Method for preparing fluorescent carbon nanoparticles by spontaneous reaction

A technology of carbon nanoparticles and fluorescence, which is applied in the direction of luminescent materials, nanotechnology, fluorescence/phosphorescence, etc., can solve the problems of cumbersome operation, low yield, unstable fluorescence emission wavelength, etc., and achieve broad application prospects, low cost, and excellent quality The effect of optical properties

Inactive Publication Date: 2015-07-22
CHINA PHARM UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

The preparation of carbon quantum dots by the above method often requires high temperature heating, or requires conditions such as voltage and strong acid, and the operation is relatively cumbersome, the yield is low, and carbon quantum dots cannot be prepared in large quantities; change, the fluorescence emission wavelength cannot be stabilized

Method used

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  • Method for preparing fluorescent carbon nanoparticles by spontaneous reaction
  • Method for preparing fluorescent carbon nanoparticles by spontaneous reaction
  • Method for preparing fluorescent carbon nanoparticles by spontaneous reaction

Examples

Experimental program
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Effect test

example 1

[0026] The preparation of fluorescent carbon nanoparticles of the present invention is as follows:

[0027] (1) Preparation of cyclodextrin aqueous solution: Weigh 0.2g of β-CD into a 5mL EP tube, add 2mL of distilled water into the EP tube with a pipette gun, and disperse it by ultrasonication;

[0028] (2) Preparation of fluorescent carbon nanoparticles: Weigh 2.5g P 2 o 5 In a 50mL Erlenmeyer flask, shake it so that it spreads flat on the bottom of the flask; to increase the P 2 o 5 The area in contact with the β-CD solution is sufficient to make the reaction fully; quickly pour the β-CD aqueous solution in the EP tube obtained in step (1) into the Erlenmeyer flask, an exothermic reaction occurs, white mist is generated, and the bottom of the bottle becomes brown-black Viscous liquid, the mixture gradually cooled to room temperature after 10 min;

[0029] (3) Centrifugal separation of fluorescent carbon nanoparticles: add 10 mL of distilled water to the Erlenmeyer flask...

example 2

[0032] (1) Preparation of cyclodextrin aqueous solution: Weigh 0.4g α-CD into a 5mL EP tube, add 4mL distilled water into the EP tube with a pipette gun, and disperse it by ultrasonic;

[0033] (2) Preparation of fluorescent carbon nanoparticles: Weigh 4.0g P 2 o 5 In a 50mL Erlenmeyer flask, shake it so that it spreads flat on the bottom of the flask; to increase the P 2 o 5 Contact area with α-CD solution to make the reaction fully; quickly pour the β-CD aqueous solution in the EP tube obtained in step (1) into the Erlenmeyer flask, an exothermic reaction occurs, white mist is generated, and the bottom of the bottle becomes brown-black Viscous liquid, the mixture gradually cooled to room temperature after 10 min;

[0034] (3) Centrifugal separation of fluorescent carbon nanoparticles: add 20mL of distilled water to the Erlenmeyer flask in step (2), shake and mix, add the mixed solution to 12 2mL EP tubes with a pipette gun; put the EP tube after adding the liquid Put it ...

example 3

[0039] After co-incubating the fluorescent carbon nanoparticles obtained in Example 1 with polyethylene glycol and co-incubating with human breast cancer MCF-7 cells, the fluorescence of the cell membrane and cytoplasm of MCF-7 cells was observed by two-photon fluorescence microscopy. Most obviously, it is speculated that the carbon nanoparticles can be transported to the interior of the cell through endocytosis, and can be used as bioluminescent probes for in vitro cell imaging; in addition, carbon nanoparticles bonded to membrane translocation peptides such as HIV-1 trans On the transcriptional activator (TAT), carbon nanoparticles can be well imaged in the nucleus.

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Abstract

The invention discloses a simple preparation method of fluorescent carbon nanoparticles and an application in cell imaging. The invention belongs to the technical field of a preparation technology of a carbon nanomaterial. By a chemical oxidation method, cyclodextrin is used as a carbon source to prepare a cyclodextrin solution; and the cyclodextrin solution is added into an oxidizing agent to prepare the fluorescent carbon nanoparticles by a one-step method. The preparation method is a process of a spontaneous reaction. No external heating or complex operation is required. White fog is generated after reactants are mixed. After the reaction, a lot of carbon nanoparticles can be obtained through centrifugation. The preparation method is pollution-free, simple, reliable and low-cost. Raw materials are easily available. The preparation method has a wide application prospect in fields of detection of heavy metal ions in the environment and cell imaging, etc.

Description

technical field [0001] The invention belongs to the technical field of nano-carbon material preparation technology, and specifically relates to the preparation of novel fluorescent carbon nano-particles, which are used as biological fluorescent probes for in vivo or in vitro cell imaging. Background technique [0002] Cyclodextrin (Cyclodexdrin, CD), also known as cyclohexyl group, is a compound formed by 6-8 molecules of D-glucose using α-1, 4 chains for cyclic connection. It is produced by cyclodextrin glucosyltransferase ( CGT) acts on a group of cyclic oligosaccharides produced by starch. First discovered in 1891. Schrödinger completed the research to determine the structure of CD. Since CD has a molecular structure of "hydrophobic on the inside and hydrophilic on the outside", and because CD is a chiral compound, this special molecular structure endows CD with the ability to form inclusion complexes with various guest compounds. , thus forming host-guest molecular che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02C09K11/65B82Y40/00G01N21/64
Inventor 何华肖得力李思桥左朋礼
Owner CHINA PHARM UNIV
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