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Application of biological dye namely xylene cyanol ff to membrane feeding of thrips type insects

A technology of xylene aniline and biological dye, which is applied in the field of insect organism dyeing and can solve problems such as insect poisoning and influence on scientific research.

Active Publication Date: 2019-07-19
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xylene aniline is a substance with minimal toxicity in the acute toxicity test of fish, and can be used as a redox indicator (orange-yellow to yellow), nucleic acid electrophoresis indicator or biological staining agent, but the amount of use needs to be accurately determined Control, so as not to cause poisonous effects on insects and affect scientific research

Method used

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  • Application of biological dye namely xylene cyanol ff to membrane feeding of thrips type insects
  • Application of biological dye namely xylene cyanol ff to membrane feeding of thrips type insects
  • Application of biological dye namely xylene cyanol ff to membrane feeding of thrips type insects

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Dissolve xylene aniline biological dye powder in 0.01mol / L phosphate buffer solution, 100mg / ml biological dye solution;

[0025] (2) Mix 100mg / ml xylene aniline biostaining agent and 0.01mol / L phosphate buffer solution with a pH value of 7.0 at a volume ratio of 1:9 to obtain a biostaining solution with a concentration of 10mg / ml;

[0026] (3) Film-fed the thrips nymphs with the biological dyeing solution in step (1), and fed them for 24 hours under the conditions of 25°C, 75% humidity, and 14L:10D light to obtain dyed thrips nymphs;

[0027] (4) Taking photos and imaging the staining conditions of the marked thrips nymphs in the step (2) with a microscope.

Embodiment 2

[0029] (1) Mix 100mg / ml xylene aniline biostaining agent and 0.01mol / L phosphate buffer solution with a pH value of 6.5 at a volume ratio of 1:9 to obtain a biostaining solution with a concentration of 10mg / ml;

[0030] (2) Film-feed the thrips nymphs with the biological dyeing solution in step (1), and feed them for 24 hours at 28°C, humidity 75%, light 14L:10D, and obtain dyed thrips nymphs;

[0031] (3) Taking photos and imaging the dyeing conditions of the marked thrips nymphs in the step (2) with a microscope.

Embodiment 3

[0033] (1) Mix 100 mg / ml xylene aniline biological dye and 0.01 mol / L phosphate buffer solution with a pH value of 7.8 at a volume ratio of 1:9 to obtain a biological staining solution with a concentration of 10 mg / ml;

[0034] (2) Film-fed the thrips nymphs with the biological dyeing solution in step (1), and fed them for 24 hours at 28°C, 75% humidity, and 14L:10D light conditions to obtain dyed thrips nymphs;

[0035] (3) Taking photos and imaging the dyeing conditions of the marked thrips nymphs in the step (2) with a microscope.

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Abstract

The invention provides an application of biological dye namely xylene cyanol ff to membrane feeding of thrips type insects. The application comprises specific steps of dissolving xylene cyanol ff biological dye powder in a 0.01mol / L phosphoric acid buffered solution of which the pH value is 7.0-7.4 to obtain a 1-10mg / ml biological dye solution, and performing membrane feedong on thrips nymphs in single-tube feeding so as to obtain dyed thrips nymphs. According to the application disclosed by the invention, the xylene cyanol ff is used as an indicator during membrane feeding of the thrips typeinsects, the xylene cyanol ff can be non-specifically combined with organism protein, under safe concentration, a direct evidence can be provided for accurately judging foraging of the thrips type insects, and a thrips type pest RAN interference technique system is quickly and conveniently established.

Description

technical field [0001] The invention belongs to the field of insect organism dyeing, and in particular relates to the application of a biological dye xylene aniline in thrips insect membrane feeding. Background technique [0002] Thrips palmi Karny belongs to the genus Thrips in the family Thripidae in the order Thysanoptera. Thrips palmi is a dangerous worldwide customs quarantine pest, which can harm more than 600 kinds of plants, and it is even more harmful to many important agricultural economic crops. In the 1970s, Thrips palmi was discovered for the first time in Taiwan, my country. Since then, it has occurred successively in Guangdong, Hunan, Sichuan, Yunnan, Tibet, Shandong, Beijing and other regions. [0003] At present, there are few studies on thrips feeding, limited to basic research such as gene cloning, protein induction expression and gene temporal and spatial distribution, but no research on membrane feeding has been reported. The present invention uses xyle...

Claims

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Application Information

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IPC IPC(8): A23K50/90A23K20/179
CPCA23K50/90A23K20/179Y02A50/30
Inventor 陈勇魏辉田厚军陈艺欣林硕
Owner INST OF PLANT PROTECTION FAAS
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