A kind of anti-ttc36 monoclonal antibody and its application
A monoclonal antibody, TTC36 technology, applied in the field of hybridoma cells, can solve the problems of the sale of monoclonal antibodies of TTC36 protein, not many, and no literature reports yet.
Inactive Publication Date: 2020-10-27
深圳市龙华区人民医院 +1
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[0004] At present, there are not many research reports on TTC36 protein. There are some polyclonal antibodies against TTC36 on the market, but there is no literature report on the use of TTC36 monoclonal antibody research, and there is no monoclonal antibody against human TTC36 protein on the market.
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[0027] The principles and features of the present invention will be described below with reference to the accompanying drawings. The examples are only used to explain the present invention, but not to limit the scope of the present invention.
[0028] 1. Prokaryotic expression of TTC36 protein
[0029] 1.1 Synthesis and cloning of gene fragments
[0030] The whole coding gene of TTC36 was codon-optimized, and the coding gene of GST tag was connected to the C-terminus to facilitate purification, and the obtained sequence was shown in SEQ ID NO: 1. EcoR I and Xho I sites were added at both ends of the sequence to facilitate cloning.
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The invention relates to a hybridoma cell deposited on October 17, 2018 in the China Center for Type Culture Collection (CCTCC) with the accession number CCTCC NO: C2018196, and further relates to a monoclonal antibody generated by the hybridoma cell and application thereof and a method for detecting a TTC36 protein in a sample for non-diagnostic purposes. The antibody can efficiently and specifically binds to the TTC36 protein in the sample, and has higher specificity and affinity than a polyclonal antibody. Better antibody tools are provided for TTC36 protein function studies, and research,diagnosis and treatment of related diseases.
Description
technical field [0001] The present invention relates to a hybridoma cell, an antibody produced by the hybridoma cell and application thereof, and a method for detecting TTC36 protein in a sample for non-diagnostic purposes. Background technique [0002] TTC36 (tetratricopeptide repeat domain 36) protein contains three TRP motifs (tetratricopeptide) and no other domains, belonging to TPR-like structural protein. Through phylogenetic tree analysis, we found that the amino acid sequence of TTC36 is highly conserved in mammals, which shows that it performs important functions in organisms. [0003] Jiang, L. et al. found that the expression of TTC36 protein was down-regulated in human hepatocellular carcinoma tissue specimens. If TTC36 gene was overexpressed in hepatoma cells, it could promote the apoptosis of hepatoma cells. James A Nathan's research shows that TTC36 may be involved in intracellular protein ubiquitination. [0004] At present, there are not many research repo...
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Login to View More IPC IPC(8): C07K16/18C12N5/20G01N33/574G01N33/577
CPCC07K16/18G01N33/57438G01N33/577
Inventor 刘运洪吴文权刘爱胜郭龙华韦爱平魏建伟陈雪艳
Owner 深圳市龙华区人民医院
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