparation method thereof, and an application of the nanorod in white LED. The fluorescent nanorod is formed by self-assembling Ag9 and hydrochloric acid in an aqueous solution. The Ag9 fluorescent nanorod prepared in the invention has outstanding optical properties, still maintains excellent fluorescent performances after being freeze-dried into a powder, can be used to prepare orange red light-emitting LED with a good stability, and can be mixed with a commercial blue phosphor and a commercial green phosphor according toa mass ratio of 10:5:1 to prepare the white light-emitting LED. The luminescent silver cluster can successfully replace the light conversion material of traditional materials in order to provide possibility for producing environmentally-friendly LEDs. The preparation method of the silver nano-cluster fluorescent nanorod of the invention has the advantages of simplicity and low cost; and the prepared LED has an excellent luminous intensity, and meets environmental protection requirements.

The invention provides double mode light-emitting water-based anti-counterfeiting ink, and a preparation method and application thereof. The preparation method comprises the steps that an organic solution of up-conversion nanoparticles and carbon quantum dots is added to water-in-oil microemulsion, then an organic solvent of the organic solution in the water-in-oil microemulsion is evaporated by heating, and then water is added to make the water-in-oil microemulsion inversely transform to form water-in-oil nano emulsion. According to the double mode light-emitting water-based anti-counterfeiting ink, and the preparation method and application thereof, the O/W nano emulsion is used for encapsulating artfully an oil-soluble double mode light-emitting material in the inner part of an emulsiondroplet, complex operation brought by removing ligands can be avoided, and the drawback of decreasing fluorescence intensity for removing hydrophobic ligands can be overcome. Compared with oil-soluble anti-counterfeiting ink, the water-based anti-counterfeiting ink has the advantages of no corrosion to a nozzle, no pollution to the environment and no blockage to the nozzle.

The invention relates to a tetra-branched rare earth complex and a preparation method thereof. The complex is prepared through complexing reaction of three ligands, namely two M1s and one M2 by taking a trivalent rare earth ion as a central ion. The chemical formula of the complex is Ln(M1)2M2, wherein Ln is the rare earth ion; M is the ligands consisting of M1 and M2; M1 is a monocarboxylic compound synthesized by a tetratomic alcohol compound and an aliphatic or aromatic dicarboxylic compound; M2 is 1,10-phenanthroline; and a ligand solvent is dimethyl sulfoxide or N,N-dimethyl formamide. The rare earth complex of the invention has a network pore structure, bigger conjugate plane, better rigid structure and structural stability, and more coordinative rare earth ions. A rare earth polymer luminescent material synthesized by the tetra-branched rare earth complex has no phenomenon that ions are accumulated to form ion clusters so as to cause fluorescent quenching, and can effectively perform high-efficiency photon switching to enhance the luminescent efficiency of the complex.

The invention discloses a thieno[3,4-b]indole derivative and a synthesis method thereof. The preparation method comprises the following steps: by taking 4-alkylthio-4-((2-(phenyl ethynyl)aryl)amino)butyl-3-ene-2-one as a raw material, catalyzing a self-cyclization reaction of 4-alkylthio-4-((2-(phenyl ethynyl)aryl)amino)butyl-3-ene-2-one with an oxidizing agent by catalyzing with a cheap metal toconstruct the thieno[3,4-b] indole derivative in one step, wherein the obtained thieno[3,4-b] indole derivative is widely applied to the fields of photoelectric materials, medicines and the like. Themethod of the invention has characteristics of cheap metal catalysis, elemental sulfur oxidation, easily available raw materials, simple operation, high efficiency, wide substrate adaptability and diversified functional groups.

The invention relates to a polycyclic aromatic hydrocarbon silane coupling agent with a strong fluorescence characteristic as well as green and efficient preparation and application thereof. The polycyclic aromatic hydrocarbon functionalized silane coupling agent with a strong fluorescence characteristic is efficiently prepared by utilizing a Diels-Alder reaction between a vinyl silane coupling agent and 7,9-diphenyl-8H-cyclopenta[a]acenaphthylen-8-one containing unsaturated bonds, tetraphenyl cyclopentadiene ketone and 1,3,6,8-tetraethynylpyrene, a catalyst does not need to participate in the reaction process, and the reaction process can be realized by only depending on the temperature. The method has a great industrial application prospect. The silane coupling agent can be used for preparing fluorescent polysiloxane and fluorescent cross-linked porous organic silicon resin, has wide application prospects in the aspects of fluorescent rubber, fluorescent porous membranes, super-hydrophobic porous silicon resin and the like, and is high in research and development value. According to the method disclosed by the invention, green and clean production of the functionalized silane coupling agent is realized by utilizing a catalyst-free Diels-Alder reaction, and a low-energy-consumption mild reaction technical approach is developed in the aspects of eliminating catalyst residues, simplifying post-treatment, increasing functionalized silane types and the like.

The invention discloses a method for preparing beta, beta'-binary (4-pyridyl) divinylbenzene, comprising the steps: phenyl-diiodide, 4-vinylpyridine and triethylamine are taken as raw material, and N, N'-dimethyl formamide is taken as solvent, and Heck reaction is carried out under the action of palladium catalyst at the temperature of 90-110 DEG C, so that the crude products of the beta, beta'-binary (4-pyridyl) divinylbenzene can be obtained; after the reaction is completely finished, the products are purified, and pure beta, beta'-binary (4-pyridyl) divinylbenzene can be obtained. The preparation method is simple and easy to do, and has mild reaction condition and extremely high production rate. The beta, beta'-binary (4-pyridyl) divinylbenzene can be taken as ligand of synthetic metal composition, cluster compound and coordination polymer material.

The invention relates to a method for preparing arborescent lactose-containing polyamidoamine modified gold nano-clusters, and in particular discloses a synthesis method of the arborescent lactose-containing polyamidoamine modified gold nano-clusters. The prepared gold nano-clusters can be used as a biosensor. The method comprises the following steps: synthesizing arborescent cystamine-containingpolyamidoamine of different generations through a Michael addition reaction, carrying out end group functionalization on the arborescent cystamine-containing polyamidoamine by using lactonolactone soas to obtain lactose group functionalized arborescent lactose-containing polyamidoamine Dm-Lac (m=1, 2, 3), and finally modifying the surface of gold nano-clusters with the arborescent lactose-containing polyamidoamine under the mutual actions of Au-S, thereby obtaining lactose-containing polyamidoamine modified gold nano-clusters (Dm-Lac@AuNCs) of different generations. The synthesis method provided by the invention is simple, and a simple and effective method is provided for preparing an efficient biosensor with thermosensitivity and targeting.

The invention belongs to the technical field of compound materials, and particularly relates to a fluorescence detection method of a total number of living bacteria. The fluorescence detection methodof the total number of the living bacteria comprises the following steps: conducting a mixing reaction on a to-be-detected sample, with an unknown number of the living bacteria, and fluorescent dye toobtain to-be-detected reaction liquid subjected to fluorescence staining; and detecting relative fluorescence intensity of the to-be-detected reaction liquid to obtain the number of the living bacteria in the to-be-detected reaction liquid. By means of the fluorescence detection method of the total number of the living bacteria, the number of the living bacteria which actually exist in an environment can be reflected, and the fluorescence detection method of the total number of the living bacteria is high in detection speed, convenient, simple, easy to operate, high in sensibility, accurate and good in dynamic range, can detect multiple kinds of microorganisms, and can achieve field monitoring.

The invention belongs to the field of immunology, and more specifically discloses a hybridoma cell strain, and an anti-chlamydia abortus monoclonal antibody secreted by the hybridoma cell strain. According to a preparation method, purified prokaryotically expressed chlamydia abortus major outer membrane protein (MOMP) is used for immunization of mouse as an antigen, indirect ELISA is adopted for screening of positive clones, and the hybridoma cell CA1-MM 01 capable of secreting the monoclonal antibodies aiming at chlamydia abortus major outer membrane protein is obtained. The identification ofthe monoclonal antibody in the aspects of the stability of the hybridoma cell after continuous continuous passage culture and cryopreservation, and the subtype and titer of the monoclonal antibody iscarried out, it is shown by results that the capacity of the hybridoma cell in secretion of antibodies is excellent and stable, the monoclonal antibody titer is high. It is shown that the obtained monoclonal antibody possesses extremely high specificity and combination capacity with chlamydia abortus.

The invention discloses a preparation method and application of an imine bond connected fluorescent covalent organic framework, and belongs to the technical field of environmental protection. The preparation method comprises the steps: carrying out a Schiff base reaction on 1,3,6,8-tetra (4-formyl phenyl) pyrene and 4,4'-diamino-[1,1'-biphenyl]-3,3'-diol to synthesize the imine bond connected fluorescent covalent organic framework. According to the fluorescent covalent organic framework, imine bonds serve as connecting units, a large number of hydroxyl functional groups exist around the connecting units, and uranyl ions can be selectively combined. Meanwhile, based on the excellent fluorescence characteristic of the pyrenyl unit, a rapid and sensitive detection method for uranyl ions is established. In addition, the phenolic hydroxyl group in the fluorescent covalent organic framework connected with the imine bond can also reduce soluble U (VI) into insoluble U (IV), so that the adsorption capacity of uranyl ions is greatly improved. The method for preparing the imine bond connected fluorescent covalent organic framework is simple, stable in structure and environment-friendly, can be used for high-sensitivity detection and selective recovery of the uranyl ions, and has a good application prospect.