Preparation method and application of mercury ion fluorescence/electrochemical sensor

Abstract

The invention belongs to the technical field of sensor detection, and particularly relates to preparation and application of a mercury ion fluorescence / electrochemical sensor. Preparation comprises the steps: a CQDs solution is prepared firstly, then EDC and NHS are added for the first time of stirring, then an amino modified mercury ion aptamer is added for the second time of stirring to obtain aspecific carbon quantum dot solution, a copper sulfate solution is added for the third time of stirring, and a carbon quantum dot and copper nano-cluster composite material is obtained by drying. According to a method for detecting mercury ions through the carbon quantum dot and copper nano-cluster composite nanomaterial and by constructing the fluorescence / electrochemical sensor, the mercury ions in food can be rapidly and accurately detected, and the quality safety of the food and the like is guaranteed advantageously.

Description

technical field [0001] The invention belongs to the technical field of sensor detection, and in particular relates to the preparation and application of a mercury ion fluorescence / electrochemical sensor. Background technique [0002] With the development of urban industry and the acceleration of urbanization, the discharge of mercury-containing industrial wastewater has caused increasingly serious environmental pollution. Mercury and its compounds are enriched in organisms through the biological chain and transferred to the human body through the food chain. The mercury ingested in the human body cannot be excreted through its own metabolism, and the accumulated mercury will lead to heart, liver and thyroid diseases, nervous system disorders, chronic mercury poisoning, and even malignant tumors. Therefore, the analysis and detection of mercury and its compounds has always been a research hotspot. [0003] Currently, mercury ions (Hg 2+ ) detection methods include spectrop...

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Problems solved by technology

[0003] Currently, mercury ions (Hg 2+ ) detection methods include spectrophotometry, atomic emission spectrometry, atomic absorption spectrometry, hydride generation-atomic fluorescence spectrometry, etc. These methods often have problems such as expensive instruments, long analysis period, complicated sample pretreatment, and expensive detection costs. Simple and rapid detection of mercury ions cannot be achieved

Compared with the previous traditional analysis and detection technology, the sensor detection technology based on nanomaterials has superior performance, such as less sample consumption, controllable detection range, portable equipment and simple operation, low cost, strong specificity and high sensitivity, etc. However, the current sensor technology is only based on a signal such as fluorescence, electrochemical or colorimetric signals to detect mercury ions, which has the disadvantages of poor stability and low detection range.

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