Glutathione-Au/Ag alloy nano material, preparation method and application thereof

A glutathione, gold-silver alloy technology, applied in nanotechnology, luminescent materials, nanotechnology and other directions, can solve the problems of complex process, unenvironmental protection, difficult control of stability and uniformity, etc., to achieve stable and uniform structure, avoid The effect of simple and convenient use and preparation of toxic reagents

Active Publication Date: 2015-11-11
SHANTOU UNIV
View PDF2 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of glutathione-gold-silver alloy nanomaterials to solve the problems t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof
  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof
  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] The preparation method of glutathione-gold-silver alloy nano material is as figure 1 As shown, including the following steps:

[0049] Step 1: Add 2.5 mL of 100 mM silver nitrate solution and 2.5 mL of 100 mM trisodium citrate solution to 39 mL of deionized water and stir evenly.

[0050] Step 2: Add 6 mL, 50 mM of freshly prepared sodium borohydride solution to the mixed solution in Step 1, and after stirring for 14 hours, a silver nanoparticle solution is obtained.

[0051] Step 3: Add 307.3 mg of glutathione powder to the silver nanoparticle solution obtained in step 2, and then react the mixture in an oil bath at 70°C for 50 hours to obtain a light orange solution, which is stabilized by glutathione Silver nanoparticle solution.

[0052] Step 4. Add 4 mL of the glutathione-stabilized silver nanoparticle solution from step 3 to 10.8 mL of deionized water and stir evenly; then slowly add 1.2 mL of 10 mM chloroauric acid solution (HAuCl 4 ·4H 2 O), continue t...

Example Embodiment

[0055] Example 2

[0056] The preparation method of glutathione-gold-silver alloy nano material is as figure 1 As shown, including the following steps:

[0057] Step 1. Add 2.64 mL of 100 mM silver nitrate solution and 2.64 mL of 100 mM trisodium citrate solution to 41.25 mL of deionized water and stir evenly.

[0058] Step 2: Add 7.93 mL, 50 mM freshly prepared sodium borohydride solution to the mixed solution in step 1, and keep stirring for 12 hours. After stirring overnight, a silver nanoparticle solution was obtained.

[0059] In step three, 307.3 mg of glutathione powder was added to the silver nanoparticle solution obtained in step two, and then the mixture was reacted in an oil bath at 65°C for 40 hours to obtain a light orange solution, which was stabilized by glutathione Silver nanoparticle solution.

[0060] Step 4. Add 4 mL of the glutathione-stabilized silver nanoparticle solution from step 3 to 10.8 mL of deionized water and stir evenly; then slowly add 1.2 mL of 10 mM c...

Example Embodiment

[0063] Example 3

[0064] The glutathione-gold-silver alloy nanomaterials prepared according to the methods of Example 1 and Example 2 were subjected to TEM, fluorescence, infrared, ultraviolet, XRD and SEM related characterization.

[0065] Such as figure 2 The three-dimensional fluorescence spectrum of glutathione-gold-silver alloy nanomaterials shown. It can be seen from the figure that the gold-silver alloy fluorescent nanomaterial prepared by the present invention has relatively strong fluorescent characteristics.

[0066] Such as image 3 with Figure 4 The TEM image and particle size distribution diagram of the glutathione-gold-silver alloy nanomaterials shown, when glutathione is used as a stabilizer and surface protectant, the gold-silver alloy nanomaterials are uniformly and stably distributed, and the particles The diameter distribution is uniform and the size is about 28.5nm.

[0067] Such as Figure 5 Shown are the optimized relationship diagrams of the fluorescence int...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a glutathione-Au/Ag alloy nano material, a preparation method and an application thereof. The preparation method includes following steps: 1) preparing a silver nano particle solution; 2) adding glutathione and performing an oil-bath reaction at 65-70 DEG C for 40-50 h, and adding deionized water with uniform stirring; 3) adding a chloroauric acid solution with stirring, centrifugally processing the mixed liquid, performing a mixed reaction to a supernatant with excessive methanol solution, and centrifugally processing the mixture again to obtain a precipitation, and 4) re-dissolving the precipitation in a PBS buffer solution to obtain a solution of the glutathione-Au/Ag alloy nano material. The glutathione-Au/Ag alloy nano material can be used in quantitative detection of histidine and immune-analysis of human chorionic gonadotropin. The preparation method is simple and convenient, wherein the glutathione is employed as a reduction agent and a stabilizer, so that the method is free of toxic reagents and is environment-friendly. The glutathione-Au/Ag alloy nano material is stable and uniform in structure, is good in dispersion property, is strong in fluorescence character, and can be used in analysis and detection of various bio-samples and detection of other relative functional materials.

Description

technical field [0001] The invention relates to the technical field of inorganic nanomaterials, in particular to a glutathione-gold-silver alloy nanomaterial and its preparation method and application. Background technique [0002] In the research of nanomaterials in recent years, noble metal nanomaterials have gradually shown their superiority in the detection of DNA, proteins, metal ions, hydrogen peroxide, toxic anions and thiols, and have attracted more and more researchers. more interest. But at the same time, a series of preparation methods, such as the use of polyols and microwave assistance, will be applied in the preparation of gold or silver-based nanomaterials. However, these preparation methods not only require high-temperature reactions, but also cause a lot of inconvenience because of the need to use microwave generators. Compared with the form of single metal components, multi-component nanomaterials such as gold-silver nano-alloy materials have the potentia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09K11/58B82Y30/00B22F9/24G01N21/64G01N33/76G01N33/68
Inventor 高文华黄晓鹏陈耀文陈汉佳张歆谢晓娜梁一丹
Owner SHANTOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap